Kaitlyn Abe
@kaitlynabe.bsky.social
Biophysics Grad Student @ UW Madison in the Lim Lab || UC Davis Alum || she/her
Reposted by Kaitlyn Abe
Our paper in Science is out! @souravagrawal.bsky.social, @rlynn.bsky.social, @susvirkar.bsky.social, and the rest of the team show human RPA is a telomerase processivity factor essential for telomere maintenance. This reshapes our thinking about telomerase regulation. www.science.org/doi/10.1126/...
Human RPA is an essential telomerase processivity factor for maintaining telomeres
Telomerase counteracts telomere shortening by repeatedly adding DNA repeats to chromosome ends. We identified the replication protein A (RPA) heterotrimer as a telomerase processivity factor critical ...
www.science.org
October 30, 2025 at 10:07 PM
Our paper in Science is out! @souravagrawal.bsky.social, @rlynn.bsky.social, @susvirkar.bsky.social, and the rest of the team show human RPA is a telomerase processivity factor essential for telomere maintenance. This reshapes our thinking about telomerase regulation. www.science.org/doi/10.1126/...
Reposted by Kaitlyn Abe
Our new preprint revealing a new mechanism of mitochondrial disease pathophysiology: the accumulation of toxic complex I intermediates bound to complex III
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org
June 19, 2025 at 6:55 PM
Our new preprint revealing a new mechanism of mitochondrial disease pathophysiology: the accumulation of toxic complex I intermediates bound to complex III
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
I am thrilled to share that our manuscript on using LEA proteins to prevent air-water interface damage to fragile samples for cryo-EM is officially out! #CryoEM
doi.org/10.1038/s414...
doi.org/10.1038/s414...
September 4, 2024 at 6:56 PM
I am thrilled to share that our manuscript on using LEA proteins to prevent air-water interface damage to fragile samples for cryo-EM is officially out! #CryoEM
doi.org/10.1038/s414...
doi.org/10.1038/s414...
Reposted by Kaitlyn Abe
1/3: Led by @kaitlynabe.bsky.social, we found small LEA proteins from nematodes and tardigrades (water bears!) to be incredibly effective in protecting two test fragile complexes - human DNA polymerase alpha-primase and polycomb repressive complex 2 - from AWI damage during plunge freezing.
February 9, 2024 at 2:40 AM
1/3: Led by @kaitlynabe.bsky.social, we found small LEA proteins from nematodes and tardigrades (water bears!) to be incredibly effective in protecting two test fragile complexes - human DNA polymerase alpha-primase and polycomb repressive complex 2 - from AWI damage during plunge freezing.
Reposted by Kaitlyn Abe
Exhausted with trying multiple ways to combat the air-water interface (AWI) problem during sample grid plunge freezing? We may have an easy solution for you!
Read our latest preprint on how we use LEA proteins as AWI sample protectants for challenging samples: www.biorxiv.org/content/10.1...
Read our latest preprint on how we use LEA proteins as AWI sample protectants for challenging samples: www.biorxiv.org/content/10.1...
Small LEA proteins as an effective air-water interface protectant for fragile samples during cryo-EM grid plunge freezing
Sample loss due to air-water interface (AWI) interactions is a significant challenge during cryo-electron microscopy (cryo-EM) sample grid plunge freezing. We report that small Late Embryogenesis Abundant (LEA) proteins, which naturally bind to AWI, can protect samples from AWI damage during plunge freezing. This protection is demonstrated with two LEA proteins from nematodes and tardigrades, which rescued the cryo-EM structural determination outcome of two fragile multisubunit protein complexes. ### Competing Interest Statement The authors have declared no competing interest.
www.biorxiv.org
February 9, 2024 at 2:35 AM
Exhausted with trying multiple ways to combat the air-water interface (AWI) problem during sample grid plunge freezing? We may have an easy solution for you!
Read our latest preprint on how we use LEA proteins as AWI sample protectants for challenging samples: www.biorxiv.org/content/10.1...
Read our latest preprint on how we use LEA proteins as AWI sample protectants for challenging samples: www.biorxiv.org/content/10.1...
I am excited to share that my work with @cijilim.bsky.social on protecting fragile samples from air-water-interface damage during cryo-EM plunge freezing is in preprint! www.biorxiv.org/content/10.1...
February 9, 2024 at 3:12 AM
I am excited to share that my work with @cijilim.bsky.social on protecting fragile samples from air-water-interface damage during cryo-EM plunge freezing is in preprint! www.biorxiv.org/content/10.1...