Mitchell O'Connell
@mitchelloconnell.bsky.social
Associate Professor, Biochemistry and Biophysics,
University of Rochester. RNA-targeting CRISPR / CRISPR membrane proteins / RNA acetylation. An Aussie in 'the land of the free'. Music Nerd
University of Rochester. RNA-targeting CRISPR / CRISPR membrane proteins / RNA acetylation. An Aussie in 'the land of the free'. Music Nerd
My lab was stoked to play a role in this fun collaboration with
@doc-jlmeier.bsky.social , Colin Wu and Schraga Schwartz labs among others, where we try to figure out the role of ac4C tRNA mods in mammalian stress signaling. See below for an excellent summary from Jordan (thanks!)
@doc-jlmeier.bsky.social , Colin Wu and Schraga Schwartz labs among others, where we try to figure out the role of ac4C tRNA mods in mammalian stress signaling. See below for an excellent summary from Jordan (thanks!)
Excited to share this one! We developed an in vivo model for specific manipulation of transfer RNA acetylation and found it serves as a sentinel modification whose loss causes ribosome stalling and stress signaling. Implications for a genetic disorder and cancer.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Transfer RNA acetylation regulates in vivo mammalian stress signaling
An ancient tRNA modification is used by mammalian cells to coordinate protein translation and adaptive signaling.
www.science.org
March 21, 2025 at 3:22 PM
My lab was stoked to play a role in this fun collaboration with
@doc-jlmeier.bsky.social , Colin Wu and Schraga Schwartz labs among others, where we try to figure out the role of ac4C tRNA mods in mammalian stress signaling. See below for an excellent summary from Jordan (thanks!)
@doc-jlmeier.bsky.social , Colin Wu and Schraga Schwartz labs among others, where we try to figure out the role of ac4C tRNA mods in mammalian stress signaling. See below for an excellent summary from Jordan (thanks!)
Reposted by Mitchell O'Connell
Session 9: Mitch O'Connell bacterial/bacteriophage arms race. Small RNAs to mount an adaptive MGE immune response. CRISPR RNA defence systems. How does Cas13 work as an enzyme to target RNA? Csx28 128 aa protein, TM, cyt. Co-opt membrane effectors? 👏 #LorneProteins2025
February 11, 2025 at 11:25 PM
Session 9: Mitch O'Connell bacterial/bacteriophage arms race. Small RNAs to mount an adaptive MGE immune response. CRISPR RNA defence systems. How does Cas13 work as an enzyme to target RNA? Csx28 128 aa protein, TM, cyt. Co-opt membrane effectors? 👏 #LorneProteins2025
Preprint alert! Brandon a shared student between my lab and @pjmurphy249.bsky.social 's has uncovered some biases in ChIP-seq that means we may be missing signal in heterochromatic regions of the genome, and tagmentation based techniques can overcome this. See below for Patrick's fantastic summary:
PREPRINT! Park et al. makes the case that we may be misunderstanding heterochromatin for past 30 years due to ChIP-Seq biases... 1/n
CUT&Tag Identifies Repetitive Genomic Loci that are Excluded from ChIP Assays https://www.biorxiv.org/content/10.1101/2025.02.03.636299v1
February 5, 2025 at 9:35 PM
Preprint alert! Brandon a shared student between my lab and @pjmurphy249.bsky.social 's has uncovered some biases in ChIP-seq that means we may be missing signal in heterochromatic regions of the genome, and tagmentation based techniques can overcome this. See below for Patrick's fantastic summary: