New lab preprint! ERCC6L2 disease is a recessive bone marrow failure syndrome caused by mutations in the putative DNA helicase ERCC6L2. Using mouse genetics, biochemistry and AF3 we uncover ERCC6L2-MRI as a KU-regulatory complex stimulating NHEJ at staggered DSBs: www.biorxiv.org/content/10.1...
The ERCC6L2-MRI-KU complex coordinates NHEJ at staggered DNA double-strand breaks
ERCC6L2 disease is a recessive bone marrow failure (BMF) syndrome caused by mutations in the SNF2-like putative DNA helicase ERCC6L2. While implicated in DNA replication, double strand break (DSB) rep...
www.biorxiv.org
December 1, 2025 at 9:28 PM
New lab preprint! ERCC6L2 disease is a recessive bone marrow failure syndrome caused by mutations in the putative DNA helicase ERCC6L2. Using mouse genetics, biochemistry and AF3 we uncover ERCC6L2-MRI as a KU-regulatory complex stimulating NHEJ at staggered DSBs: www.biorxiv.org/content/10.1...
Livgardens kaserne fra hjørnet af Gothersgade vs DSBs fhv fra hjørnet af Sølvgade
December 2, 2025 at 8:21 AM
Livgardens kaserne fra hjørnet af Gothersgade vs DSBs fhv fra hjørnet af Sølvgade
7/12
Sister-pore-C showed that DSBs locally increase looping and create strong trans-sister contact interactions, putting the DNA broken ends in contact with the sister chromatid.
Sister-pore-C showed that DSBs locally increase looping and create strong trans-sister contact interactions, putting the DNA broken ends in contact with the sister chromatid.
December 4, 2025 at 8:18 PM
7/12
Sister-pore-C showed that DSBs locally increase looping and create strong trans-sister contact interactions, putting the DNA broken ends in contact with the sister chromatid.
Sister-pore-C showed that DSBs locally increase looping and create strong trans-sister contact interactions, putting the DNA broken ends in contact with the sister chromatid.
3/12
To study this, we induced DSBs at defined genomic sites in S/G2-synchronized human cells.
Most breaks were repaired by homologous recombination, allowing us to map the search process precisely.
To study this, we induced DSBs at defined genomic sites in S/G2-synchronized human cells.
Most breaks were repaired by homologous recombination, allowing us to map the search process precisely.
December 4, 2025 at 8:18 PM
3/12
To study this, we induced DSBs at defined genomic sites in S/G2-synchronized human cells.
Most breaks were repaired by homologous recombination, allowing us to map the search process precisely.
To study this, we induced DSBs at defined genomic sites in S/G2-synchronized human cells.
Most breaks were repaired by homologous recombination, allowing us to map the search process precisely.
2/12
DSBs threaten genome stability. Homologous recombination relies on the sister chromatid for faithful repair, but sisters may be distant and misaligned. Classical models invoke genome-wide scanning, but the genome is vast and structured, so how is homology actually found?
DSBs threaten genome stability. Homologous recombination relies on the sister chromatid for faithful repair, but sisters may be distant and misaligned. Classical models invoke genome-wide scanning, but the genome is vast and structured, so how is homology actually found?
December 4, 2025 at 8:18 PM
2/12
DSBs threaten genome stability. Homologous recombination relies on the sister chromatid for faithful repair, but sisters may be distant and misaligned. Classical models invoke genome-wide scanning, but the genome is vast and structured, so how is homology actually found?
DSBs threaten genome stability. Homologous recombination relies on the sister chromatid for faithful repair, but sisters may be distant and misaligned. Classical models invoke genome-wide scanning, but the genome is vast and structured, so how is homology actually found?
Excited to share our new Trends in Genetics review article on how transcriptional addiction drives genome instability in cancer.
We discuss how hypertranscription, super-enhancers, R-loops, and TOP1/TOP2 create promoter- and gene-body DSBs — a true Achilles’ heel of oncogenes.
We discuss how hypertranscription, super-enhancers, R-loops, and TOP1/TOP2 create promoter- and gene-body DSBs — a true Achilles’ heel of oncogenes.
Breaking the script: transcriptional addiction as a driver of genome instability in cancer
Transcription is not only an essential cellular process but also a major source of endogenous DNA strand breaks. Many cancers exhibit transcriptional …
www.sciencedirect.com
November 20, 2025 at 6:06 AM
Excited to share our new Trends in Genetics review article on how transcriptional addiction drives genome instability in cancer.
We discuss how hypertranscription, super-enhancers, R-loops, and TOP1/TOP2 create promoter- and gene-body DSBs — a true Achilles’ heel of oncogenes.
We discuss how hypertranscription, super-enhancers, R-loops, and TOP1/TOP2 create promoter- and gene-body DSBs — a true Achilles’ heel of oncogenes.
Jeg har en times overgang på Køben H, og har for første gang oppdaget og nyttiggjort meg DSBs lounge for første klasse togpassasjerer.
Interrail pass første klasse billett er tilstrekkelig.
I sydenden av stasjonsområdet. Tilgang med heis for eldre damer (og andre).
Kaffeautomat og frukt, gratis.
Interrail pass første klasse billett er tilstrekkelig.
I sydenden av stasjonsområdet. Tilgang med heis for eldre damer (og andre).
Kaffeautomat og frukt, gratis.
November 11, 2025 at 12:01 PM
Jeg har en times overgang på Køben H, og har for første gang oppdaget og nyttiggjort meg DSBs lounge for første klasse togpassasjerer.
Interrail pass første klasse billett er tilstrekkelig.
I sydenden av stasjonsområdet. Tilgang med heis for eldre damer (og andre).
Kaffeautomat og frukt, gratis.
Interrail pass første klasse billett er tilstrekkelig.
I sydenden av stasjonsområdet. Tilgang med heis for eldre damer (og andre).
Kaffeautomat og frukt, gratis.
Jeg er om bord på DSBs nye Talgo-tog mellom København H og Hamburg Hbf.
Første klasse, vogn #23.
Hadde reservasjon sete #13, var noe trangbodd der, så jeg annekterte en ledig firerseksjon for meg selv.
Sete #41. Var fistende å ta nabosetet, #42, men kom til at jeg fikk droppe
1/
Første klasse, vogn #23.
Hadde reservasjon sete #13, var noe trangbodd der, så jeg annekterte en ledig firerseksjon for meg selv.
Sete #41. Var fistende å ta nabosetet, #42, men kom til at jeg fikk droppe
1/
November 11, 2025 at 2:18 PM
Jeg er om bord på DSBs nye Talgo-tog mellom København H og Hamburg Hbf.
Første klasse, vogn #23.
Hadde reservasjon sete #13, var noe trangbodd der, så jeg annekterte en ledig firerseksjon for meg selv.
Sete #41. Var fistende å ta nabosetet, #42, men kom til at jeg fikk droppe
1/
Første klasse, vogn #23.
Hadde reservasjon sete #13, var noe trangbodd der, så jeg annekterte en ledig firerseksjon for meg selv.
Sete #41. Var fistende å ta nabosetet, #42, men kom til at jeg fikk droppe
1/
De første af DSBs nye, spanskbyggede Talgo-vognsæt er kommet i trafik mellem København og Hamburg. I dag markeredes den første afgang mod Hamburg på Københavns Hovedbanegård (selvom det faktisk kun var tredje afgang med passagerer i DSB-regi!). Her ses toget ved København Syd.
Første Talgo-tog mod Hamburg ved København Syd station
En pressemeddelelse fra DSB proklamerede i begyndelsen af november 2025, at der mandag 3. november for første gang ville afgå et passagerførende tog fra Københavns Hovedbanegård mod Hamburg bestående ...
myldretid.dk
November 3, 2025 at 8:38 PM
De første af DSBs nye, spanskbyggede Talgo-vognsæt er kommet i trafik mellem København og Hamburg. I dag markeredes den første afgang mod Hamburg på Københavns Hovedbanegård (selvom det faktisk kun var tredje afgang med passagerer i DSB-regi!). Her ses toget ved København Syd.
RNA:DNA hybrids increase at DSBs, crucial for repair, but form without RNA polymerase recruitment. Discover insights on hybrid accumulation. PMID:40447771, Nat Cell Biol 2025, @NatureCellBio https://doi.org/10.1038/s41556-025-01669-y #Medsky #Pharmsky #RNA #ASHG #ESHG 🧪
Transcriptional repression facilitates RNA:DNA hybrid accumulation at DNA double-strand breaks | Nature Cell Biology
RNA:DNA hybrids accumulate at DNA double-strand breaks (DSBs) and were shown to regulate homologous recombination repair. The mechanism responsible for the formation of these non-canonical RNA:DNA structures remains unclear although they were proposed to arise consequently to RNA polymerase II or III loading followed by DSB-induced de novo transcription at the break site. Here, we found no evidence of RNA polymerase recruitment at DSBs. Rather, strand-specific R-loop mapping revealed that RNA:DNA hybrids are mainly generated at DSBs occurring in transcribing loci, from the hybridization of pre-existing RNA to the 3′ overhang left by DNA end resection. We further identified the H3K4me3 reader spindlin 1 and the transcriptional regulator PAF1 as factors promoting RNA:DNA hybrid accumulation at DSBs, through their role in mediating transcriptional repression in cis to DSBs. Altogether, we provide evidence that RNA:DNA hybrids accumulate at DSBs occurring in transcribing loci as a result o
doi.org
October 30, 2025 at 6:20 AM
Was ist mit Art. 13 (1) e) bei externen DSBs? Könnte sich daraus eine Pflicht ergeben?
October 5, 2023 at 10:01 AM
Was ist mit Art. 13 (1) e) bei externen DSBs? Könnte sich daraus eine Pflicht ergeben?
Og der var Welhaven i gang, men tar ikke høyde for at tilrettelagt og ansvarlig pyrobruk medfører lav risiko. Samtidig som det har blitt dokumentert at DSBs tolkning omskriver lovteksten.
Kommentar: Ulevelig og ulovlig
Et barn ble truffet av bluss i Bergen, mens pyro ble tatt med ut på banen i Bærum. Nå må ansvarlige voksne være bestemte på at norsk lov også gjelder på fotballtribuner.
www.vg.no
November 28, 2023 at 11:55 AM
Og der var Welhaven i gang, men tar ikke høyde for at tilrettelagt og ansvarlig pyrobruk medfører lav risiko. Samtidig som det har blitt dokumentert at DSBs tolkning omskriver lovteksten.
Nine is the ennead of DSBs
July 31, 2025 at 12:18 AM
Nine is the ennead of DSBs
Wann gibt’s diese Therapie auch für DSBs?
January 13, 2025 at 7:35 AM
Wann gibt’s diese Therapie auch für DSBs?
Da er jeg på turens siste togreise - i DSBs behagelige dobbeltdekker mellom København og Helsingør.
Det er sporarbeidsfestival i Sverige i helgen. Buss for tog på to ulike strekninger - med knapp byttetid i Göteborg. Derfor har jeg bestilt buss fra Helsingborg til Oslo - og sparer to timer.
Det er sporarbeidsfestival i Sverige i helgen. Buss for tog på to ulike strekninger - med knapp byttetid i Göteborg. Derfor har jeg bestilt buss fra Helsingborg til Oslo - og sparer to timer.
May 12, 2024 at 10:44 AM
Da er jeg på turens siste togreise - i DSBs behagelige dobbeltdekker mellom København og Helsingør.
Det er sporarbeidsfestival i Sverige i helgen. Buss for tog på to ulike strekninger - med knapp byttetid i Göteborg. Derfor har jeg bestilt buss fra Helsingborg til Oslo - og sparer to timer.
Det er sporarbeidsfestival i Sverige i helgen. Buss for tog på to ulike strekninger - med knapp byttetid i Göteborg. Derfor har jeg bestilt buss fra Helsingborg til Oslo - og sparer to timer.
Un’era presto finita: l’ultimo Kiosk DSB della Danimarca si chiude [DSBs deroute]
https://www.csseo.org/794356/
Un’era presto finita: l’ultimo Kiosk DSB della Danimarca si chiude [DSBs deroute] https://avisendanmark.dk/danmark/en-epoke-snart-slut-danmarks-sidste-dsb-kiosk-lukker di Rubdown2837
https://www.csseo.org/794356/
Un’era presto finita: l’ultimo Kiosk DSB della Danimarca si chiude [DSBs deroute] https://avisendanmark.dk/danmark/en-epoke-snart-slut-danmarks-sidste-dsb-kiosk-lukker di Rubdown2837
Un'era presto finita: l'ultimo Kiosk DSB della Danimarca si chiude [DSBs deroute] - Centro Studi sulla Storia dell'Europa Orientale
Un'era presto finita: l'ultimo Kiosk DSB della Danimarca si chiude
www.csseo.org
July 22, 2025 at 6:51 AM
Un’era presto finita: l’ultimo Kiosk DSB della Danimarca si chiude [DSBs deroute]
https://www.csseo.org/794356/
Un’era presto finita: l’ultimo Kiosk DSB della Danimarca si chiude [DSBs deroute] https://avisendanmark.dk/danmark/en-epoke-snart-slut-danmarks-sidste-dsb-kiosk-lukker di Rubdown2837
https://www.csseo.org/794356/
Un’era presto finita: l’ultimo Kiosk DSB della Danimarca si chiude [DSBs deroute] https://avisendanmark.dk/danmark/en-epoke-snart-slut-danmarks-sidste-dsb-kiosk-lukker di Rubdown2837
We demonstrate that TDP1 catalytic mutations H263A and H493R (SCAN1), promote TOP1 cleavage complexes and TDP1-DNA covalent complexes respectively generating persistent TOP1-induced DSBs on quiescent cells. TDP1 mutants result in increased genome instability and reduced cell viability.
December 1, 2025 at 3:17 PM
We demonstrate that TDP1 catalytic mutations H263A and H493R (SCAN1), promote TOP1 cleavage complexes and TDP1-DNA covalent complexes respectively generating persistent TOP1-induced DSBs on quiescent cells. TDP1 mutants result in increased genome instability and reduced cell viability.
In mice and humans, DSBs occur almost exclusively where the protein PRDM9 binds DNA and makes two histone modifications, H3K4me3 and H3K36me3 (tinyurl.com/vjceh9wt). 4/n
February 23, 2024 at 7:02 PM
In mice and humans, DSBs occur almost exclusively where the protein PRDM9 binds DNA and makes two histone modifications, H3K4me3 and H3K36me3 (tinyurl.com/vjceh9wt). 4/n
Flere nordmenn tar beredskap alvorlig og lagrer bl.a. drikkevann i tråd med DSBs råd.
Men "Document"´s Kent Andersen skjønner ikke at det kan være så alvorlig. Nei, for terror og hybridkrig rammer ikke Norge. Ikke i tankeverdenen til "Document" og Kent.
Men "Document"´s Kent Andersen skjønner ikke at det kan være så alvorlig. Nei, for terror og hybridkrig rammer ikke Norge. Ikke i tankeverdenen til "Document" og Kent.
October 29, 2024 at 12:23 PM
Flere nordmenn tar beredskap alvorlig og lagrer bl.a. drikkevann i tråd med DSBs råd.
Men "Document"´s Kent Andersen skjønner ikke at det kan være så alvorlig. Nei, for terror og hybridkrig rammer ikke Norge. Ikke i tankeverdenen til "Document" og Kent.
Men "Document"´s Kent Andersen skjønner ikke at det kan være så alvorlig. Nei, for terror og hybridkrig rammer ikke Norge. Ikke i tankeverdenen til "Document" og Kent.
Our study shows a direct link between the circadian rhythm and the response to Repair of DNA double-strand breaks in transcribed genes, opening strategies for chrono-chemotherapies based on topoisomerase poisons that induce DSBs in active loci.
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Circadian PERIOD proteins regulate TC-DSB repair through anchoring to the nuclear envelope
Repair of DNA double-strand breaks (DSBs) produced in transcriptionally active chromatin occurs through a poorly characterized pathway called transcri…
www.sciencedirect.com
November 20, 2025 at 4:22 PM
Our study shows a direct link between the circadian rhythm and the response to Repair of DNA double-strand breaks in transcribed genes, opening strategies for chrono-chemotherapies based on topoisomerase poisons that induce DSBs in active loci.
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
These results support the potential role of TOP1-induced DSBs as a main contributor to certain hereditary neurological syndromes.
December 1, 2025 at 3:17 PM
These results support the potential role of TOP1-induced DSBs as a main contributor to certain hereditary neurological syndromes.
We also find L1-mediated DSBs can generate diverse types of large-scale intrachromosomal rearrangements, including deletions and duplications. Interestingly, we find L1 caused a pericentric ring chromosome!
December 19, 2024 at 9:52 PM
We also find L1-mediated DSBs can generate diverse types of large-scale intrachromosomal rearrangements, including deletions and duplications. Interestingly, we find L1 caused a pericentric ring chromosome!
DSBs remodel sister chromatids in cis and trans
Sister-pore-C allows us to track how DNA architecture changes before and after DSBs. We found that DSBs locally increase loops and interactions between the broken ends and the intact sister chromatid.
Sister-pore-C allows us to track how DNA architecture changes before and after DSBs. We found that DSBs locally increase loops and interactions between the broken ends and the intact sister chromatid.
February 12, 2025 at 5:15 PM
DSBs remodel sister chromatids in cis and trans
Sister-pore-C allows us to track how DNA architecture changes before and after DSBs. We found that DSBs locally increase loops and interactions between the broken ends and the intact sister chromatid.
Sister-pore-C allows us to track how DNA architecture changes before and after DSBs. We found that DSBs locally increase loops and interactions between the broken ends and the intact sister chromatid.
model where the reaction of oxygen is represented as the probability of inducing DNA strand breaks. Then it is integrated into a track-structure Monte Carlo simulation to investigate the impact of oxygen on the spatial distribution of DSBs within the [3/8 of https://arxiv.org/abs/2503.21837v1]
March 31, 2025 at 6:12 AM
model where the reaction of oxygen is represented as the probability of inducing DNA strand breaks. Then it is integrated into a track-structure Monte Carlo simulation to investigate the impact of oxygen on the spatial distribution of DSBs within the [3/8 of https://arxiv.org/abs/2503.21837v1]
"CRISPR/Cas9 system has recently been repurposed to enable target gene activation, allowing regulation of endogenous gene expression without creating [double-strand breaks] DSBs." #crispr #epigenetics #genome #geneediting
June 17, 2025 at 5:20 PM
"CRISPR/Cas9 system has recently been repurposed to enable target gene activation, allowing regulation of endogenous gene expression without creating [double-strand breaks] DSBs." #crispr #epigenetics #genome #geneediting