#ExpansionMicroscopy
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dimovalab.bsky.social
Helge @ewerslab.bsky.social knows not only how to organize conferences (#BiomembraneDays25) but also how septins organize. Exceptional imaging #ExpansionMicroscopy of cytokinesis
October 1, 2025 at 1:06 PM
franbottanelli.bsky.social
Who needs #clearing and #expansionmicroscopy?
rebeccarhelm.bsky.social Rebecca R Helm @rebeccarhelm.bsky.social · Dec 17
The glass amphipod Cystisoma is transparent because in the open ocean there is nowhere to hide, and its round head is completely covered in giant eyes. So when you're swimming far from shore just remember: you can't see them, but they can see you.
📽️ Dr. Alejandro Damian-Serrano 🧪🌊🌿🦑🐙
September 8, 2025 at 8:40 AM
plosbiology.org
#ExpansionMicroscopy allows multiple rounds of staining & imaging, but requires high-quality registration of images between rounds. This study develops a registration technique using dense NHS-ester labels for multiplexed cyclic imaging in expandable tissue gels @plosbiology.org 🧪 plos.io/44M4wcX
Registration of multiplexed images via actin staining in expanded cultured cells. (a) Composite image generated by registering two images acquired from the first and second imaging rounds. Phalloidin-labeled actin fibers were used as fiducial markers. The yellow box displays six-color images, including the DAPI channel. Gray, nucleus; red, actin; cyan, vimentin; blue, laminA/C; yellow, CCP; and magenta, cytokeratin 8/18. (b–c) Magnified views of the boxed region in a. Scale bars: (a) 2 µm; (b–c) 500 nm.
July 7, 2025 at 5:27 PM
plosbiology.org
#ExpansionMicroscopy allows multiple rounds of staining & imaging, but requires high-quality registration of images between rounds. This study develops a registration technique using dense NHS-ester labels for multiplexed cyclic imaging in expandable tissue gels @plosbiology.org 🧪 plos.io/44M4wcX
Registration of multiplexed images via actin staining in expanded cultured cells. (a) Composite image generated by registering two images acquired from the first and second imaging rounds. Phalloidin-labeled actin fibers were used as fiducial markers. The yellow box displays six-color images, including the DAPI channel. Gray, nucleus; red, actin; cyan, vimentin; blue, laminA/C; yellow, CCP; and magenta, cytokeratin 8/18. (b–c) Magnified views of the boxed region in a. Scale bars: (a) 2 µm; (b–c) 500 nm.
July 4, 2025 at 12:53 PM
plosbiology.org
#ExpansionMicroscopy allows multiple rounds of staining & imaging, but requires high-quality registration of images between rounds. This study develops a registration technique using dense NHS-ester labels for multiplexed cyclic imaging in expandable tissue gels @plosbiology.org 🧪 plos.io/44M4wcX
Registration of multiplexed images via actin staining in expanded cultured cells. (a) Composite image generated by registering two images acquired from the first and second imaging rounds. Phalloidin-labeled actin fibers were used as fiducial markers. The yellow box displays six-color images, including the DAPI channel. Gray, nucleus; red, actin; cyan, vimentin; blue, laminA/C; yellow, CCP; and magenta, cytokeratin 8/18. (b–c) Magnified views of the boxed region in a. Scale bars: (a) 2 µm; (b–c) 500 nm.
July 4, 2025 at 7:33 AM
mariezelena.bsky.social
Excited to give a talk next week at EMBO SRLM all about expansion microscopy! #expansionmicroscopy #ExM #EMBO
June 11, 2025 at 12:42 PM
dijiang319.bsky.social
@science.org Expansion in situ genome sequencing links nuclear abnormalities to aberrant chromatin regulation | Science www.science.org/doi/10.1126/...
@jbuenrostro.bsky.social @eboyden3.bsky.social + al @broadinstitute.org @harvard.edu
#ExIGS #Chromatin #Lamin #Progeria #Aging #ExpansionMicroscopy
Expansion in situ genome sequencing links nuclear abnormalities to aberrant chromatin regulation
Microscopy and genomics are used to characterize cell function, but approaches to connect the two types of information are lacking, particularly at subnuclear resolution. Here, we describe expansion i...
www.science.org
May 29, 2025 at 6:24 PM
louisefougere.bsky.social
🔬What's that ? 👋 Go see our last publication: ERGIC in plant cell.
@yohannboutte.bsky.social @bic-bordeaux.bsky.social @cnrsbiologie.bsky.social @lbm-bordeaux.bsky.social @naturecellbiology.bsky.social
#tau-STED #ExpansionMicroscopy #CellBiology #PlantCell
February 25, 2025 at 10:40 AM
yohannboutte.bsky.social
Super-resolution microscopy unravels a dynamic tubular network that is stabilized by pre-existing Golgi and lipids to mature into new Golgi pre-cisternae, @bic-bordeaux.bsky.social, @cnrsbiologie.bsky.social, @cnrsaquitaine.bsky.social, #Lipids, #ExpansionMicroscopy, #PlantScience, #PlantCellBiology
February 25, 2025 at 10:11 AM
monica-bic.bsky.social
Great picture of expansion procedure!!! Thanks and welcome!!!#ExpansionMicroscopy #ExM
sventruckenbrodt.bsky.social Sven Truckenbrodt @sventruckenbrodt.bsky.social · Feb 15
I don't think I'm ever going to get tired of expansion microscopy, so this makes for a great first post here to look back on in the years to come 😄

Mouse brain slices, unexpanded and at two different expansion levels, skillfully prepared by @damstra.bsky.social and Aashir Meeran at E11 Bio! 🧠
Mouse brain slices, unexpanded and at two different expansion levels, prepared using expansion microscopy.
February 15, 2025 at 9:13 AM
moffittlab.bsky.social
However, three orders of magnitude didn’t seem so daunting to Ari, Yuanyou, and Nana, so they built a bacteria-optimized #ExpansionMicroscopy toolbox that is capable of tunable expansion of individual bacteria up to 1000-fold in volume! 5/
February 2, 2025 at 3:16 PM
sonhita.bsky.social
🌱🔬Excited to share my recent In Brief article published in @theplantcell.bsky.social @aspbofficial.bsky.social 🧪🌾

🔗 doi.org/10.1093/plce...

I enjoyed covering this #ThePlantCell paper and I look forward to seeing #PlantEx benefit the #PlantScience community.

#PlantBiology #ExpansionMicroscopy
Figure. PlantEx at a glance. A) During PlantEx Arabidopsis seedlings (1) are immunolabelled and primed with anchoring groups (1), are treated with hydrogel solution to enable polymerization with anchoring agents (2), have their cell walls digested and protein cohesiveness disrupted via homogenization (3,4) and physically expand to improve resolution (5). B) Dual-color confocal imaging of lateral root cap cells show COPI-coated vesicles (γ-COP, sec21, green) and trans-Golgi network (VHA-a1, magenta) pre- (left) and post-expansion (right). Adapted from Gallei et al. (2024), Figures 1, 3.
January 27, 2025 at 1:28 PM
kristenseveri.bsky.social
Very happy to share our latest, a review on #ExpansionMicroscopy across organisms (including zebrafish, mice, Drosophila, and C. elegans) with @galhaspel.bsky.social and more :) #zebrafish #microscopy doi.org/10.1117/1.NP...
Expansion microscopy reveals neural circuit organization in genetic animal models
Expansion microscopy is a super-resolution technique in which physically enlarging the samples in an isotropic manner increases inter-molecular distances such that nano-scale structures can be resolve...
doi.org
December 20, 2024 at 6:24 PM
ksenart.bsky.social
My '37th View of Mount Fuji,' is a January cover for @scotmicro's 2025 fundraising calendar!
Got it at SMS 50th Annual Symposium, where I gave a talk on #expansionmicroscopy :)
A 'mountain' formed by ependymal cell nuclei in the mouse brainstem.
#Microscopy #Neuroscience #Brain #FluorescenceFriday
December 20, 2024 at 2:10 PM
dyneinassembly.bsky.social
This was such a great meeting. I would highly recommend joining this friendly network of cilia (AND drum-roll please!) centrosome enthusiats. The network is expanding #expansionmicroscopy lol!
ukcilianetwork.bsky.social UK Cilia and Centrosome Network @ukcilianetwork.bsky.social · Nov 25
UK Cilia and Centrosome Network autumn meeting in Liverpool was a success!
1) Great ECR representation+diveristy✓
2) Talks from leading experts inc. EMBO-YIP Elif @cytolabkoc.bsky.social ✓
3) Superb networking opportunities ✓
Thanks to Ula @urszulamcclurg.bsky.social for pulling it all together
November 25, 2024 at 10:46 AM
somssich.bsky.social
📜 ExPOSE: A comprehensive toolkit to perform expansion microscopy in plant protoplast systems

🧑‍🔬 Kevin L. Cox, Sarah A. Pardi, Kirk J. Czymmek, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ #PlantScience #PlantMicroscopy #ExpansionMicroscopy #PlantMethods
ExPOSE: A comprehensive toolkit to perform expansion microscopy in plant protoplast systems
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
www.biorxiv.org
July 26, 2024 at 7:37 AM
somssich.bsky.social
Ok, so #ExpansionMicroscopy has now reached the #PlantScience field 🔬🌾

Preprints from two labs out today on @biorxiv-plants.bsky.social

#1, Magali S. Grison et al.
bsky.app/profile/soms...

#2: Michelle Gallei, Sven Truckenbrodt, et al.
bsky.app/profile/soms...

#PlantMicroscopy #PlantCellBiology
February 21, 2024 at 2:25 PM
somssich.bsky.social
📜 Super-resolution expansion microscopy in plant roots

🧑‍🔬 Michelle Gallei, Sven Truckenbrodt, Johann G. Danzl, et al.

📔 @biorxiv-plants.bsky.social

🔗 www.biorxiv.org/content/10.1...

#️⃣ (Feeds) #PlantScience #PlantMicroscopy #ExpansionMicroscopy #PlantCellBiology
Super-resolution expansion microscopy in plant roots
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
www.biorxiv.org
February 21, 2024 at 12:32 PM
haoyin.bsky.social
"Cx43 plaques as potential keratinocyte-nerve fiber communication sites" in👤Skin
#SuperResolutionArrayTomography
#ExpansionMicroscopy

+Coculture hiPSC-sensory neuron + primary keratinocyte to model physical ensheathment & Ca2+ coupling

#eLife 2024
elifesciences.org/articles/77761
January 26, 2024 at 1:13 AM
kathaschmack.bsky.social
🤯 A conventional light microscope can now see a single synaptic vesicle! The shape of antibodies! Conformational changes of calmodulin!

With a new #ExpansionMicroscopy method👇 Great times for curious minds to be alive!

x.com/AliHShaib/stat…
February 10, 2025 at 9:22 PM
christletx.bsky.social
Nice #ExpansionMicroscopy showing astrocyte contacts with synapses in brain sections https://www.cell.com/cell-reports/fulltext/S2211-1247(20)31171-2
January 2, 2025 at 2:40 PM