Karl Romanowicz
@kroman.bsky.social
Principal Scientist @synplexity.bsky.social
Synthetic Biology and Bioinformatics
Synthetic Biology and Bioinformatics
Pinned
Karl Romanowicz
@kroman.bsky.social
· Aug 16
Exploring antibiotic resistance in diverse homologs of the dihydrofolate reductase protein family through broad mutational scanning
DropSynth technology enables scalable and cost-effective exploration of antibiotic resistance across the DHFR protein family.
www.science.org
Our new Science Advances paper is out! 🎉 The Plesa Lab's first, it builds on DropSynth technology as a proof of concept for large-scale synthetic gene libraries, showcasing a synthetic metagenomics approach to studying antibiotic resistance at scale. 👉 www.science.org/doi/10.1126/...
🎥 My Variant Effects Seminar Series (VESS) talk is now on YouTube! I discuss how DropSynth-based gene synthesis reveals how bacterial DHFR variants evolve antibiotic resistance. Thanks to @varianteffect.bsky.social for the invite!
🔗 www.youtube.com/watch?v=KiA9...
#VESS #VariantEffects #DropSynth
🔗 www.youtube.com/watch?v=KiA9...
#VESS #VariantEffects #DropSynth
"Large-scale synthetic gene libraries for decoding variant effects in antibiotic resistance"
YouTube video by Variant Effects
www.youtube.com
October 10, 2025 at 11:26 PM
🎥 My Variant Effects Seminar Series (VESS) talk is now on YouTube! I discuss how DropSynth-based gene synthesis reveals how bacterial DHFR variants evolve antibiotic resistance. Thanks to @varianteffect.bsky.social for the invite!
🔗 www.youtube.com/watch?v=KiA9...
#VESS #VariantEffects #DropSynth
🔗 www.youtube.com/watch?v=KiA9...
#VESS #VariantEffects #DropSynth
Reposted by Karl Romanowicz
Next up! Two applications of MAVE!
🧬 DNA damage repair & cancer: Shelby Hemker (@jacobkitzman.bsky.social lab UMich) measures DNA repair of MUTYH variants.
🦠Antibiotic resistance: @kroman.bsky.social (@synplexity.bsky.social Univ. of Oregon) decodes how mutations drive resistance in DHFR.
🧬 DNA damage repair & cancer: Shelby Hemker (@jacobkitzman.bsky.social lab UMich) measures DNA repair of MUTYH variants.
🦠Antibiotic resistance: @kroman.bsky.social (@synplexity.bsky.social Univ. of Oregon) decodes how mutations drive resistance in DHFR.
September 3, 2025 at 5:40 PM
Next up! Two applications of MAVE!
🧬 DNA damage repair & cancer: Shelby Hemker (@jacobkitzman.bsky.social lab UMich) measures DNA repair of MUTYH variants.
🦠Antibiotic resistance: @kroman.bsky.social (@synplexity.bsky.social Univ. of Oregon) decodes how mutations drive resistance in DHFR.
🧬 DNA damage repair & cancer: Shelby Hemker (@jacobkitzman.bsky.social lab UMich) measures DNA repair of MUTYH variants.
🦠Antibiotic resistance: @kroman.bsky.social (@synplexity.bsky.social Univ. of Oregon) decodes how mutations drive resistance in DHFR.
Our new Science Advances paper is out! 🎉 The Plesa Lab's first, it builds on DropSynth technology as a proof of concept for large-scale synthetic gene libraries, showcasing a synthetic metagenomics approach to studying antibiotic resistance at scale. 👉 www.science.org/doi/10.1126/...
Exploring antibiotic resistance in diverse homologs of the dihydrofolate reductase protein family through broad mutational scanning
DropSynth technology enables scalable and cost-effective exploration of antibiotic resistance across the DHFR protein family.
www.science.org
August 16, 2025 at 6:30 AM
Our new Science Advances paper is out! 🎉 The Plesa Lab's first, it builds on DropSynth technology as a proof of concept for large-scale synthetic gene libraries, showcasing a synthetic metagenomics approach to studying antibiotic resistance at scale. 👉 www.science.org/doi/10.1126/...
Reposted by Karl Romanowicz
We unlock breakthrough insights by delivering the best training sets in biology—at unprecedented scale. youtu.be/KuCaJTPHM2o
Save 90% on DNA variant libraries today
YouTube video by IndieBio
youtu.be
March 26, 2025 at 9:48 PM
We unlock breakthrough insights by delivering the best training sets in biology—at unprecedented scale. youtu.be/KuCaJTPHM2o
Permafrost thaw takes center stage on Ice & Fire Climate Change podcast! 🎙️❄️🔥
S2 Ep2 "Layer" & Ep3 "Microbes Reawaken" explore carbon release & climate impacts. Hear my research!
Tune in: iceandfireclimatepodcast.weebly.com/s2-permafros...
#ClimateChange #PermafrostResearch #SciComm
S2 Ep2 "Layer" & Ep3 "Microbes Reawaken" explore carbon release & climate impacts. Hear my research!
Tune in: iceandfireclimatepodcast.weebly.com/s2-permafros...
#ClimateChange #PermafrostResearch #SciComm
s2: permafrost thaw
podcast is available for listening on Apple Podcasts, Spotify, Overcast, and through the Public Radio Exchange (PRX) or listen below. Press "More Info" in each episode player to reveal show...
iceandfireclimatepodcast.weebly.com
February 19, 2025 at 5:53 PM
Permafrost thaw takes center stage on Ice & Fire Climate Change podcast! 🎙️❄️🔥
S2 Ep2 "Layer" & Ep3 "Microbes Reawaken" explore carbon release & climate impacts. Hear my research!
Tune in: iceandfireclimatepodcast.weebly.com/s2-permafros...
#ClimateChange #PermafrostResearch #SciComm
S2 Ep2 "Layer" & Ep3 "Microbes Reawaken" explore carbon release & climate impacts. Hear my research!
Tune in: iceandfireclimatepodcast.weebly.com/s2-permafros...
#ClimateChange #PermafrostResearch #SciComm
Reposted by Karl Romanowicz
New preprint! We carried out the largest complementation and inhibition study for diverse homologs in a single protein family (DHFR) to date. Highlights thread follows... 1/n 🧵
Exploring Antibiotic Resistance in Diverse Homologs of the Dihydrofolate Reductase Protein Family through Broad Mutational Scanning https://www.biorxiv.org/content/10.1101/2025.01.23.634126v1
January 28, 2025 at 1:27 AM
New preprint! We carried out the largest complementation and inhibition study for diverse homologs in a single protein family (DHFR) to date. Highlights thread follows... 1/n 🧵