Montse Grifé
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montsegrife.bsky.social
Montse Grifé
@montsegrife.bsky.social
PhD candidate interested in bacterial biocontrol agents and molecular mechanisms underlying phytopathogen antagonism and plant-growth promotion🌿🦠🧬
https://www.linkedin.com/in/montserrat-grif%C3%A9-ruiz-106709144?lipi=urn%3Ali%3Apage%3Ad_flagship3_prof
Reposted by Montse Grifé
Happy to share our paper from a new research line led by @camolsan.bsky.social, where we engineered the T6SS of Pseudomonas putida to deliver effectors against bacterial competitors and fungal pathogens like Botrytis cinerea. @ihsmumacsic.bsky.social

jbioleng.biomedcentral.com/articles/10....
Engineering the T6SS of Pseudomonas for targeted delivery of antibacterial and antifungal effectors - Journal of Biological Engineering
Background Bacteria employ diverse molecular systems, such as the type VI secretion system (T6SS) to outcompete other microorganisms and adapt to ecological niches. The T6SS is a versatile nanomachine...
jbioleng.biomedcentral.com
April 3, 2025 at 6:00 PM
Reposted by Montse Grifé
Excited to share our latest preprint! A fascinating story of chemical dialogue and microbial warfare: the Bacillus extracellular matrix acts as a key driver in the antagonistic interaction with Botrytis, while the fungus mounts a multi-layered defense.
🔗

www.biorxiv.org/content/10.1...
The offensive role of the Bacillus extracellular matrix in driving metabolite-mediated dialogue and adaptive strategies with pathogenic fungi
Bacterial□fungal interactions have traditionally been attributed to secondary metabolites, but the role of the bacterial extracellular matrix (ECM) in shaping these relationships has remained unclear....
www.biorxiv.org
February 11, 2025 at 8:01 AM
Reposted by Montse Grifé
Really cool #singlecell method published in Science! Multiplexed error-robust fluorescence in situ hybridization (MERFISH) to create an image-based single-cell transcriptomics in bacteria 🦠🧫🔬🧪
🅲🅾🅾🅻 🆃🅴🅲🅷🅽🅾🅻🅾🅶🆈

“Bacterial-MERFISH” provides ~1000-fold volumetric expansion of individual cells, charts gene expression in hundreds of thousands of cells, deciphering bacterial single-cell heterogeneity, intracellular transcriptome organization, and bacterial adaptation to µm-scale niches in vivo
Highly multiplexed spatial transcriptomics in bacteria
Single-cell decisions made in complex environments underlie many bacterial phenomena. Image-based transcriptomics approaches offer an avenue to study such behaviors, yet these approaches have been hin...
www.science.org
January 25, 2025 at 5:58 PM
Super happy to share my first article at @diegromero.bsky.social´s lab, about Bacillus cyclic lipopeptides and how the diversification of structural variants production impacts biological activities!!
pubs.acs.org/doi/10.1021/...
Diversification of Lipopeptide Analogues Drives Versatility in Biological Activities
Cyclic lipopeptides (CLPs) are potent secondary metabolites with diverse biological functions. Bacillus strains primarily produce CLPs of three key families, namely, iturins, fengycins, and surfactins...
pubs.acs.org
January 25, 2025 at 1:58 PM