🧬scCRISPRi screening of 65 Schizophrenia-linked TFs/ERs in iPSC NPCs/neurons to reveal their impact on gene regulatory mechanisms of neurodev!

Happy to been part of the single cell tech dev/analysis of this study lead by @umut_yildiz12 from EMBL/Noh lab! www.biorxiv.org/content/10.1...

SUM-seq is an ultra-high-throughput method for co-profiling chromatin accessibility and gene expression in single nuclei. @anniquec.bsky.social

www.nature.com/articles/s41...

SUM-seq: New single-cell method by @biomedizin.unibas.ch , @embl.org , @au.dk ‬‬ and Tampere university enables large-scale analysis of gene activity & DNA accessibility across millions of cells.
Congrats Prof. Zaugg & collaborators!‬‬‬‬‬‬

🔗 www.nature.com/articles/s41...

SUM-seq out @natmethods.nature.com !


🚀 Ultra-high-throughput Multiplexed snATAC+RNA

Used to:
⏳ link temporal macrophage GRNs to immune disease genetics
🩸 map T cell regulatory landscapes
🧬✂️ dissect TF function in hiPSC differentiation via CRISPRi/a screens
doi.org/10.1038/s41592-025-02700-8
🧵

1/🚀 Excited to share RegVelo, our new cell model combining RNA velocity with gene regulatory network (GRN) dynamics to model cellular changes and predict in silico perturbations. Here's how it works and why it matters! 🧵👇
biorxiv.org/content/10.1101/2024.12.11.627935v1

Right before the end of the year the Zaugg and Noh teams at EMBL shared SUM-seq: a scalable single cell ATAC+RNA method.

Perfect if you want to scale up time course, drug/CRISPR screen or atlas projects! 🖥️ 🧬
www.biorxiv.org/content/10.1...