Marija Krasiļņikova
@scotomorph.bsky.social
Parasitology @ McCulloch lab at the University of Glasgow
Pinned
Nanopore sequencing reveals that DNA replication compartmentalisation dictates genome stability and instability in Trypanosoma brucei - Nature Communications
A full description of trypanosome genome replication has proved elusive. Here, after genome assembly, the authors reveal compartmentalisation of DNA replication activity and stability between transcri...
doi.org
DNA replication beyond the cores of megabase chromosomes of T. brucei -- some of my PhD work is FINALLY out! doi.org/10.1038/s414... thank you to @rmc9z.bsky.social @marquescata.bsky.social Emma, Dario, Kathryn, Graham and Craig!
Reposted by Marija Krasiļņikova
Variation in surface protein expression leads to heterogeneous Trypanosoma cruzi populations during host cell infection pubmed.ncbi.nlm.nih.gov/41224774/
November 13, 2025 at 8:15 AM
Variation in surface protein expression leads to heterogeneous Trypanosoma cruzi populations during host cell infection pubmed.ncbi.nlm.nih.gov/41224774/
Reposted by Marija Krasiļņikova
Trypanosoma brucei cattle infections contain cryptic transmission-adapted bloodstream forms at low parasitaemia pubmed.ncbi.nlm.nih.gov/41193429/
November 6, 2025 at 10:02 AM
Trypanosoma brucei cattle infections contain cryptic transmission-adapted bloodstream forms at low parasitaemia pubmed.ncbi.nlm.nih.gov/41193429/
Reposted by Marija Krasiļņikova
The evolutionary origin of host association and polycistronic transcription in trypanosomatids www.biorxiv.org/content/10.1101/2025.10.27.684775v1
October 28, 2025 at 7:15 AM
The evolutionary origin of host association and polycistronic transcription in trypanosomatids www.biorxiv.org/content/10.1101/2025.10.27.684775v1
Reposted by Marija Krasiļņikova
academic.oup.com/nar/article/...
Great to see this out! Big shout out to Douglas Escrivani, Seb Hutchinson & the Horn Lab!
@dundee.ac.uk
@ybri-uoy.bsky.social @york.ac.uk
Great to see this out! Big shout out to Douglas Escrivani, Seb Hutchinson & the Horn Lab!
@dundee.ac.uk
@ybri-uoy.bsky.social @york.ac.uk
A non-coding role for trypanosome VSG transcripts in allelic exclusion
Abstract. Bloodstream-form African trypanosomes display antigenic variation. This requires mono-telomeric but switchable expression of a Variant Surface Gl
academic.oup.com
October 22, 2025 at 9:35 AM
academic.oup.com/nar/article/...
Great to see this out! Big shout out to Douglas Escrivani, Seb Hutchinson & the Horn Lab!
@dundee.ac.uk
@ybri-uoy.bsky.social @york.ac.uk
Great to see this out! Big shout out to Douglas Escrivani, Seb Hutchinson & the Horn Lab!
@dundee.ac.uk
@ybri-uoy.bsky.social @york.ac.uk
Reposted by Marija Krasiļņikova
Our lab has two funded PhDs to start in 2026 via the NorthWestBio programme
Project details here
www.gla.ac.uk/postgraduate...
...and here
www.gla.ac.uk/postgraduate...
Application deadline is Nov 21st 2025; apply here:
www.gla.ac.uk/postgraduate...
Project details here
www.gla.ac.uk/postgraduate...
...and here
www.gla.ac.uk/postgraduate...
Application deadline is Nov 21st 2025; apply here:
www.gla.ac.uk/postgraduate...
University of Glasgow - Postgraduate study - Centres for Doctoral Training - NorthWest Biosciences - Our Projects - Understanding Pathogens, from Molecules to Phenotypes - Richard McCulloch
www.gla.ac.uk
October 6, 2025 at 1:23 PM
Our lab has two funded PhDs to start in 2026 via the NorthWestBio programme
Project details here
www.gla.ac.uk/postgraduate...
...and here
www.gla.ac.uk/postgraduate...
Application deadline is Nov 21st 2025; apply here:
www.gla.ac.uk/postgraduate...
Project details here
www.gla.ac.uk/postgraduate...
...and here
www.gla.ac.uk/postgraduate...
Application deadline is Nov 21st 2025; apply here:
www.gla.ac.uk/postgraduate...
Reposted by Marija Krasiļņikova
Targets of the human antibody response to the variant surface glycoprotein of Trypanosoma brucei pubmed.ncbi.nlm.nih.gov/41040341/
October 4, 2025 at 12:12 PM
Targets of the human antibody response to the variant surface glycoprotein of Trypanosoma brucei pubmed.ncbi.nlm.nih.gov/41040341/
Reposted by Marija Krasiļņikova
The UTRs of Leishmania donovani vary in length and are enriched in potential regulatory structures pubmed.ncbi.nlm.nih.gov/41040140/
October 4, 2025 at 3:29 PM
The UTRs of Leishmania donovani vary in length and are enriched in potential regulatory structures pubmed.ncbi.nlm.nih.gov/41040140/
Reposted by Marija Krasiļņikova
Phylogenomics of free-living neobodonids reveals they are a paraphyletic group from which all other metakinetoplastids are descended www.biorxiv.org/content/10.1101/2025.10.03.680260v1
October 5, 2025 at 7:16 AM
Phylogenomics of free-living neobodonids reveals they are a paraphyletic group from which all other metakinetoplastids are descended www.biorxiv.org/content/10.1101/2025.10.03.680260v1
Reposted by Marija Krasiļņikova
Splice site diversity and abundance of non-canonical introns in diplonemids (Diplonemea, Euglenozoa) pubmed.ncbi.nlm.nih.gov/41027712/
October 1, 2025 at 6:05 PM
Splice site diversity and abundance of non-canonical introns in diplonemids (Diplonemea, Euglenozoa) pubmed.ncbi.nlm.nih.gov/41027712/
Reposted by Marija Krasiļņikova
First Genome-Wide Centromere Map of Trypanosoma cruzi Reveals Linear and 3D Compartment Boundaries and Spatial Clustering www.biorxiv.org/content/10.1101/2025.09.26.678825v1
September 30, 2025 at 7:16 AM
First Genome-Wide Centromere Map of Trypanosoma cruzi Reveals Linear and 3D Compartment Boundaries and Spatial Clustering www.biorxiv.org/content/10.1101/2025.09.26.678825v1
Reposted by Marija Krasiļņikova
Trypanosome histone variants H3.V and H4.V promote nucleosome plasticity in repressed chromatin. www.biorxiv.org/content/10.1101/2025.09.24.678203v1
September 27, 2025 at 7:15 AM
Trypanosome histone variants H3.V and H4.V promote nucleosome plasticity in repressed chromatin. www.biorxiv.org/content/10.1101/2025.09.24.678203v1
Reposted by Marija Krasiļņikova
The mitotic spindle kinase MSK co-ordinates segregation of the nucleus and kinetoplast in Leishmania mexicana www.biorxiv.org/content/10.1101/2025.09.19.677349v1
September 22, 2025 at 7:16 AM
The mitotic spindle kinase MSK co-ordinates segregation of the nucleus and kinetoplast in Leishmania mexicana www.biorxiv.org/content/10.1101/2025.09.19.677349v1
New pre-print from Guy @goldrieve.bsky.social on vsgseq2 - a much improved, robust tool to analyse VSG expression in tryps
vsgseq2: an updated pipeline for analysis of the diversity and abundance of population-wide Trypanosoma brucei VSG expression www.biorxiv.org/content/10.1101/2025.09.12.675796v1
September 18, 2025 at 1:24 PM
New pre-print from Guy @goldrieve.bsky.social on vsgseq2 - a much improved, robust tool to analyse VSG expression in tryps
Reposted by Marija Krasiļņikova
vsgseq2: an updated pipeline for analysis of the diversity and abundance of population-wide Trypanosoma brucei VSG expression www.biorxiv.org/content/10.1101/2025.09.12.675796v1
September 18, 2025 at 7:16 AM
vsgseq2: an updated pipeline for analysis of the diversity and abundance of population-wide Trypanosoma brucei VSG expression www.biorxiv.org/content/10.1101/2025.09.12.675796v1
Reposted by Marija Krasiļņikova
Chemical genetics reveals Leishmania KKT2 and CRK9 kinase activity is required for cell cycle progression www.biorxiv.org/content/10.1101/2025.09.08.674917v1
September 13, 2025 at 7:16 AM
Chemical genetics reveals Leishmania KKT2 and CRK9 kinase activity is required for cell cycle progression www.biorxiv.org/content/10.1101/2025.09.08.674917v1
Reposted by Marija Krasiļņikova
Save the dates!! @bspparasitology.bsky.social Spring Meeting 2026 will be in Bonnie Glasgow! 7-9 April 2026, with a drinks reception in Glasgow City Chambers on the 6th of April 2026!
Keep an eye on the BSP website for registration and abstract submission!
Logo by @shannaraeilean.bsky.social! 🦟
Keep an eye on the BSP website for registration and abstract submission!
Logo by @shannaraeilean.bsky.social! 🦟
September 11, 2025 at 11:09 AM
Save the dates!! @bspparasitology.bsky.social Spring Meeting 2026 will be in Bonnie Glasgow! 7-9 April 2026, with a drinks reception in Glasgow City Chambers on the 6th of April 2026!
Keep an eye on the BSP website for registration and abstract submission!
Logo by @shannaraeilean.bsky.social! 🦟
Keep an eye on the BSP website for registration and abstract submission!
Logo by @shannaraeilean.bsky.social! 🦟
Reposted by Marija Krasiļņikova
A novel high-throughput single-cell DNA sequencing method reveals hidden genomic heterogeneity in the unicellular eukaryote Leishmania www.biorxiv.org/content/10.1101/2025.09.10.675331v1
September 11, 2025 at 7:16 AM
A novel high-throughput single-cell DNA sequencing method reveals hidden genomic heterogeneity in the unicellular eukaryote Leishmania www.biorxiv.org/content/10.1101/2025.09.10.675331v1
Reposted by Marija Krasiļņikova
Transcriptomic and proteomic analysis of quiescent epimastigotes as a resource for investigating Trypanosoma cruzi persistence. www.biorxiv.org/content/10.1101/2025.09.09.675056v1
September 10, 2025 at 7:16 AM
Transcriptomic and proteomic analysis of quiescent epimastigotes as a resource for investigating Trypanosoma cruzi persistence. www.biorxiv.org/content/10.1101/2025.09.09.675056v1
Reposted by Marija Krasiļņikova
Genome-wide overexpression screen reveals a novel transcription termination factor in Trypanosoma brucei www.biorxiv.org/content/10.1101/2025.09.02.673782v1
September 6, 2025 at 7:16 AM
Genome-wide overexpression screen reveals a novel transcription termination factor in Trypanosoma brucei www.biorxiv.org/content/10.1101/2025.09.02.673782v1
Reposted by Marija Krasiļņikova
A Trypanosome Trifecta: an independently tunable triple inducer system for genetic studies in Trypanosoma brucei. www.biorxiv.org/content/10.1101/2025.08.31.673413v1
September 2, 2025 at 7:16 AM
A Trypanosome Trifecta: an independently tunable triple inducer system for genetic studies in Trypanosoma brucei. www.biorxiv.org/content/10.1101/2025.08.31.673413v1
Reposted by Marija Krasiļņikova
How do parasites strategically organise their genomes to influence gene expression? If you’re curious, check our latest review in @cp-trendsparasitol.bsky.social !
@biologyatyork.bsky.social @ybri-uoy.bsky.social @pasteur.fr
www.sciencedirect.com/science/arti...
@biologyatyork.bsky.social @ybri-uoy.bsky.social @pasteur.fr
www.sciencedirect.com/science/arti...
August 11, 2025 at 6:40 AM
How do parasites strategically organise their genomes to influence gene expression? If you’re curious, check our latest review in @cp-trendsparasitol.bsky.social !
@biologyatyork.bsky.social @ybri-uoy.bsky.social @pasteur.fr
www.sciencedirect.com/science/arti...
@biologyatyork.bsky.social @ybri-uoy.bsky.social @pasteur.fr
www.sciencedirect.com/science/arti...
Reposted by Marija Krasiļņikova
Single-molecule landscape of DNA replication pausing https://www.biorxiv.org/content/10.1101/2025.08.14.670160v1
August 15, 2025 at 3:34 AM
Single-molecule landscape of DNA replication pausing https://www.biorxiv.org/content/10.1101/2025.08.14.670160v1
Reposted by Marija Krasiļņikova
The absence of the Leishmania major telomerase TERT component links telomeres and cell homeostasis with infectivity pubmed.ncbi.nlm.nih.gov/40848782/
August 24, 2025 at 5:29 PM
The absence of the Leishmania major telomerase TERT component links telomeres and cell homeostasis with infectivity pubmed.ncbi.nlm.nih.gov/40848782/
Reposted by Marija Krasiļņikova
We’re pleased to share our new study, led by @jezieldamasceno.bsky.social, that used long-read, single molecule sequencing to reveal the full (?) picture of Leishmania DNA replication. Thanks, Mike Boemo and helpful reviewers @uofgsii.bsky.social
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Leishmania major chromosomes are replicated from a single high-efficiency locus supplemented by thousands of lower efficiency initiation events
DNA replication initiates at genome sites termed origins. Previous origin mapping approaches in the populations of the protozoan parasite Leishmania m…
www.sciencedirect.com
August 15, 2025 at 7:32 AM
We’re pleased to share our new study, led by @jezieldamasceno.bsky.social, that used long-read, single molecule sequencing to reveal the full (?) picture of Leishmania DNA replication. Thanks, Mike Boemo and helpful reviewers @uofgsii.bsky.social
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Reposted by Marija Krasiļņikova
Integrating tRNA gene epigenomics and expression with codon usage unravels an intricate connection with translatome dynamics in Trypanosoma cruzi pubmed.ncbi.nlm.nih.gov/40788054/
August 12, 2025 at 9:49 AM
Integrating tRNA gene epigenomics and expression with codon usage unravels an intricate connection with translatome dynamics in Trypanosoma cruzi pubmed.ncbi.nlm.nih.gov/40788054/