#AskZebrafish query from Slack: Looking for contact that has recently shipped transgenic eggs from Europe to the US. Re: any hurdles you or your lab had to go through. If this describes you or someone you know, please reach out 🙏
November 6, 2025 at 8:47 AM
#AskZebrafish query from Slack: Looking for contact that has recently shipped transgenic eggs from Europe to the US. Re: any hurdles you or your lab had to go through. If this describes you or someone you know, please reach out 🙏
Does anyone have examples of F32 supporting documents in the new format (starting August of this year) they'd be willing to share? We're specifically looking for goals, preparedness, training activities and timeline for an F32 resubmission for the December deadline. Any help very much appreciated! 🙏
November 2, 2025 at 4:45 PM
Query from Slack: Looking for people with experience in proximity labelling in zebrafish? Considering using miniTurbo instead of BioID or APEX, what do the community think of it? Also looking for anybody that has experience with targeting labeling enzymes to the ER/secretory pathway? #AskZebrafish
October 9, 2025 at 4:35 PM
Query from Slack: Looking for people with experience in proximity labelling in zebrafish? Considering using miniTurbo instead of BioID or APEX, what do the community think of it? Also looking for anybody that has experience with targeting labeling enzymes to the ER/secretory pathway? #AskZebrafish
zebrafish people @zebrafishrock.bsky.social the website for tol2kit seems to be down. is there a list online for IDs of plasmid #? for instance, I suspect #396 is template for transposase, but not sure! where can I find this list online in lieu of the kwan lab site?
January 27, 2025 at 1:01 PM
#AskZebrafish from Slack: “Does anyone have experience with ordering fish lines from the China Zebrafish Resource Center (CZRC)? My lab would like to order from their available resources but we’re unsure of what their international shipping process [to the US] entails. Any insights would be helpful”
November 27, 2024 at 5:10 PM
#AskZebrafish from Slack: “Does anyone have experience with ordering fish lines from the China Zebrafish Resource Center (CZRC)? My lab would like to order from their available resources but we’re unsure of what their international shipping process [to the US] entails. Any insights would be helpful”
🤔 #AskZebrafish: Looking for a transgenic line that labels cranial muscles, preferably in the USA? #zebrafish
May 9, 2025 at 10:37 AM
🤔 #AskZebrafish: Looking for a transgenic line that labels cranial muscles, preferably in the USA? #zebrafish
Query from Slack: Does anyone have a good way of ablating or preventing development of astrocytes in embryonic/larval zebrafish? Open to transgenic inducible lines, morpholinos, drugs, [maybe CRISPR], etc. #AskZebrafish
March 3, 2025 at 3:22 PM
Query from Slack: Does anyone have a good way of ablating or preventing development of astrocytes in embryonic/larval zebrafish? Open to transgenic inducible lines, morpholinos, drugs, [maybe CRISPR], etc. #AskZebrafish
Looking for anyone in the USA that has any of the new GCaMP8 fish lines and willing to share? 🤔 #AskZebrafish
April 7, 2025 at 5:40 PM
Looking for anyone in the USA that has any of the new GCaMP8 fish lines and willing to share? 🤔 #AskZebrafish
Does the perfect zebrafish labwork playlist exist? Esp for routine fishcare and breeding.
November 23, 2024 at 8:59 AM
ICYMI… We’ve got a dedicated handle for questions/queries called @askzebrafish.bsky.social. If you are ever bored and want to help the community, scroll thru their profile 🙏 #AskZebrafish
December 8, 2024 at 8:33 PM
ICYMI… We’ve got a dedicated handle for questions/queries called @askzebrafish.bsky.social. If you are ever bored and want to help the community, scroll thru their profile 🙏 #AskZebrafish
Anonymous #AskZebrafish: What is the best practice for generating a stable CRISPR/Cas9 knockout (i.e. to avoid genetic compensation)? Guides flanking either side of the gene? Around the promoter sequence? Use of an ssODN? Any advice would be thoroughly appreciated.
February 7, 2025 at 12:49 PM
Anonymous #AskZebrafish: What is the best practice for generating a stable CRISPR/Cas9 knockout (i.e. to avoid genetic compensation)? Guides flanking either side of the gene? Around the promoter sequence? Use of an ssODN? Any advice would be thoroughly appreciated.
One more query: Does anyone in the US have a Tg(myl7:Lifeact-FP) line and be willing to share embryos/adults? #AskZebrafish
March 27, 2025 at 5:55 PM
One more query: Does anyone in the US have a Tg(myl7:Lifeact-FP) line and be willing to share embryos/adults? #AskZebrafish
Another query from Slack: Does anyone (preferably in the US) have the Tg(GFAP:iGluSnFR) line and be willing to send them to another #zebrafish lab? 🤔 #AskZebrafish
March 27, 2025 at 3:39 PM
Another query from Slack: Does anyone (preferably in the US) have the Tg(GFAP:iGluSnFR) line and be willing to send them to another #zebrafish lab? 🤔 #AskZebrafish
#askzebrafish Does anyone have a good protocol for looking at antioxidant activity in larvae? I have a student interested in doing it for her honors thesis project but its not something we've ever done before.
November 22, 2024 at 2:37 PM
#askzebrafish Does anyone have a good protocol for looking at antioxidant activity in larvae? I have a student interested in doing it for her honors thesis project but its not something we've ever done before.
Another #AskZebrafish query: Does anyone know of a chemical/protocol to halt gut motility without affecting other smooth muscle organs? And without paralyzing the fish so that it can still gulp?
March 3, 2025 at 3:43 PM
Another #AskZebrafish query: Does anyone know of a chemical/protocol to halt gut motility without affecting other smooth muscle organs? And without paralyzing the fish so that it can still gulp?
Hmmmmmmmm just the Askzebrafish stuff? Why not?
November 16, 2024 at 7:41 PM
Hmmmmmmmm just the Askzebrafish stuff? Why not?
🤔 #AskZebrafish from Slack: Does anyone have the kdrl:mcherry plasmid and would be willing to send it to a lab in New Jersey, USA?
January 2, 2025 at 2:39 PM
🤔 #AskZebrafish from Slack: Does anyone have the kdrl:mcherry plasmid and would be willing to send it to a lab in New Jersey, USA?
Hi Bluesky 😀 Looking for advices on *how to improve my Filipin staining*. The tissue is only 3 cell layers tick. My goal is to stain the PM. If I do it live, cells dies. If I do it fixed (no permeabilization at all to keep the PM intact) it is too weak to makes conclusions. Any advices? Thanks!!
May 4, 2025 at 9:40 AM
Oi, stickleback researchers!
One of my PhD students is on a quest for lateral-view photographs of sticklebacks (like the one below, obvs not a stickleback). Any population/species, so long as the location and date of collection are known.
Does anyone know where we might find data like this?
One of my PhD students is on a quest for lateral-view photographs of sticklebacks (like the one below, obvs not a stickleback). Any population/species, so long as the location and date of collection are known.
Does anyone know where we might find data like this?
November 9, 2025 at 3:38 PM
Query from Slack Pro: Looking for WIK fish from a lab source within the EU and willing to share? If this describes your lab or someone you might know, please reach out 🙏 #AskZebrafish
May 26, 2025 at 1:01 PM
Query from Slack Pro: Looking for WIK fish from a lab source within the EU and willing to share? If this describes your lab or someone you might know, please reach out 🙏 #AskZebrafish
I love how the askzebrafish slack channel has organically morphed into a more serious guess that ISH
September 16, 2025 at 1:11 AM
I love how the askzebrafish slack channel has organically morphed into a more serious guess that ISH
🤔 Query from Slack: Looking for 5' myl7/cmlc2 promoter vector and someone willing to share it? Preferably in the US 🐟🫀 #AskZebrafish
June 20, 2025 at 7:14 PM
🤔 Query from Slack: Looking for 5' myl7/cmlc2 promoter vector and someone willing to share it? Preferably in the US 🐟🫀 #AskZebrafish
Query from our Slack Tankspace: Does anyone in Europe have the Tg(mpx: mcherry ) line from the Huttenlocher Lab or similar and would be willing to share? #AskZebrafish
April 4, 2025 at 2:42 PM
Query from our Slack Tankspace: Does anyone in Europe have the Tg(mpx: mcherry ) line from the Huttenlocher Lab or similar and would be willing to share? #AskZebrafish
Query from Slack: Does anyone know a reliable script/method to match piRNAs with TEs? (e.g. They would be interested to find piRNAs that target a particular TE) #AskZebrafish
November 13, 2024 at 8:18 AM
Query from Slack: Does anyone know a reliable script/method to match piRNAs with TEs? (e.g. They would be interested to find piRNAs that target a particular TE) #AskZebrafish
What are some advances in #zebrafish genetics post-2013? 🤔 #AskZebrafish
With a published genome & advancements in gene editing, the field of #zebrafish genetics has exploded in recent decades. We take a look back at major achievements in this infographic. For more, read this book chapter by @varshneylab.social et al (2015)($) doi.org/10.1016/bs.a... #ZebrafishFunFacts 🧪
May 10, 2025 at 12:37 PM
What are some advances in #zebrafish genetics post-2013? 🤔 #AskZebrafish