S Saif Hasan
@ssaifhasan.bsky.social
Basketball enthusiast, foodie, and assistant professor
Hello Colleagues. We are purifying a complex of two #membrane proteins from Expi293 cells. We used 1% DDM for solubilization, and 0.02% during chromatography. The complex shows both subunits on the gel but runs really small on SEC. It should have 3 copies of one subunit and 2 of the other.
November 20, 2024 at 5:29 PM
Hello Colleagues. We are purifying a complex of two #membrane proteins from Expi293 cells. We used 1% DDM for solubilization, and 0.02% during chromatography. The complex shows both subunits on the gel but runs really small on SEC. It should have 3 copies of one subunit and 2 of the other.
Reposted by S Saif Hasan
November 8, 2024 at 10:01 PM
Hello, I am an Assistant Professor at the Univ of Maryland. We investigate #coronavirus glycoprotein trafficking, using #cryoem #cryoet #xraycrystallography and functional tools. Here is one of our no-longer-recent publications. Great to see a vibrant community!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
A single C-terminal residue controls SARS-CoV-2 spike trafficking and incorporation into VLPs - Nature Communications
Dey et al. use structure-function methods to show that partial mimicry of the coatomer-binding motif in the SARS-CoV-2 spike is crucial for its release post coatomer-dependent delivery, thus ensuring ...
www.nature.com
November 13, 2024 at 12:30 PM
Hello, I am an Assistant Professor at the Univ of Maryland. We investigate #coronavirus glycoprotein trafficking, using #cryoem #cryoet #xraycrystallography and functional tools. Here is one of our no-longer-recent publications. Great to see a vibrant community!
www.nature.com/articles/s41...
www.nature.com/articles/s41...