Srinivas Ramachandran
@4everbiochemist.bsky.social
Reposted by Srinivas Ramachandran
Congratulations to Molecular Biology PhD student Giovana Maria Breda Veronezi of Srinivas Ramachandran's lab @4everbiochemist.bsky.social for successfully completing her PhD defense!
November 7, 2025 at 11:27 PM
Congratulations to Molecular Biology PhD student Giovana Maria Breda Veronezi of Srinivas Ramachandran's lab @4everbiochemist.bsky.social for successfully completing her PhD defense!
Reposted by Srinivas Ramachandran
Some (+)ve news to lighten another heavy weekend: our latest preprint (c/o Mattiroli + Ramani labs) is up!
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)
The eukaryotic replisome intrinsically generates asymmetric daughter chromatin fibers
DNA replication is molecularly asymmetric, due to distinct mechanisms for lagging and leading strand DNA synthesis. Whether chromatin assembly on newly replicated strands is also asymmetric remains un...
www.biorxiv.org
September 20, 2025 at 4:10 PM
Some (+)ve news to lighten another heavy weekend: our latest preprint (c/o Mattiroli + Ramani labs) is up!
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)
www.biorxiv.org/content/10.1...
A tour-de-force by 1st authors Bruna Eckhardt & @palindromephd.bsky.social, focusing on chromatin replication. RTs welcome; tweetorial in 3,2...(1/n)
Reposted by Srinivas Ramachandran
On our three year lab anniversary (give or take a few days!), I'm proud to share the first original work produced within the Meers Lab: Plate-CUT&Tag! Take a look: www.biorxiv.org/content/10.1... 1/3
A protocol for high-throughput microplate-based CUT&Tag
Cleavage Under Targets & Tagmentation (CUT&Tag) is a versatile method for measuring genomic occupancy of chromatin-associated proteins with high sensitivity and specificity. CUT&Tag has low sequencing...
www.biorxiv.org
September 22, 2025 at 4:38 PM
On our three year lab anniversary (give or take a few days!), I'm proud to share the first original work produced within the Meers Lab: Plate-CUT&Tag! Take a look: www.biorxiv.org/content/10.1... 1/3
Reposted by Srinivas Ramachandran
In yeast spores, polarity emerges from a different playbook.
Dr. Michael McMurray (University of Colorado Anschutz) reveals how Bud8, Bud5, and Cdc42 machinery help spores establish polarity without relying on classic cortical landmarks.
www.molbiolcell.org/doi/10.1091/... #ASCB #CellBiology
Dr. Michael McMurray (University of Colorado Anschutz) reveals how Bud8, Bud5, and Cdc42 machinery help spores establish polarity without relying on classic cortical landmarks.
www.molbiolcell.org/doi/10.1091/... #ASCB #CellBiology
August 22, 2025 at 12:05 PM
In yeast spores, polarity emerges from a different playbook.
Dr. Michael McMurray (University of Colorado Anschutz) reveals how Bud8, Bud5, and Cdc42 machinery help spores establish polarity without relying on classic cortical landmarks.
www.molbiolcell.org/doi/10.1091/... #ASCB #CellBiology
Dr. Michael McMurray (University of Colorado Anschutz) reveals how Bud8, Bud5, and Cdc42 machinery help spores establish polarity without relying on classic cortical landmarks.
www.molbiolcell.org/doi/10.1091/... #ASCB #CellBiology
Reposted by Srinivas Ramachandran
Our paper is out in its final form! Back in April 2023, my coauthor Aleks Radakovic, then a PhD student, approached me after a talk in Chicago to ask what I thought an aminoacylated tRNA would look like if we pulled it through a @nanopore.
doi.org/10.1038/s414...
doi.org/10.1038/s414...
Nanopore sequencing of intact aminoacylated tRNAs - Nature Communications
Accurate protein synthesis depends on aminoacylated tRNAs, but their identities have been hard to measure. Here, authors present aa-tRNA-seq, a nanopore-based method that reveals the amino acid, seque...
doi.org
August 22, 2025 at 9:08 PM
Our paper is out in its final form! Back in April 2023, my coauthor Aleks Radakovic, then a PhD student, approached me after a talk in Chicago to ask what I thought an aminoacylated tRNA would look like if we pulled it through a @nanopore.
doi.org/10.1038/s414...
doi.org/10.1038/s414...
Reposted by Srinivas Ramachandran
Why do #StemCells have lower levels of facultative #heterochromatin, defined by H3K27me3, compared to differentiated cells? By artificially lengthening G1 phase, @4everbiochemist.bsky.social &co show that G1 length is an essential determinant of H3K27me3 landscape @plosbiology.org 🧪 plos.io/4lKfztO
April 24, 2025 at 8:03 AM
Why do #StemCells have lower levels of facultative #heterochromatin, defined by H3K27me3, compared to differentiated cells? By artificially lengthening G1 phase, @4everbiochemist.bsky.social &co show that G1 length is an essential determinant of H3K27me3 landscape @plosbiology.org 🧪 plos.io/4lKfztO
Reposted by Srinivas Ramachandran
In #G3journal, @4everbiochemist.bsky.social and colleagues assessed the role of essential histone chaperone Spn1 in maintaining #yeast chromatin structure. They found that Spn1 preserves nucleosomal, subnucleosomal, and non-nucleosomal protections across the genome.
Read more: buff.ly/kRe9RH3
Read more: buff.ly/kRe9RH3
April 19, 2025 at 5:02 PM
In #G3journal, @4everbiochemist.bsky.social and colleagues assessed the role of essential histone chaperone Spn1 in maintaining #yeast chromatin structure. They found that Spn1 preserves nucleosomal, subnucleosomal, and non-nucleosomal protections across the genome.
Read more: buff.ly/kRe9RH3
Read more: buff.ly/kRe9RH3
Reposted by Srinivas Ramachandran
Really excited to have contributed (in minor part) to this study from the
@SarthyLab
on chromatin vulnerabilities in CBFA2T3-GLIS2 AMLs. A clever synthesis of chromatin profiling, single-cell RNA, and DepMap data to uncover both mechanism and target 1/6 www.biorxiv.org/content/10.1...
@SarthyLab
on chromatin vulnerabilities in CBFA2T3-GLIS2 AMLs. A clever synthesis of chromatin profiling, single-cell RNA, and DepMap data to uncover both mechanism and target 1/6 www.biorxiv.org/content/10.1...
Chromatin Profiling of CBFA2T3-GLIS2 AMLs Identifies Key Transcription Factor Dependencies and BRG1 ...
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
www.biorxiv.org
September 6, 2023 at 6:11 PM
Really excited to have contributed (in minor part) to this study from the
@SarthyLab
on chromatin vulnerabilities in CBFA2T3-GLIS2 AMLs. A clever synthesis of chromatin profiling, single-cell RNA, and DepMap data to uncover both mechanism and target 1/6 www.biorxiv.org/content/10.1...
@SarthyLab
on chromatin vulnerabilities in CBFA2T3-GLIS2 AMLs. A clever synthesis of chromatin profiling, single-cell RNA, and DepMap data to uncover both mechanism and target 1/6 www.biorxiv.org/content/10.1...
Please read our preprint: "G1 length dictates heterochromatin landscape"
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
www.biorxiv.org
December 7, 2023 at 6:27 PM
Please read our preprint: "G1 length dictates heterochromatin landscape"
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...