Oliver Thon
@thonoliver.bsky.social
PhD Student @ Hänelt lab, Goethe University Frankfurt
Reposted by Oliver Thon
1/14
Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵
Free article: go.nature.com/4oRUC1q
Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵
Free article: go.nature.com/4oRUC1q
Evolutionary analysis reveals the origin of sodium coupling in glutamate transporters - Nature Structural & Molecular Biology
Reddy et al. used ancestral protein reconstruction, cryo-electron microscopy and functional assays to elucidate how a secondary active transporter evolved to harness the energy of sodium gradients to ...
go.nature.com
September 12, 2025 at 12:08 AM
1/14
Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵
Free article: go.nature.com/4oRUC1q
Happy (but mostly relieved) to share my dream project with @boudkerlab.bsky.social, now published in @natsmb.nature.com! We used evolution, protein engineering & cryoEM to uncover how ion coupling in glutamate transporters works, and how it evolved.🧵
Free article: go.nature.com/4oRUC1q
Reposted by Oliver Thon
Last week, we had the pleasure of welcoming Prof. Syma Khalid. PhD representatives of the IRTG board organised a week of talks & events. Syma shared her expertise in MD simulation, science communication & insights into her own research career. Thank you for such inspiring days! @sykhalid.bsky.social
August 29, 2025 at 8:48 AM
Last week, we had the pleasure of welcoming Prof. Syma Khalid. PhD representatives of the IRTG board organised a week of talks & events. Syma shared her expertise in MD simulation, science communication & insights into her own research career. Thank you for such inspiring days! @sykhalid.bsky.social
Reposted by Oliver Thon
🎉We’re celebrating the #SCALE-it-Best-PhD-Awards-2025, honoring exceptional researchers! 🎓 This year, three remarkable dissertations were recognized, selected from a highly competitive and multidisciplinary pool of candidates. 🧵
June 5, 2025 at 9:41 AM
🎉We’re celebrating the #SCALE-it-Best-PhD-Awards-2025, honoring exceptional researchers! 🎓 This year, three remarkable dissertations were recognized, selected from a highly competitive and multidisciplinary pool of candidates. 🧵
Reposted by Oliver Thon
🎉 We’re absolutely thrilled: SCALE has been selected as a DFG Excellence Cluster!
A huge thank you to our incredible team—this would not have been possible without your dedication and talent. 🙌 @dfg.de
#ClustersOfExcellence #SCALEcluster 🧵
A huge thank you to our incredible team—this would not have been possible without your dedication and talent. 🙌 @dfg.de
#ClustersOfExcellence #SCALEcluster 🧵
May 23, 2025 at 7:27 AM
🎉 We’re absolutely thrilled: SCALE has been selected as a DFG Excellence Cluster!
A huge thank you to our incredible team—this would not have been possible without your dedication and talent. 🙌 @dfg.de
#ClustersOfExcellence #SCALEcluster 🧵
A huge thank you to our incredible team—this would not have been possible without your dedication and talent. 🙌 @dfg.de
#ClustersOfExcellence #SCALEcluster 🧵
Reposted by Oliver Thon
Solène’s 4.5 years of postdoctoral work in our lab (she started just before the corona pandemic) has now been published. A heroic effort that revealed -at the single molecule level- that ATP drives the dissociation of an ECF transporter complex under turnover conditions. See rdcu.be/elTKc
Single-molecule visualization of ATP-induced dynamics of the subunit composition of an ECF transporter complex under turnover conditions
Nature Communications - The association and dissociation dynamics of the ECF transporter complex for vitamin B12 are visualized by single-molecule FRET, highlighting the original transport...
rdcu.be
May 13, 2025 at 7:30 PM
Solène’s 4.5 years of postdoctoral work in our lab (she started just before the corona pandemic) has now been published. A heroic effort that revealed -at the single molecule level- that ATP drives the dissociation of an ECF transporter complex under turnover conditions. See rdcu.be/elTKc
Reposted by Oliver Thon
This took us a while and I am all the happier it is finally out! I thought that simply adding ATP to purified potassium channel KtrAB should be sufficient to stabilize its active state. I was so wrong...
Read the full story on how an IDR facilitates channel opening upon membrane interaction.
Read the full story on how an IDR facilitates channel opening upon membrane interaction.
A short intrinsically disordered region at KtrB’s N-terminus facilitates allosteric regulation of K+ channel KtrAB
Nature Communications - KtrAB is a major potassium uptake system that has been linked to the pathogenesis of many infectious bacteria. Here the authors show that KtrB from Vibrio alginolyticus...
www.nature.com
May 12, 2025 at 8:51 PM
This took us a while and I am all the happier it is finally out! I thought that simply adding ATP to purified potassium channel KtrAB should be sufficient to stabilize its active state. I was so wrong...
Read the full story on how an IDR facilitates channel opening upon membrane interaction.
Read the full story on how an IDR facilitates channel opening upon membrane interaction.
Reposted by Oliver Thon
Finally, it's out in Nat Commun! Excellent collaboration with my colleagues and friends in Frankfurt, especially @ihaenelt.bsky.social and Janet Vonck.
This work demonstrates the role of the intrinsically disordered N-terminus of KtrAB in channel opening.
Full story here: rdcu.be/elDYO
This work demonstrates the role of the intrinsically disordered N-terminus of KtrAB in channel opening.
Full story here: rdcu.be/elDYO
A short intrinsically disordered region at KtrB’s N-terminus facilitates allosteric regulation of K+ channel KtrAB
Nature Communications - KtrAB is a major potassium uptake system that has been linked to the pathogenesis of many infectious bacteria. Here the authors show that KtrB from Vibrio alginolyticus...
rdcu.be
May 12, 2025 at 9:34 AM
Finally, it's out in Nat Commun! Excellent collaboration with my colleagues and friends in Frankfurt, especially @ihaenelt.bsky.social and Janet Vonck.
This work demonstrates the role of the intrinsically disordered N-terminus of KtrAB in channel opening.
Full story here: rdcu.be/elDYO
This work demonstrates the role of the intrinsically disordered N-terminus of KtrAB in channel opening.
Full story here: rdcu.be/elDYO
Reposted by Oliver Thon
Ball-and-chain inactivation of a human large conductance calcium-activated potassium channel pubmed.ncbi.nlm.nih.gov/39971906/ #cryoem
February 20, 2025 at 10:28 AM
Ball-and-chain inactivation of a human large conductance calcium-activated potassium channel pubmed.ncbi.nlm.nih.gov/39971906/ #cryoem
Reposted by Oliver Thon
In a great collaboration with @hummerlab.bsky.social and the Kräusslich lab: HIV capsid doesn't break at the NPC; instead, it cracks open the NPC itself! Details in Cell: authors.elsevier.com/sd/article/S... @mpibp.bsky.social @uniheidelberg.bsky.social A thread below:
January 17, 2025 at 6:43 PM
In a great collaboration with @hummerlab.bsky.social and the Kräusslich lab: HIV capsid doesn't break at the NPC; instead, it cracks open the NPC itself! Details in Cell: authors.elsevier.com/sd/article/S... @mpibp.bsky.social @uniheidelberg.bsky.social A thread below:
Reposted by Oliver Thon
Take a look at our paper from last week where we probed the Molecular Architecture of Synaptic Vesicles by cryo-ET and integrative structural analysis
We found that V-ATPases interact with synaptophysin and describe some other molecules of this exciting organelle
🧠🔬❄️
www.pnas.org/doi/10.1073/...
We found that V-ATPases interact with synaptophysin and describe some other molecules of this exciting organelle
🧠🔬❄️
www.pnas.org/doi/10.1073/...
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
www.pnas.org
December 3, 2024 at 9:53 AM
Take a look at our paper from last week where we probed the Molecular Architecture of Synaptic Vesicles by cryo-ET and integrative structural analysis
We found that V-ATPases interact with synaptophysin and describe some other molecules of this exciting organelle
🧠🔬❄️
www.pnas.org/doi/10.1073/...
We found that V-ATPases interact with synaptophysin and describe some other molecules of this exciting organelle
🧠🔬❄️
www.pnas.org/doi/10.1073/...