LolandLab
@lolandlab.bsky.social
Structure-function relationship on transport proteins. Fluorescence, cryo-EM and molecular pharmacology on neurotransmitter:sodium symporters.
Pinned
LolandLab
@lolandlab.bsky.social
· Nov 23
Hi all transporter aficionados. Join our starter pack on membrane transport proteins. Pumps, secondary active transport or exchangers. Everything concerning these fascinating molecular motors are welcomed here.
go.bsky.app/58qb8h5
go.bsky.app/58qb8h5
Looking for a Post doc position in Structural Biology? We are hiring!
See post here:
jobbank.dk/job/2850337/...
The task is to continue our endeavor in solving cryo-EM structures of the human dopamine transporter. See: doi.org/10.1038/s415...
Thanks to the Novo Nordisk Foundation for funding.
See post here:
jobbank.dk/job/2850337/...
The task is to continue our endeavor in solving cryo-EM structures of the human dopamine transporter. See: doi.org/10.1038/s415...
Thanks to the Novo Nordisk Foundation for funding.
Faculty and academic positions – University of Copenhagen
https://employment.ku.dk/faculty/?show=…
July 8, 2025 at 9:45 AM
Looking for a Post doc position in Structural Biology? We are hiring!
See post here:
jobbank.dk/job/2850337/...
The task is to continue our endeavor in solving cryo-EM structures of the human dopamine transporter. See: doi.org/10.1038/s415...
Thanks to the Novo Nordisk Foundation for funding.
See post here:
jobbank.dk/job/2850337/...
The task is to continue our endeavor in solving cryo-EM structures of the human dopamine transporter. See: doi.org/10.1038/s415...
Thanks to the Novo Nordisk Foundation for funding.
A huge congrats to Jeppe Nielsen who defended his PhD thesis with excellence! An outstanding accomplishment! Thanks to profs. Harald Sitte, @poulnissen.bsky.social and Petrine Wellendorph for an inspiring assessment.
June 8, 2025 at 5:53 AM
A huge congrats to Jeppe Nielsen who defended his PhD thesis with excellence! An outstanding accomplishment! Thanks to profs. Harald Sitte, @poulnissen.bsky.social and Petrine Wellendorph for an inspiring assessment.
📢 ONE MONTH TO GO: Chemical Solutions to Structural Biology Symposium!
Join us at CSSB 2025, a premier two-day international symposium at UCPH exploring groundbreaking chemical and structural biology solutions.
📅 June 12-13, 2025
Registration closes May 30 🔗 isbuc.ku.dk/chemical-sol...
Join us at CSSB 2025, a premier two-day international symposium at UCPH exploring groundbreaking chemical and structural biology solutions.
📅 June 12-13, 2025
Registration closes May 30 🔗 isbuc.ku.dk/chemical-sol...
May 14, 2025 at 7:33 PM
📢 ONE MONTH TO GO: Chemical Solutions to Structural Biology Symposium!
Join us at CSSB 2025, a premier two-day international symposium at UCPH exploring groundbreaking chemical and structural biology solutions.
📅 June 12-13, 2025
Registration closes May 30 🔗 isbuc.ku.dk/chemical-sol...
Join us at CSSB 2025, a premier two-day international symposium at UCPH exploring groundbreaking chemical and structural biology solutions.
📅 June 12-13, 2025
Registration closes May 30 🔗 isbuc.ku.dk/chemical-sol...
A shout out to Kristine Salomon, who now can call herself Dr Salomon after a brilliant PhD defense. Having two papers in Nature, one in @pnas.org, one in @naturecomms.bsky.social and two others, must be a pass. So well deserved. Thanks to Profs Ryan, Drew and Strømgaard for assessment.
March 31, 2025 at 8:00 PM
A shout out to Kristine Salomon, who now can call herself Dr Salomon after a brilliant PhD defense. Having two papers in Nature, one in @pnas.org, one in @naturecomms.bsky.social and two others, must be a pass. So well deserved. Thanks to Profs Ryan, Drew and Strømgaard for assessment.
We are organizing this symposium on structural biology in beautiful Copenhagen. Checkout the impressive lineup!
Chemical Solutions to Structural Biology symposium.
Registration and abstract submission now open:
isbuc.ku.dk/chemical-sol...
Registration and abstract submission now open:
isbuc.ku.dk/chemical-sol...
February 12, 2025 at 12:56 PM
We are organizing this symposium on structural biology in beautiful Copenhagen. Checkout the impressive lineup!
@iriskalen.bsky.social defended her PhD with excellence and can now call herself Dr Iris. Thanks to the assessment committee: Simon Newstead, @bjornppedersen.bsky.social and Nanna MacAulay
February 2, 2025 at 1:52 PM
@iriskalen.bsky.social defended her PhD with excellence and can now call herself Dr Iris. Thanks to the assessment committee: Simon Newstead, @bjornppedersen.bsky.social and Nanna MacAulay
Squeeze pumping of lipids and insecticides by ABCH transporter
www.cell.com/cell/abstrac...
www.cell.com/cell/abstrac...
Squeeze pumping of lipids and insecticides by ABCH transporter
High-resolution cryo-EM structures and biochemical data reveal the dynamic process by which the ABCH transporter binds and transports lipid substrates and insecticides across cell membranes while detoxifying insecticides, providing atomic-level insights for the development of arthropod pest control agents and strategies for managing pesticide resistance.
www.cell.com
December 28, 2024 at 11:42 AM
Squeeze pumping of lipids and insecticides by ABCH transporter
www.cell.com/cell/abstrac...
www.cell.com/cell/abstrac...
Trapping of spermine, Kukoamine A and polyamine toxin blockers in GluK2 kainate receptor channels.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Trapping of spermine, Kukoamine A, and polyamine toxin blockers in GluK2 kainate receptor channels - Nature Communications
Kainate receptors (KARs) contribute to excitatory neurotransmission, neuronal plasticity and neurological disorders. Here, Gangwar et al. present KAR structures in complex with channel blockers NpTx8,...
www.nature.com
December 26, 2024 at 12:44 PM
Trapping of spermine, Kukoamine A and polyamine toxin blockers in GluK2 kainate receptor channels.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Molecular insights into the activation mechanism of GPR156 in maintaining auditory function
www.nature.com/articles/s41...
www.nature.com/articles/s41...
December 15, 2024 at 11:43 AM
Molecular insights into the activation mechanism of GPR156 in maintaining auditory function
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Results from the Vardanyan Lab describe how K+ ions partially dehydrate to pass the narrow construction site in the KCNQ1 channels and why mutations in this site are clinically relevant for patients with long QT syndrome.
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
December 15, 2024 at 11:21 AM
Results from the Vardanyan Lab describe how K+ ions partially dehydrate to pass the narrow construction site in the KCNQ1 channels and why mutations in this site are clinically relevant for patients with long QT syndrome.
www.pnas.org/doi/10.1073/...
www.pnas.org/doi/10.1073/...
December 13, 2024 at 12:24 PM
Cartoon representations of the fast inactivation process in WT Naᵥ channel and CW mutant. Liu and Bezanilla show that a sodium channel mutant removes fast inactivation with the inactivation particle bound.
rupress.org/jgp/article/...
rupress.org/jgp/article/...
A sodium channel mutant removes fast inactivation with the inactivation particle bound | Journal of General Physiology | Rockefeller University Press
In this work, Liu and Bezanilla describe a voltage-gated sodium channel mutant that removes fast inactivation while the fast inactivation particle, the IFM
rupress.org
December 11, 2024 at 9:04 PM
Cartoon representations of the fast inactivation process in WT Naᵥ channel and CW mutant. Liu and Bezanilla show that a sodium channel mutant removes fast inactivation with the inactivation particle bound.
rupress.org/jgp/article/...
rupress.org/jgp/article/...
native cryo-EM structure of the Dystrophin-Glycoprotein Complex (DGC)
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Native DGC structure rationalizes muscular dystrophy-causing mutations - Nature
The detailed structure of the native dystrophin–glycoprotein complex provides mechanistic insights into mutations underlying muscular dystrophy.
www.nature.com
December 11, 2024 at 9:01 PM
native cryo-EM structure of the Dystrophin-Glycoprotein Complex (DGC)
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Ion coupling and inhibitory mechanisms of the human presynaptic high-affinity choline transporter CHT1 | Structure
cell.com/structure/full…
cell.com/structure/full…
https://cell.com/structure/full…
December 11, 2024 at 4:12 PM
Ion coupling and inhibitory mechanisms of the human presynaptic high-affinity choline transporter CHT1 | Structure
cell.com/structure/full…
cell.com/structure/full…
An other impressive structure from Yan Zhao’s group. Here the human choline transporter CHT1
www.cell.com/structure/ab...
www.cell.com/structure/ab...
Ion coupling and inhibitory mechanisms of the human presynaptic high-affinity choline transporter CHT1
Qiu et al. report the cryo-EM structures of the human high-affinity choline transporter 1 (CHT1) with bound inhibitors, the substrate choline, and a substrate-free state. These structures reveal the binding modes of inhibitors with different chemical structures and shed light on the ion-coupled substrate transport mechanism of CHT1.
www.cell.com
December 11, 2024 at 4:10 PM
An other impressive structure from Yan Zhao’s group. Here the human choline transporter CHT1
www.cell.com/structure/ab...
www.cell.com/structure/ab...
By solving the cryo-EM structures of human and mouse FcεRI bound to IgE-Fc, Zhang et al. found species-specific differences in interactions between IgE and the extracellular domains of the receptors.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Architecture of the high-affinity immunoglobulin E receptor
Structural analysis reveals species-specific differences in how the antibody IgE binds to its receptor FcεRI.
www.science.org
December 11, 2024 at 2:28 PM
By solving the cryo-EM structures of human and mouse FcεRI bound to IgE-Fc, Zhang et al. found species-specific differences in interactions between IgE and the extracellular domains of the receptors.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Cryo-EM of GABRP reveals a clear GABA-induced transition controlling channel pore size
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
GABAA receptor π forms channels that stimulate ERK through a G-protein-dependent pathway
GABAA receptor π (GABRP) can form homopentameric channel-like structures, similar to the more common heteromeric type-A GABA receptor channels. However, GABRP activation by GABA uniquely induces a G-protein-signaling pathway rather than allowing chloride passage. Selective inhibition of GABRP signaling may hold therapeutic promise for GABRP-associated cancers.
www.cell.com
December 9, 2024 at 6:08 PM
Cryo-EM of GABRP reveals a clear GABA-induced transition controlling channel pore size
www.cell.com/molecular-ce...
www.cell.com/molecular-ce...
Mindell and Wang Labs use cryo-EM and biochemical assays, to establish the structural basis of the anionic substrate specificity of NaDC3, to elucidate its conformational changes from outward facing to inward facing and to understand the mechanisms of its inhibition. Cool stuff!
rdcu.be/d2Upe
rdcu.be/d2Upe
Substrate translocation and inhibition in human dicarboxylate transporter NaDC3
Nature Structural & Molecular Biology - The authors show cryo-electron microscopy structures of the human high-affinity sodium–dicarboxylate cotransporter, responsible for dicarboxylate...
rdcu.be
December 9, 2024 at 9:43 AM
Mindell and Wang Labs use cryo-EM and biochemical assays, to establish the structural basis of the anionic substrate specificity of NaDC3, to elucidate its conformational changes from outward facing to inward facing and to understand the mechanisms of its inhibition. Cool stuff!
rdcu.be/d2Upe
rdcu.be/d2Upe
Structural insights into the force-transducing mechanism of a motor-stator complex important for bacterial outer membrane lipid homeostasis
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org
December 8, 2024 at 1:36 PM
Structural insights into the force-transducing mechanism of a motor-stator complex important for bacterial outer membrane lipid homeostasis
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
Description of two unrelated individuals who have the same undescribed congenital pleiotropic disorder, and who carry unique monoallelic variants of the LRRC8C gene.
www.embopress.org/doi/full/10....
www.embopress.org/doi/full/10....
De novo variants in LRRC8C resulting in constitutive channel activation cause a human multisystem disorder | The EMBO Journal
imageimageVolume-regulated ion channels (VRACs) open in response to hypotonic stress and protect against excessive cell swelling and bursting. This study describes two patient variants in a VRAC subunit that cause a severe pleiotropic disorder ...
www.embopress.org
December 7, 2024 at 1:55 PM
Description of two unrelated individuals who have the same undescribed congenital pleiotropic disorder, and who carry unique monoallelic variants of the LRRC8C gene.
www.embopress.org/doi/full/10....
www.embopress.org/doi/full/10....
From Bryan Roth’s lab: Structure-guided design of a peripherally restricted chemogenetic system. Out in Cell
www.cell.com/cell/abstrac...
www.cell.com/cell/abstrac...
Structure-guided design of a peripherally restricted chemogenetic system
Kang et al. present HCAD, a peripherally restricted DREADD based on HCA2, with a chemical actuator that avoids crossing the blood-brain barrier and selectively reduces pain in mice. This system will enable the study of peripheral physiology without affecting the central nervous system.
www.cell.com
December 4, 2024 at 9:19 PM
From Bryan Roth’s lab: Structure-guided design of a peripherally restricted chemogenetic system. Out in Cell
www.cell.com/cell/abstrac...
www.cell.com/cell/abstrac...
Mechanism of ceramide synthase inhibition by fumonisin B1.
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Mechanism of ceramide synthase inhibition by fumonisin B1
Ceramide synthases (CerSs) play crucial roles in sphingolipid metabolism and have emerged as promising drug targets for metabolic diseases, cancers, a…
www.sciencedirect.com
December 1, 2024 at 8:47 PM
Mechanism of ceramide synthase inhibition by fumonisin B1.
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
A new transporter revealed.
Magnesium Transporter MgtA revealed as a Dimeric P-type ATPase. Check the #cryoEM #structure of this #membrane #protein in the UniTmp database.
pdbtm.unitmp.org
December 1, 2024 at 8:43 PM
A new transporter revealed.