Analytical and Quantitative Light Microscopy Course
aqlm.bsky.social
Analytical and Quantitative Light Microscopy Course
@aqlm.bsky.social
This is the account for the Analytical and Quantitative Light Microscopy Course at the Marine Biological Laboratory in Woods Hole MA. We love all things microscopy!
Applications are now open for AQLM 2026! Join us @mblscience.bsky.social April 29th-May 8th, 2026 for a comprehensive, hands-on light microscopy course with an amazing roster of faculty who are leading experts in their fields!
November 7, 2025 at 2:29 PM
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AQLM was an intense 9-day dive into advanced microscopy—blending core concepts, cutting-edge methods, and inspiring MBL history. I gained valuable skills and connections to better support LSU researchers and strengthen our imaging community. #AQLM2025 #MBL #LightMicroscopy #LSU #SIF #Microscopy 🔬🦑
May 13, 2025 at 2:13 AM
And just like that, #AQLM2025 is over. Hopefully we’ll all find ourselves in the same windowless microscope room sometime soon, bead slide in hand.
May 9, 2025 at 1:42 PM
This year’s esteemed Inoue Lecture was given by French optical physicist Dr. Sophie Brasselet of The Fresnel Institute. Dr. Brasselet spoke of her team’s efforts towards applying 6 degrees of PSF engineering to super resolution polarization microscopy. #AQLM2025
May 9, 2025 at 1:43 AM
As a final exercise everyone got to build open source microscopes using kits available from UC2 @openuc2.bsky.social #AQLM2025
May 9, 2025 at 1:28 AM
The machines learned this morning. Dr. Daniel Cortes led a lab on machine learning for image processing.
May 8, 2025 at 7:33 PM
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May 8, 2025 at 6:35 PM
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Beautiful plant! Captured with our #lightsheet microsocpe at the #AQLM2025 @mblscience.bsky.social! Curious? openuc2.com/light-sheet-3/
May 7, 2025 at 8:25 PM
Our last imaging lab was this morning (!) On to analysis and then we build more scopes and celebrate the end 🥲
May 7, 2025 at 8:39 PM
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🔬 Glad to support #AQLM2025 at the #MBL.

On our partners’ workstations:
✅ UNO+ – user-friendly & high value
✅ BL3 – with Full Sample Feedback for real-time control of Temp, Humidity & CO₂ at the sample level.

👉 Already using our latest tech? Tell us what u think!
May 7, 2025 at 12:26 PM
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May 7, 2025 at 5:31 PM
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The AQLM Queens of Super-res lead evening discussion. Dr. Gustavsson and Dr. Lidke help us figure out how to look REALLY close. #AQLM2025

@gustavssonlab.bsky.social
@aqlm.bsky.social
May 7, 2025 at 2:49 AM
Arabidopsis labeled with EB1b-mNeon in green (microtubule plus end) and tubulin in magenta (microtubules). Timelapse image on the Leica Stellaris 8 laser scanning confocal. @leicamicrosystems.bsky.social @mblscience.bsky.social #AQLM2025
May 6, 2025 at 11:10 PM
Ooof, those PSFs are looking *a little* asymmetric...sample prep is key here! (AKA keep Cheeto dust out of your bead sample.)
#AQLM2025
Just a bunch of demonic point-spread functions 👹😈👺🔬

#aqlm2025 #microscopy #confocal #microscope #fluorescence
May 6, 2025 at 4:27 PM
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May 6, 2025 at 1:44 PM
Dr. Palmer gives an amazing lecture on dyes, fluorescent proteins, and FRET/FLIM. A chemist who makes non-chemists understand the chemistry! #AQLM2025
Lots of fun and great conversation about fluorescent proteins, probes, biosensors and Fret at @aqlm.bsky.social course at MBL. Also great to be in such close proximity to Shimomura GFP!
May 6, 2025 at 1:16 AM
Arabidopsis with EB1b-mNEON labeling the plus end of microtubules 🌱 Imaged on the Nikon AX-R laser scanning confocal with NSPARC. #AQLM2025
May 5, 2025 at 8:47 PM
Live cell lab: understanding the trade offs between acquisition time, SNR, spatial resolution, and cell survival on different microscope systems. You can’t have it all, but ideally you can have enough for meaningful analysis. #AQLM2025
May 5, 2025 at 7:13 PM
The back focal plane! Today Dr. Chris Bjornsson demonstrated Abbe’s theory of resolution by manipulating the light passing through the back focal plane. Here he is using a lens to focus the back focal plane onto the camera for us to see. #AQLM2025
May 4, 2025 at 9:59 PM
Dr. Shalin Mehta giving a tour of Shinya Inoue’s polarization scope housed in Lillie @mblscience.bsky.social 🔬
#AQLM2025
May 4, 2025 at 6:53 PM
FRET tonight! This is the Leica Stellaris 8, part of the MBL Imaging Innovation Center. #AQLM2025
May 4, 2025 at 3:03 AM
Watch how the color shifts as the angle changes.
#AQLM2025
May 4, 2025 at 2:59 AM
Sugar under polarized light 🤩
#AQLM2025
May 3, 2025 at 11:38 PM
Rabbit muscle fibers under darkfield. Something interesting is happening where two fibers overlap…
#AQLM2025
May 3, 2025 at 11:35 PM
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May 3, 2025 at 7:04 PM