Big thanks to the University of York, with their Glacios which we used for screening and another big thanks to eBIC which was used to do the larger data collections that produced our reported structures. Without these facilities our cryo-EM studies would have been far more difficult.
November 11, 2025 at 1:25 PM
Big thanks to the University of York, with their Glacios which we used for screening and another big thanks to eBIC which was used to do the larger data collections that produced our reported structures. Without these facilities our cryo-EM studies would have been far more difficult.
Douglas also collected cryo-EM data of MTR with hydroxycobalamin which does not support activity and methylcobalamin which is the active form of the cofactor. Though MTR again behaves as two separate bodies, we were able to observe conformational changes due to cobalamin binding.
November 11, 2025 at 1:10 PM
Douglas also collected cryo-EM data of MTR with hydroxycobalamin which does not support activity and methylcobalamin which is the active form of the cofactor. Though MTR again behaves as two separate bodies, we were able to observe conformational changes due to cobalamin binding.
Due to its flexibility MTR is a tricky protein to study by classical structural techniques however cryo-EM is well suited for this type of sample. Douglas could successfully purify full-length MTR which allowed him to determine two separate classes of MTR without cbl.
November 11, 2025 at 1:03 PM
Due to its flexibility MTR is a tricky protein to study by classical structural techniques however cryo-EM is well suited for this type of sample. Douglas could successfully purify full-length MTR which allowed him to determine two separate classes of MTR without cbl.
Our brilliant @louieaspinall.bsky.social and Tom are representing our Facility at #PSDI in Basel sharing how Cryo-EM at Leeds can support your drug discovery projects.
If you’re at the conference, come say hi before it wraps up!! 👋
If you’re at the conference, come say hi before it wraps up!! 👋
November 11, 2025 at 11:13 AM
Our brilliant @louieaspinall.bsky.social and Tom are representing our Facility at #PSDI in Basel sharing how Cryo-EM at Leeds can support your drug discovery projects.
If you’re at the conference, come say hi before it wraps up!! 👋
If you’re at the conference, come say hi before it wraps up!! 👋
Structural insight into glycosyltransferase function
ARC Centre for Cryo-EM of Membrane Proteins
youtu.be/jPy4l4M-xQ4?...
ARC Centre for Cryo-EM of Membrane Proteins
youtu.be/jPy4l4M-xQ4?...
Structural insight into glycosyltransferase function
YouTube video by ARC Centre for Cryo-EM of Membrane Proteins
youtu.be
November 11, 2025 at 9:38 AM
Structural insight into glycosyltransferase function
ARC Centre for Cryo-EM of Membrane Proteins
youtu.be/jPy4l4M-xQ4?...
ARC Centre for Cryo-EM of Membrane Proteins
youtu.be/jPy4l4M-xQ4?...
My first work in solving SLC transporters using cryo-EM without any fiducial markers. (Nature communications, 2022)
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Cryo-EM structure of human glucose transporter GLUT4 - Nature Communications
Small solute carriers remain difficult to study by single particle cryo-EM. Here, the authors report the cryo-EM structure of human insulin-responsive glucose transporter GLUT4 (55 kDa) without rigid ...
www.nature.com
November 11, 2025 at 9:31 AM
My first work in solving SLC transporters using cryo-EM without any fiducial markers. (Nature communications, 2022)
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Cryo-EM structures of the BAM-P2-visible SurA complex reveal dynamic and cooperative interactions in outer membrane protein assembly www.biorxiv.org/content/10.1101/2025.11.10.687524v1 #cryoem
November 11, 2025 at 8:10 AM
Cryo-EM structures of the BAM-P2-visible SurA complex reveal dynamic and cooperative interactions in outer membrane protein assembly www.biorxiv.org/content/10.1101/2025.11.10.687524v1 #cryoem
Cryo-EM structures of the BAM-P2-visible SurA complex reveal dynamic and cooperative interactions in outer membrane protein assembly https://www.biorxiv.org/content/10.1101/2025.11.10.687524v1
November 11, 2025 at 2:17 AM
Cryo-EM structures of the BAM-P2-visible SurA complex reveal dynamic and cooperative interactions in outer membrane protein assembly https://www.biorxiv.org/content/10.1101/2025.11.10.687524v1
Cryo-EM structures of the BAM-P2-visible SurA complex reveal dynamic and cooperative interactions in outer membrane protein assembly https://www.biorxiv.org/content/10.1101/2025.11.10.687524v1
November 11, 2025 at 2:17 AM
Cryo-EM structures of the BAM-P2-visible SurA complex reveal dynamic and cooperative interactions in outer membrane protein assembly https://www.biorxiv.org/content/10.1101/2025.11.10.687524v1
#MD blind predictions of water/ions around Tetrahymena ribozyme #RNA matched cryo-EM data better than our baselines! Congrats #CASP16 + @rachael-kretsch.bsky.social + 23 brave groups. Still a ways to go — time for #DeepLearning of water? Community paper: doi.org/10.1002/prot...
November 11, 2025 at 12:53 AM
#MD blind predictions of water/ions around Tetrahymena ribozyme #RNA matched cryo-EM data better than our baselines! Congrats #CASP16 + @rachael-kretsch.bsky.social + 23 brave groups. Still a ways to go — time for #DeepLearning of water? Community paper: doi.org/10.1002/prot...
Using Leishmania as a model, we determined the cryo-EM structure of the doublet microtubule to pinpoint the position of each dynein. This gave us a detailed map of where every “rower” sits on the ciliary “boat.”
November 10, 2025 at 5:45 PM
Using Leishmania as a model, we determined the cryo-EM structure of the doublet microtubule to pinpoint the position of each dynein. This gave us a detailed map of where every “rower” sits on the ciliary “boat.”
New Title Alert: ROCKET- is a tool for refining AlphaFold predictions using crystallographic and cryo-EM likelihood targets.
Learn more here: buff.ly/s9y4bFV
#SBGrid #SBGridSoftware #StructuralBiology
Learn more here: buff.ly/s9y4bFV
#SBGrid #SBGridSoftware #StructuralBiology
GitHub - alisiafadini/ROCKET
Contribute to alisiafadini/ROCKET development by creating an account on GitHub.
buff.ly
November 10, 2025 at 5:30 PM
New Title Alert: ROCKET- is a tool for refining AlphaFold predictions using crystallographic and cryo-EM likelihood targets.
Learn more here: buff.ly/s9y4bFV
#SBGrid #SBGridSoftware #StructuralBiology
Learn more here: buff.ly/s9y4bFV
#SBGrid #SBGridSoftware #StructuralBiology
Researchers from Zhejiang University and UC Davis studied how metformin works differently from other biguanides.
Using cryo-EM, they found metformin enters complex I only when it is open and blocks CoQ10 in the closed state, revealing why it’s safer and effective.
www.nature.com/articles/s41...
Using cryo-EM, they found metformin enters complex I only when it is open and blocks CoQ10 in the closed state, revealing why it’s safer and effective.
www.nature.com/articles/s41...
Hydrophilic metformin and hydrophobic biguanides inhibit mitochondrial complex I by distinct mechanisms - Nature Structural & Molecular Biology
He, Teng and Yang et al. report how metformin, the widely used antidiabetic drug, inhibit its target, the respiratory complex I, through a distinct state-dependent, inhibitor trapping mechanism, thus ...
www.nature.com
November 10, 2025 at 12:53 PM
Researchers from Zhejiang University and UC Davis studied how metformin works differently from other biguanides.
Using cryo-EM, they found metformin enters complex I only when it is open and blocks CoQ10 in the closed state, revealing why it’s safer and effective.
www.nature.com/articles/s41...
Using cryo-EM, they found metformin enters complex I only when it is open and blocks CoQ10 in the closed state, revealing why it’s safer and effective.
www.nature.com/articles/s41...
🇨🇭 Meet Roman at #PSDI2025 (poster PO21) to see how our tools reveal drug-induced conformational changes, bringing static structures to life. Then 🇦🇹 join him & Tim at the 8th Austrian Cryo-EM Symposium. DM us to connect! #cryoem #drugdiscovery #structuralbiology
November 10, 2025 at 8:22 AM
🇨🇭 Meet Roman at #PSDI2025 (poster PO21) to see how our tools reveal drug-induced conformational changes, bringing static structures to life. Then 🇦🇹 join him & Tim at the 8th Austrian Cryo-EM Symposium. DM us to connect! #cryoem #drugdiscovery #structuralbiology
New! The teams of Simon Boulton and Steve West combined cryo-EM, biochemical assays and the C-Trap to "define the structure and function of a previously uncharacterized complex in the context of DNA double-strand break repair, and replication fork stabilization and restart".
bit.ly/43RG6xU
bit.ly/43RG6xU
Cryo–electron microscopy visualization of RAD51 filament assembly and end-capping by XRCC3-RAD51C-RAD51D-XRCC2
Homologous recombination repairs DNA double strand breaks and protects stalled replication forks, but how the five RAD51 paralogs contribute to these processes remains unclear. Mutations in the RAD51 ...
www.science.org
November 10, 2025 at 8:22 AM
New! The teams of Simon Boulton and Steve West combined cryo-EM, biochemical assays and the C-Trap to "define the structure and function of a previously uncharacterized complex in the context of DNA double-strand break repair, and replication fork stabilization and restart".
bit.ly/43RG6xU
bit.ly/43RG6xU
Cryo-EM reveals multiple mechanisms of ribosome inhibition by doxycycline www.biorxiv.org/content/10.1101/2025.11.08.686982v1 #cryoem
November 10, 2025 at 8:11 AM
Cryo-EM reveals multiple mechanisms of ribosome inhibition by doxycycline www.biorxiv.org/content/10.1101/2025.11.08.686982v1 #cryoem
Cryo-EM reveals multiple mechanisms of ribosome inhibition by doxycycline https://www.biorxiv.org/content/10.1101/2025.11.08.686982v1
November 10, 2025 at 3:46 AM
Cryo-EM reveals multiple mechanisms of ribosome inhibition by doxycycline https://www.biorxiv.org/content/10.1101/2025.11.08.686982v1
Cryo-EM reveals multiple mechanisms of ribosome inhibition by doxycycline https://www.biorxiv.org/content/10.1101/2025.11.08.686982v1
November 10, 2025 at 3:46 AM
Cryo-EM reveals multiple mechanisms of ribosome inhibition by doxycycline https://www.biorxiv.org/content/10.1101/2025.11.08.686982v1
Congratulations to all authors! Happy to have contributed to the cryo-EM side. It was a lot of fun learning more about the herpesvirus DNA replication process and how clinical drugs inhibit it 🦠💊. Big thanks to @cijilim.bsky.social and Tahir for the support!
New work from Tahirov x Lim collaboration! @qixianghe.bsky.social from my lab contributed the cryo-EM structures for this work. We are excited to help explain how anti-HSV drugs work and to guide their future development.
www.science.org/doi/10.1126/...
@unmc.bsky.social @uwbiochem.bsky.social
www.science.org/doi/10.1126/...
@unmc.bsky.social @uwbiochem.bsky.social
Structural basis of herpesvirus helicase-primase inhibition by pritelivir and amenamevir
Structural studies of HSV-1 helicase-primase revealed how pritelivir and amenamevir bind and block its helicase activity.
www.science.org
November 10, 2025 at 2:49 AM
Congratulations to all authors! Happy to have contributed to the cryo-EM side. It was a lot of fun learning more about the herpesvirus DNA replication process and how clinical drugs inhibit it 🦠💊. Big thanks to @cijilim.bsky.social and Tahir for the support!
New work from Tahirov x Lim collaboration! @qixianghe.bsky.social from my lab contributed the cryo-EM structures for this work. We are excited to help explain how anti-HSV drugs work and to guide their future development.
www.science.org/doi/10.1126/...
@unmc.bsky.social @uwbiochem.bsky.social
www.science.org/doi/10.1126/...
@unmc.bsky.social @uwbiochem.bsky.social
Structural basis of herpesvirus helicase-primase inhibition by pritelivir and amenamevir
Structural studies of HSV-1 helicase-primase revealed how pritelivir and amenamevir bind and block its helicase activity.
www.science.org
November 10, 2025 at 12:14 AM
New work from Tahirov x Lim collaboration! @qixianghe.bsky.social from my lab contributed the cryo-EM structures for this work. We are excited to help explain how anti-HSV drugs work and to guide their future development.
www.science.org/doi/10.1126/...
@unmc.bsky.social @uwbiochem.bsky.social
www.science.org/doi/10.1126/...
@unmc.bsky.social @uwbiochem.bsky.social
SuperFrame Cryo, 3 g, 9 W/(m·K)
R$ 19,90
Avaliação: ⭐⭐⭐⭐⭐ 🥉
Loja: Terabyte
Menor preço em 90 dias: R$ 19,90
Mediana dos preços de 90 dias: R$ 19,90
R$ 19,90
Avaliação: ⭐⭐⭐⭐⭐ 🥉
Loja: Terabyte
Menor preço em 90 dias: R$ 19,90
Mediana dos preços de 90 dias: R$ 19,90
November 9, 2025 at 2:01 PM
SuperFrame Cryo, 3 g, 9 W/(m·K)
R$ 19,90
Avaliação: ⭐⭐⭐⭐⭐ 🥉
Loja: Terabyte
Menor preço em 90 dias: R$ 19,90
Mediana dos preços de 90 dias: R$ 19,90
R$ 19,90
Avaliação: ⭐⭐⭐⭐⭐ 🥉
Loja: Terabyte
Menor preço em 90 dias: R$ 19,90
Mediana dos preços de 90 dias: R$ 19,90
Cryo-EM Structure of FcεRI Bound IgE Reveals Multiple Defined Conformations of the Fab-Fc Hinge pubmed.ncbi.nlm.nih.gov/41199488/ #cryoem
November 9, 2025 at 4:52 AM
Cryo-EM Structure of FcεRI Bound IgE Reveals Multiple Defined Conformations of the Fab-Fc Hinge pubmed.ncbi.nlm.nih.gov/41199488/ #cryoem
CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection pubmed.ncbi.nlm.nih.gov/41201048/ #cryoem
November 9, 2025 at 2:34 AM
CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection pubmed.ncbi.nlm.nih.gov/41201048/ #cryoem
Jan-Hannes Schäfer et al.: CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection ##Automation##DataProcessing##CryoEM@umich...##IUCrhttps://journals.iucr.org/paper?S2053230X25008866
CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection
CryoSift is a CNN-based tool that assesses the quality of 2D class averages. The incorporation of CryoSift into cryoSPARC workflows enables automated particle-stack cleaning.
journals.iucr.org
November 8, 2025 at 1:00 AM
Jan-Hannes Schäfer et al.: CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection ##Automation##DataProcessing##CryoEM@umich...##IUCrhttps://journals.iucr.org/paper?S2053230X25008866
Announcing cryo-EM heterogeneity challenge #2, now dubbed the 2025 Community-Wide Assessment of Cryo-EM Heterogeneous Reconstruction Algorithms (CAHRA)! Join us for a webinar next Friday (Nov 14th) to learn more. Datasets already posted here: heterogeneity.notion.site/challenge #cryoem
CAHRA 2025: Community-Wide Assessment of Cryo-EM Heterogeneous Reconstruction Algorithms
A community-wide data processing challenge for cryo-electron microscopy.
heterogeneity.notion.site
November 7, 2025 at 10:45 PM
Announcing cryo-EM heterogeneity challenge #2, now dubbed the 2025 Community-Wide Assessment of Cryo-EM Heterogeneous Reconstruction Algorithms (CAHRA)! Join us for a webinar next Friday (Nov 14th) to learn more. Datasets already posted here: heterogeneity.notion.site/challenge #cryoem