Code script
@nonstandardcode.bsky.social
Reposted by Code script
Our one-pot biocatalytic cascade for synthesis of Phe derivatives is now available online at ChemCatChem
chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
Also online now on bioRxiv is our larger story where we couple this pathway to GCE tools in live cells
www.biorxiv.org/content/10.1...
chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
Also online now on bioRxiv is our larger story where we couple this pathway to GCE tools in live cells
www.biorxiv.org/content/10.1...
December 23, 2024 at 9:45 PM
Our one-pot biocatalytic cascade for synthesis of Phe derivatives is now available online at ChemCatChem
chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
Also online now on bioRxiv is our larger story where we couple this pathway to GCE tools in live cells
www.biorxiv.org/content/10.1...
chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/...
Also online now on bioRxiv is our larger story where we couple this pathway to GCE tools in live cells
www.biorxiv.org/content/10.1...
Automated o-tRNA generation: from the Jason Chin group
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Automated orthogonal tRNA generation - Nature Chemical Biology
Genetic code expansion and reprogramming require orthogonal tRNAs. Methods have now been developed for the automated generation of chimeric orthogonal tRNAs and discovery of their cognate synthetases....
www.nature.com
December 20, 2024 at 1:41 PM
Automated o-tRNA generation: from the Jason Chin group
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Reposted by Code script
Engineering a ribozyme with aminoacyl-tRNA synthetase activity https://www.biorxiv.org/content/10.1101/2024.12.12.627959v1
December 13, 2024 at 3:46 AM
Engineering a ribozyme with aminoacyl-tRNA synthetase activity https://www.biorxiv.org/content/10.1101/2024.12.12.627959v1
Reposted by Code script
Check out our latest pre-print on building biocatalytic cascades for the synthesis of phenylalanine derivatives for applications in genetic code expansion
www.biorxiv.org/content/10.1...
Andrew Buller's lab at Wisconsin is also doing terrific work in this area - stay tuned for that too
#synbio
www.biorxiv.org/content/10.1...
Andrew Buller's lab at Wisconsin is also doing terrific work in this area - stay tuned for that too
#synbio
A One-Pot Biocatalytic Cascade to Access Diverse L-Phenylalanine Derivatives from Aldehydes or Carboxylic Acids
Non-standard amino acids (nsAAs) that are L-phenylalanine derivatives with aryl ring functionalization have long been harnessed in natural product synthesis, therapeutic peptide synthesis, and diverse...
www.biorxiv.org
December 11, 2024 at 4:31 AM
Check out our latest pre-print on building biocatalytic cascades for the synthesis of phenylalanine derivatives for applications in genetic code expansion
www.biorxiv.org/content/10.1...
Andrew Buller's lab at Wisconsin is also doing terrific work in this area - stay tuned for that too
#synbio
www.biorxiv.org/content/10.1...
Andrew Buller's lab at Wisconsin is also doing terrific work in this area - stay tuned for that too
#synbio
Reposted by Code script
Check out 🏆A Biologist’s Guide to Mars Dirt, where we present a receipt for soluble Mars Regolith Media (sMRM) that best simulates what you’d get if you added water to Mars dirt.
Will anything grow in this media? Stay tuned!
💧+ 🪨 → 🌱 ?
open.substack.com/pub/pioneerl...
Will anything grow in this media? Stay tuned!
💧+ 🪨 → 🌱 ?
open.substack.com/pub/pioneerl...
🏆 A Biologist’s Guide to Mars Dirt
Just add water
open.substack.com
December 10, 2024 at 5:27 PM
Check out 🏆A Biologist’s Guide to Mars Dirt, where we present a receipt for soluble Mars Regolith Media (sMRM) that best simulates what you’d get if you added water to Mars dirt.
Will anything grow in this media? Stay tuned!
💧+ 🪨 → 🌱 ?
open.substack.com/pub/pioneerl...
Will anything grow in this media? Stay tuned!
💧+ 🪨 → 🌱 ?
open.substack.com/pub/pioneerl...
Reposted by Code script
Nice work from Tao Liu lab on engineering novel fluorescent proteins using a rotor-based fluorogenic noncanonical amino acid www.nature.com/articles/s41...
Designing artificial fluorescent proteins and biosensors by genetically encoding molecular rotor-based amino acids - Nature Chemistry
The toolbox of artificial fluorescent proteins can be expanded by engineering mimics of the molecular rotor-based fluorophore found in the green fluorescent protein (GFP) into diverse protein scaffold...
www.nature.com
November 30, 2024 at 1:47 PM
Nice work from Tao Liu lab on engineering novel fluorescent proteins using a rotor-based fluorogenic noncanonical amino acid www.nature.com/articles/s41...
Reposted by Code script
Happy to share Elise and Tarah’s work on building a facile selection system to engineer E. coli leucyl synthetase for site-specific incorporation many new noncanonical amino acids in eukaryotes, including the novel post-translational modification N-acetyl-methyllysine
Optimized directed evolution of E. coli leucyl-tRNA synthetase adds many noncanonical amino acids into the eukaryotic genetic code including ornithine and Nepsilon-acetyl-methyllysine https://www.biorxiv.org/content/10.1101/2024.11.27.625662v1
Optimized directed evolution of E. coli leucyl-tRNA synthetase adds many noncanonical amino acids into the eukaryotic genetic code including ornithine and Nepsilon-acetyl-methyllysine https://www.biorxiv.org/content/10.1101/2024.11.27.625662v1
Site-specific incorporation of noncanonical amino acids (ncAAs) into proteins in eukaryotes has pred
www.biorxiv.org
November 28, 2024 at 4:40 AM
Happy to share Elise and Tarah’s work on building a facile selection system to engineer E. coli leucyl synthetase for site-specific incorporation many new noncanonical amino acids in eukaryotes, including the novel post-translational modification N-acetyl-methyllysine
Reposted by Code script
Sustainable Regeneration of 20 Aminoacyl-tRNA Synthetases in a Reconstituted System Toward Self-Synthesizing Artificial Systems https://www.biorxiv.org/content/10.1101/2024.10.03.616507v1
Sustainable Regeneration of 20 Aminoacyl-tRNA Synthetases in a Reconstituted System Toward Self-Synthesizing Artificial Systems https://www.biorxiv.org/content/10.1101/2024.10.03.616507v1
In vitro construction of self-reproducible artificial systems is a major challenge in bottom-up synt
www.biorxiv.org
October 4, 2024 at 6:03 AM
Sustainable Regeneration of 20 Aminoacyl-tRNA Synthetases in a Reconstituted System Toward Self-Synthesizing Artificial Systems https://www.biorxiv.org/content/10.1101/2024.10.03.616507v1
Reposted by Code script
Here for my first post. Our new approach to study tRNA aminoacylation by nanopore sequencing. Started as a napkin sketch ~1 year ago! This was a fun one, congrats to @laurakwhite.bsky.social and Aleks.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Nanopore sequencing of intact aminoacylated tRNAs
Transfer RNAs (tRNA) are decorated during biogenesis with a variety of modifications that modulate their stability, aminoacylation, and decoding potential during translation. The complex landscape of ...
www.biorxiv.org
November 19, 2024 at 3:07 AM
Here for my first post. Our new approach to study tRNA aminoacylation by nanopore sequencing. Started as a napkin sketch ~1 year ago! This was a fun one, congrats to @laurakwhite.bsky.social and Aleks.
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Reposted by Code script
We added another 100 citations (total 577) in the latest version of our review on LLMs and agents in chemistry. Take a look!
arxiv.org/abs/2407.01603
arxiv.org/abs/2407.01603
November 18, 2024 at 2:05 AM
We added another 100 citations (total 577) in the latest version of our review on LLMs and agents in chemistry. Take a look!
arxiv.org/abs/2407.01603
arxiv.org/abs/2407.01603
Reposted by Code script
Computational Design of Metallohydrolases https://www.biorxiv.org/content/10.1101/2024.11.13.623507v1
Computational Design of Metallohydrolases https://www.biorxiv.org/content/10.1101/2024.11.13.623507v1
New enzymes can be designed by starting from a description of an ideal active site composed of catal
www.biorxiv.org
November 15, 2024 at 1:45 AM
Computational Design of Metallohydrolases https://www.biorxiv.org/content/10.1101/2024.11.13.623507v1