Honglue
@honglue.bsky.social
NIH/NIGMS K99 fellow in the lab of @doudna-lab.bsky.social at UC Berkeley
Previously JCC fellow in the Doudna lab and a PhD student in the Al-Hashimi lab
Previously JCC fellow in the Doudna lab and a PhD student in the Al-Hashimi lab
The plasmids associated with this paper are available on addgene now! www.addgene.org/browse/artic...
September 18, 2025 at 4:44 AM
The plasmids associated with this paper are available on addgene now! www.addgene.org/browse/artic...
Welcome to the JCC family!
We're thrilled to announce the 2025 class of Jane Coffin Childs Fellows!
See our latest blog post and "look book" to learn more about our newest Fellows and their exciting research!
www.jccfund.org/blog/the-jan...
www.jccfund.org/wp-content/u...
See our latest blog post and "look book" to learn more about our newest Fellows and their exciting research!
www.jccfund.org/blog/the-jan...
www.jccfund.org/wp-content/u...
www.jccfund.org
June 24, 2025 at 4:06 PM
Welcome to the JCC family!
Our paper is finally out in Molecular Cell! 🚀 We uncover why PAM-relaxed Cas9 variants like SpRY are inefficient — they get kinetically trapped during target engagement. Mechanistic insights like this are key to engineering smarter, faster genome editors. Huge thanks to the team! #CRISPR #editing🔬🧬
Online Now: Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing Online now:
Rapid two-step target capture ensures efficient CRISPR-Cas9-guided genome editing
A two-step target capture process—specific but low-affinity PAM binding followed by rapid DNA unwinding—underpins efficient CRISPR-Cas9 editing. Shi et al. show that PAM-relaxed Cas9 variants like SpRY become kinetically trapped between these steps, reducing editing efficiency. These insights provide new principles for engineering improved RNA-guided genome editors.
dlvr.it
May 4, 2025 at 11:46 PM
Our paper is finally out in Molecular Cell! 🚀 We uncover why PAM-relaxed Cas9 variants like SpRY are inefficient — they get kinetically trapped during target engagement. Mechanistic insights like this are key to engineering smarter, faster genome editors. Huge thanks to the team! #CRISPR #editing🔬🧬
Reposted by Honglue
Now online at Molecular Cell! What makes SpyCas9 such an efficient editor? Read more at: www.cell.com/molecular-ce...
Congrats @honglue.bsky.social, Noor Al-Sayyad, and @kevinwasko.bsky.social!
Congrats @honglue.bsky.social, Noor Al-Sayyad, and @kevinwasko.bsky.social!
April 23, 2025 at 9:49 PM
Now online at Molecular Cell! What makes SpyCas9 such an efficient editor? Read more at: www.cell.com/molecular-ce...
Congrats @honglue.bsky.social, Noor Al-Sayyad, and @kevinwasko.bsky.social!
Congrats @honglue.bsky.social, Noor Al-Sayyad, and @kevinwasko.bsky.social!
Reposted by Honglue
“What we’ve done here is uncover the secret sauce that makes typical #Cas9 — the original Nobel molecule — so efficient and precise." – Co-first author @honglue.bsky.social of the @doudna-lab.bsky.social on his new paper out today in Molecular Cell
Read here: shorturl.at/p664D
Read here: shorturl.at/p664D
April 23, 2025 at 7:12 PM
“What we’ve done here is uncover the secret sauce that makes typical #Cas9 — the original Nobel molecule — so efficient and precise." – Co-first author @honglue.bsky.social of the @doudna-lab.bsky.social on his new paper out today in Molecular Cell
Read here: shorturl.at/p664D
Read here: shorturl.at/p664D
See details in this website
gess.hms.harvard.edu/event/meirui...
gess.hms.harvard.edu/event/meirui...
April 3, 2025 at 10:15 PM
See details in this website
gess.hms.harvard.edu/event/meirui...
gess.hms.harvard.edu/event/meirui...
Reposted by Honglue
🧪🧪🧪10 incredible findings about Cas10-relative, mCpol:
result number 10 will surprise you!🧪🧪🧪
@erinedoherty.bsky.social and I teamed up to understand the role and function of Cas10-relative, mCpol, and its role in antiphage immunity. For more, check out Erin's thread 👇
result number 10 will surprise you!🧪🧪🧪
@erinedoherty.bsky.social and I teamed up to understand the role and function of Cas10-relative, mCpol, and its role in antiphage immunity. For more, check out Erin's thread 👇
Preprint alert! ✨ In this project that I co-led with @benadler.bsky.social, we show that a miniature CRISPR-Cas10-like enzyme, mCpol, uses a novel inverse signaling mechanism to prevent the spread of viruses that attempt immune evasion by depleting host cyclic nucleotides.
Check it out:
Check it out:
A miniature CRISPR-Cas10 enzyme confers immunity by an inverse signaling pathway
Microbial and viral co-evolution has created immunity mechanisms involving oligonucleotide signaling that share mechanistic features with human anti-viral systems. In these pathways, including CBASS a...
www.biorxiv.org
March 31, 2025 at 5:31 PM
🧪🧪🧪10 incredible findings about Cas10-relative, mCpol:
result number 10 will surprise you!🧪🧪🧪
@erinedoherty.bsky.social and I teamed up to understand the role and function of Cas10-relative, mCpol, and its role in antiphage immunity. For more, check out Erin's thread 👇
result number 10 will surprise you!🧪🧪🧪
@erinedoherty.bsky.social and I teamed up to understand the role and function of Cas10-relative, mCpol, and its role in antiphage immunity. For more, check out Erin's thread 👇
Reposted by Honglue
Catch @honglue.bsky.social at the Harvard Genome Engineering Seminar Series this Monday, April 7 at 1pm EST / 10am PST! If you’ve ever wondered why SpyCas9 is so efficient at genome editing, don’t miss his talk—Zoom link: harvard.zoom.us/j/94394339529
Giving a seminar for the Genome Engineering Seminar Series at Harvard this Monday, April 7 at 1pm EST / 10am PST! 🎙️ I’ll be talking about our Cas9 work—if you’ve ever wondered what the recipe is for high-efficiency CRISPR genome editing, come hang out!🧬💥
📍 Zoom link: harvard.zoom.us/j/94394339529
📍 Zoom link: harvard.zoom.us/j/94394339529
April 3, 2025 at 2:53 PM
Catch @honglue.bsky.social at the Harvard Genome Engineering Seminar Series this Monday, April 7 at 1pm EST / 10am PST! If you’ve ever wondered why SpyCas9 is so efficient at genome editing, don’t miss his talk—Zoom link: harvard.zoom.us/j/94394339529
Giving a seminar for the Genome Engineering Seminar Series at Harvard this Monday, April 7 at 1pm EST / 10am PST! 🎙️ I’ll be talking about our Cas9 work—if you’ve ever wondered what the recipe is for high-efficiency CRISPR genome editing, come hang out!🧬💥
📍 Zoom link: harvard.zoom.us/j/94394339529
📍 Zoom link: harvard.zoom.us/j/94394339529
April 3, 2025 at 6:57 AM
Giving a seminar for the Genome Engineering Seminar Series at Harvard this Monday, April 7 at 1pm EST / 10am PST! 🎙️ I’ll be talking about our Cas9 work—if you’ve ever wondered what the recipe is for high-efficiency CRISPR genome editing, come hang out!🧬💥
📍 Zoom link: harvard.zoom.us/j/94394339529
📍 Zoom link: harvard.zoom.us/j/94394339529
Reposted by Honglue
Preprint alert! ✨ In this project that I co-led with @benadler.bsky.social, we show that a miniature CRISPR-Cas10-like enzyme, mCpol, uses a novel inverse signaling mechanism to prevent the spread of viruses that attempt immune evasion by depleting host cyclic nucleotides.
Check it out:
Check it out:
A miniature CRISPR-Cas10 enzyme confers immunity by an inverse signaling pathway
Microbial and viral co-evolution has created immunity mechanisms involving oligonucleotide signaling that share mechanistic features with human anti-viral systems. In these pathways, including CBASS a...
www.biorxiv.org
March 31, 2025 at 4:27 PM
Preprint alert! ✨ In this project that I co-led with @benadler.bsky.social, we show that a miniature CRISPR-Cas10-like enzyme, mCpol, uses a novel inverse signaling mechanism to prevent the spread of viruses that attempt immune evasion by depleting host cyclic nucleotides.
Check it out:
Check it out:
Reposted by Honglue
Thanks to out @ucberkeleyofficial.bsky.social community for joining us at Stand Up For Science! @jenniferdoudna.bsky.social spoke about how NIH funding supported her PhD & NSF funding supported her development of #CRISPR genome editing. Federal funding is needed for life-saving science!
March 8, 2025 at 12:03 AM
Thanks to out @ucberkeleyofficial.bsky.social community for joining us at Stand Up For Science! @jenniferdoudna.bsky.social spoke about how NIH funding supported her PhD & NSF funding supported her development of #CRISPR genome editing. Federal funding is needed for life-saving science!