With Sven zur Oven-Krockhaus, @luiselotterausch.bsky.social & Klaus Harter we wrote an #ExpertView on #sptPALM in #plants 🌱 (focus: #plasmamembrane).
✔️ Our goal: help you get started with this powerful method 🚀
❓ Curious? We’ll be happy to get you ready!
✔️ Our goal: help you get started with this powerful method 🚀
❓ Curious? We’ll be happy to get you ready!
Check out our latest review on investigating plant plasma membrane proteins with super resolution microscopy 🔬 🌱 in @jxbotany.bsky.social
#superresolution #plasmamembrane
academic.oup.com/jxb/advance-...
#superresolution #plasmamembrane
academic.oup.com/jxb/advance-...
Revealing Plasma Membrane Protein Dynamics in Living Plant Cells with Single-Molecule Tracking
Abstract. The behavior of proteins and other biomolecules in cellular environments is governed by complex molecular processes. Understanding their temporal
academic.oup.com
October 2, 2025 at 8:35 AM
With Sven zur Oven-Krockhaus, @luiselotterausch.bsky.social & Klaus Harter we wrote an #ExpertView on #sptPALM in #plants 🌱 (focus: #plasmamembrane).
✔️ Our goal: help you get started with this powerful method 🚀
❓ Curious? We’ll be happy to get you ready!
✔️ Our goal: help you get started with this powerful method 🚀
❓ Curious? We’ll be happy to get you ready!
Opus Magnum by @loreoliv.bsky.social on the role of DnaA in bacterial DNA replication initiation building on fancy combinations of #sptPALM, proteomics, flow cytometry, genetics, and other techniques. Great collab with the Staals lab and many others. Long 🧵👇
🧵 Our study on the elusive titration control mechanism of the replication initiator DnaA in Escherichia coli is now out on @natcomms.nature.com! @stephkoe.bsky.social
@hohlbeinlab.bsky.social @nicoc-micsynmet.bsky.social @ettema.bsky.social @mib-wur.bsky.social
doi.org/10.1038/s414...
@hohlbeinlab.bsky.social @nicoc-micsynmet.bsky.social @ettema.bsky.social @mib-wur.bsky.social
doi.org/10.1038/s414...
The Escherichia coli replication initiator DnaA is titrated on the chromosome - Nature Communications
A DNA-binding protein, DnaA, regulates the initiation of chromosomal replication in bacteria. Here, Olivi et al. show that the bacterial chromosome sequesters DnaA in a growth rate-dependent manner, s...
doi.org
August 22, 2025 at 9:03 AM
Opus Magnum by @loreoliv.bsky.social on the role of DnaA in bacterial DNA replication initiation building on fancy combinations of #sptPALM, proteomics, flow cytometry, genetics, and other techniques. Great collab with the Staals lab and many others. Long 🧵👇
Excited to share our #bioRxiv #preprint on HIR2, RLKs and membrane organisation in #Arabidopsis. We used many exciting techniques like #sptPALM, #AlphaFold and #BN-PAGE 🌱
Arabidopsis HYPERSENSITIVE INDUCED REACTION 2 affects plasma membrane receptor pathways and organization https://www.biorxiv.org/content/10.1101/2025.04.11.648320v1
April 15, 2025 at 5:24 AM
Excited to share our #bioRxiv #preprint on HIR2, RLKs and membrane organisation in #Arabidopsis. We used many exciting techniques like #sptPALM, #AlphaFold and #BN-PAGE 🌱
ALFA tag even allowed us to explore trifunctional fluorescence complementation (TriFC), targeted protein degradation and super-resolution sptPALM imaging for the notoriously difficult to work with Arabidopsis IRT1 root iron transporter.
March 20, 2025 at 2:05 PM
ALFA tag even allowed us to explore trifunctional fluorescence complementation (TriFC), targeted protein degradation and super-resolution sptPALM imaging for the notoriously difficult to work with Arabidopsis IRT1 root iron transporter.
Presenting our research to the Bsky Community – Part II! 🌱🔬
Check out our #bioRxiv #preprint on #Dual-Color #sptPALM in #Arabidopsis 🌱! Using #PA-GFP & #PATagRFP, we enable simultaneous tracking of two proteins in living plant cells! 🎨🎉
👉 Read more: tinyurl.com/2vcry74m
Check out our #bioRxiv #preprint on #Dual-Color #sptPALM in #Arabidopsis 🌱! Using #PA-GFP & #PATagRFP, we enable simultaneous tracking of two proteins in living plant cells! 🎨🎉
👉 Read more: tinyurl.com/2vcry74m
Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM
Super-resolution microscopy techniques have revolutionized cell biology by providing insights into the dynamics of single molecules and nanoscale organization within living cells. However, the applica...
tinyurl.com
February 8, 2025 at 1:37 PM
Presenting our research to the Bsky Community – Part II! 🌱🔬
Check out our #bioRxiv #preprint on #Dual-Color #sptPALM in #Arabidopsis 🌱! Using #PA-GFP & #PATagRFP, we enable simultaneous tracking of two proteins in living plant cells! 🎨🎉
👉 Read more: tinyurl.com/2vcry74m
Check out our #bioRxiv #preprint on #Dual-Color #sptPALM in #Arabidopsis 🌱! Using #PA-GFP & #PATagRFP, we enable simultaneous tracking of two proteins in living plant cells! 🎨🎉
👉 Read more: tinyurl.com/2vcry74m
#Python, #Seaborn & #Pandas is growing on me. Some synthetic data to plot average diffusion coefficients etc of tracks in individual bacterial cells (independent repeats of different conditions). #sptPALM is hard. Getting there @jmakela.bsky.social + @loreoliv.bsky.social
December 7, 2024 at 8:21 PM
#Python, #Seaborn & #Pandas is growing on me. Some synthetic data to plot average diffusion coefficients etc of tracks in individual bacterial cells (independent repeats of different conditions). #sptPALM is hard. Getting there @jmakela.bsky.social + @loreoliv.bsky.social
The best resource for #microscopy is among us on BlueSky! Savior of my #sptPALM designs
Hello friends! We’re here to celebrate and share all things fluorescent. Tag us as you share findings related to the use or publication of FPs and other probes
November 18, 2024 at 9:01 PM
The best resource for #microscopy is among us on BlueSky! Savior of my #sptPALM designs
This week will hopefully bring the answer to the very important question whether mMaple3 is more photostable than PAmCherry2 in E. coli #sptPALM. Can't wait. @loreoliv.bsky.social
November 18, 2024 at 2:48 PM
This week will hopefully bring the answer to the very important question whether mMaple3 is more photostable than PAmCherry2 in E. coli #sptPALM. Can't wait. @loreoliv.bsky.social
October 23, 2024 at 9:09 AM
Some colleagues bought some #pioreactors for our next set of #sptPALM experiments. Stable for 100+ hours and you can exchange as much buffer as you like after reaching threshold (saw tooth). Excellent value for money it looks like.
October 21, 2024 at 8:07 AM
Some colleagues bought some #pioreactors for our next set of #sptPALM experiments. Stable for 100+ hours and you can exchange as much buffer as you like after reaching threshold (saw tooth). Excellent value for money it looks like.
New preprint spear headed by the amazing #LorenzoOlivi: "The Escherichia coli replication initiator DnaA is titrated on the chromosome" using, among many other techniques, #sptPALM to determine growth dependent mobilities of DnaA diffusing in live cells. doi.org/10.1101/2024.10.07.617004 1/n
The Escherichia coli replication initiator DnaA is titrated on the chromosome
DNA replication initiation is orchestrated in many prokaryotes by the replication initiator DnaA. Two models for regulation of DnaA activity in Escherichia coli have been proposed: the switch between ...
doi.org
October 12, 2024 at 1:37 PM
New preprint spear headed by the amazing #LorenzoOlivi: "The Escherichia coli replication initiator DnaA is titrated on the chromosome" using, among many other techniques, #sptPALM to determine growth dependent mobilities of DnaA diffusing in live cells. doi.org/10.1101/2024.10.07.617004 1/n
📜 Simultaneous and Dynamic Super‐Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual‐color sptPALM
🧑🔬 Leander Rohr, Sven zur Oven‐Krockhaus, et al.
📔 @biorxiv-plants.bsky.social
🔗 www.biorxiv.org/content/10.1...
#️⃣ #PlantScience #PlantMicroscopy #Microscopy #SuperResMicroscopy
🧑🔬 Leander Rohr, Sven zur Oven‐Krockhaus, et al.
📔 @biorxiv-plants.bsky.social
🔗 www.biorxiv.org/content/10.1...
#️⃣ #PlantScience #PlantMicroscopy #Microscopy #SuperResMicroscopy
Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
www.biorxiv.org
March 18, 2024 at 8:10 AM
📜 Simultaneous and Dynamic Super‐Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual‐color sptPALM
🧑🔬 Leander Rohr, Sven zur Oven‐Krockhaus, et al.
📔 @biorxiv-plants.bsky.social
🔗 www.biorxiv.org/content/10.1...
#️⃣ #PlantScience #PlantMicroscopy #Microscopy #SuperResMicroscopy
🧑🔬 Leander Rohr, Sven zur Oven‐Krockhaus, et al.
📔 @biorxiv-plants.bsky.social
🔗 www.biorxiv.org/content/10.1...
#️⃣ #PlantScience #PlantMicroscopy #Microscopy #SuperResMicroscopy
Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM https://www.biorxiv.org/content/10.1101/2024.03.13.584811v1
Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM https://www.biorxiv.org/content/10.1101/2024.03.13.584811v1
Super-resolution microscopy techniques have revolutionized cell biology by providing insights into t
www.biorxiv.org
March 16, 2024 at 1:02 AM
Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM https://www.biorxiv.org/content/10.1101/2024.03.13.584811v1
Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM https://www.biorxiv.org/content/10.1101/2024.03.13.584811v1
Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM https://www.biorxiv.org/content/10.1101/2024.03.13.584811v1
Super-resolution microscopy techniques have revolutionized cell biology by providing insights into t
www.biorxiv.org
March 16, 2024 at 1:02 AM
Simultaneous and Dynamic Super-Resolution Imaging of Two Proteins in Arabidopsis thaliana using dual-color sptPALM https://www.biorxiv.org/content/10.1101/2024.03.13.584811v1
PhD defense with an upgraded setup! Listening to @piponunesv from @GiannoneLab presenting his nice work looking at single adhesion protein by sptPALM during stretch. Feat @ManuelTHERY @PlanarPolarityT @S_LevequeFort C. Grashoff and P. Nassoy
January 2, 2025 at 2:56 PM
PhD defense with an upgraded setup! Listening to @piponunesv from @GiannoneLab presenting his nice work looking at single adhesion protein by sptPALM during stretch. Feat @ManuelTHERY @PlanarPolarityT @S_LevequeFort C. Grashoff and P. Nassoy
Also from prev link: inferenceMAP generates diffusion landscapes from sptPALM data (pdf link http://arxiv.org/pdf/1504.00525.pdf)
December 26, 2024 at 6:16 PM
Also from prev link: inferenceMAP generates diffusion landscapes from sptPALM data (pdf link http://arxiv.org/pdf/1504.00525.pdf)
XD switches to sptPALM of transcription factors: tracking and using angle correlation between trajectory steps to detect constrains #iphria
December 26, 2024 at 4:52 PM
XD switches to sptPALM of transcription factors: tracking and using angle correlation between trajectory steps to detect constrains #iphria