Next-generation enhancer AAVs for selective interspecies targeting of midbrain dopaminergic neurons https://www.biorxiv.org/content/10.1101/2025.11.06.687071v1
November 8, 2025 at 7:15 AM
Next-generation enhancer AAVs for selective interspecies targeting of midbrain dopaminergic neurons https://www.biorxiv.org/content/10.1101/2025.11.06.687071v1
AAVs are crucial vectors for delivering biosensors into neurons! Using AAV delivery, we perform two-color imaging of calcium and potassium transients in neurons. These AAVs are now available from @braincase.bsky.social www.ebraincase.com/news/new-pro...
November 6, 2025 at 10:15 AM
AAVs are crucial vectors for delivering biosensors into neurons! Using AAV delivery, we perform two-color imaging of calcium and potassium transients in neurons. These AAVs are now available from @braincase.bsky.social www.ebraincase.com/news/new-pro...
#FluorescenceFriday 🌿🔬 post:
Avian retina transduced with my AAVs carrying the #GluSNFR biosensor.
Bright blobs at the top mark photoreceptor terminals; meanwhile below, an RGC connects beautifully with bipolar cells, giving a glimpse of retinal circuitry in action.
#Retina #Neuroscience #AAV #2-P
Avian retina transduced with my AAVs carrying the #GluSNFR biosensor.
Bright blobs at the top mark photoreceptor terminals; meanwhile below, an RGC connects beautifully with bipolar cells, giving a glimpse of retinal circuitry in action.
#Retina #Neuroscience #AAV #2-P
October 24, 2025 at 7:10 PM
#FluorescenceFriday 🌿🔬 post:
Avian retina transduced with my AAVs carrying the #GluSNFR biosensor.
Bright blobs at the top mark photoreceptor terminals; meanwhile below, an RGC connects beautifully with bipolar cells, giving a glimpse of retinal circuitry in action.
#Retina #Neuroscience #AAV #2-P
Avian retina transduced with my AAVs carrying the #GluSNFR biosensor.
Bright blobs at the top mark photoreceptor terminals; meanwhile below, an RGC connects beautifully with bipolar cells, giving a glimpse of retinal circuitry in action.
#Retina #Neuroscience #AAV #2-P
Very happy to share an expansion to the comparative gene editing toolkit: AAVs to reduce dopamine receptor levels that are functional in many rodent species. Fun collaboration with @malu-murugan.bsky.social, Elliott Albers and Frank Meye.
Comparative gene editing reduces dopamine receptor levels across rodent species
Translational challenges in neuroscience originate from species-specific differences that limit the generalizability of experimental findings. Comparative approaches can help distinguish conserved fro...
www.biorxiv.org
October 22, 2025 at 5:59 PM
Very happy to share an expansion to the comparative gene editing toolkit: AAVs to reduce dopamine receptor levels that are functional in many rodent species. Fun collaboration with @malu-murugan.bsky.social, Elliott Albers and Frank Meye.
Another big deal: the Ducks are making Jackson LaCombe the highest-paid player in franchise history. An 8x$9M AAV extension for the talented young defenceman.
Ryan Getzlaf, Paul Kariya and Corey Perry had higher AAVs, but this is the most in total dollars.
[Response to a quoted post]
Ryan Getzlaf, Paul Kariya and Corey Perry had higher AAVs, but this is the most in total dollars.
[Response to a quoted post]
nitter.poast.org
October 2, 2025 at 5:57 PM
Another big deal: the Ducks are making Jackson LaCombe the highest-paid player in franchise history. An 8x$9M AAV extension for the talented young defenceman.
Ryan Getzlaf, Paul Kariya and Corey Perry had higher AAVs, but this is the most in total dollars.
[Response to a quoted post]
Ryan Getzlaf, Paul Kariya and Corey Perry had higher AAVs, but this is the most in total dollars.
[Response to a quoted post]
I think they should just take this to 11 on the batshit crazy scale. Air Force generals must fly in. Marine generals must land in AAVs. Navy can sail up the Chesapeake in a 18 century ship of the line. Army generals will arrive at the Quantico triangle gate in caissons
Top generals and admirals are scrambling to find aircraft and make other last-minute plans to attend Defense Secretary Pete Hegseth’s unusual meeting.
Generals and admirals scrambling to make plans to attend Hegseth's unusual meeting
The meeting is notable for its size and timing, and for the fact that Defense Secretary Pete Hegseth has ordered his entire senior officer corps to Washington on short notice.
nbcnews.to
September 27, 2025 at 8:34 AM
I think they should just take this to 11 on the batshit crazy scale. Air Force generals must fly in. Marine generals must land in AAVs. Navy can sail up the Chesapeake in a 18 century ship of the line. Army generals will arrive at the Quantico triangle gate in caissons
If you use AAVs in your research, you'll want to check out the latest additions to Addgene's viral vector service! See them all in our latest blog post, including the first vector packaged in a new-to-Addgene serotype, AAV9-X1.1.
blog.addgene.org/new...
blog.addgene.org/new...
New Viral Vectors - Fall 2024
Addgene adds new ready-to-use viral vectors to our service every month. Read all about the latest additions here!
blog.addgene.org
October 3, 2024 at 2:09 PM
If you use AAVs in your research, you'll want to check out the latest additions to Addgene's viral vector service! See them all in our latest blog post, including the first vector packaged in a new-to-Addgene serotype, AAV9-X1.1.
blog.addgene.org/new...
blog.addgene.org/new...
Zack Martin has a point imo. He's the 31st highest-paid lineman in the league. Seven guards have higher contract AAVs than him. You can't convince me there are seven guards better than him at what he does.
December 1, 2024 at 10:10 AM
Zack Martin has a point imo. He's the 31st highest-paid lineman in the league. Seven guards have higher contract AAVs than him. You can't convince me there are seven guards better than him at what he does.
Like you can do this all day. You have to draw a line somewhere and it's always going to feel arbitrary. The Leafs need to stop acting like they've got nothing to offer a player besides above market AAVs.
November 26, 2024 at 5:02 AM
Like you can do this all day. You have to draw a line somewhere and it's always going to feel arbitrary. The Leafs need to stop acting like they've got nothing to offer a player besides above market AAVs.
3/9 The technique: Non-disease-causing adeno-associated viruses (AAVs) deliver genes to specific neurons. Scientists attach genetic 'enhancers' to gutted AAVs, fill with target genes. Viral shuttles bypass blood-brain barrier, activate only in target cells.
#GeneTherapy
#GeneTherapy
June 5, 2025 at 6:09 AM
3/9 The technique: Non-disease-causing adeno-associated viruses (AAVs) deliver genes to specific neurons. Scientists attach genetic 'enhancers' to gutted AAVs, fill with target genes. Viral shuttles bypass blood-brain barrier, activate only in target cells.
#GeneTherapy
#GeneTherapy
High-quality AAVs for red genetically encoded calcium indicator FRCaMPi are now available from BrainCase anywhere in the world www.ebraincase.com/products-ser...
October 23, 2025 at 2:02 AM
High-quality AAVs for red genetically encoded calcium indicator FRCaMPi are now available from BrainCase anywhere in the world www.ebraincase.com/products-ser...
and maximum sidelobe level to avoid information leakage from the known and unknown eavesdroppers, respectively. Simultaneously, we also minimize the energy consumption of AAVs when constructing virtual antenna arrays. Due to [3/9 of https://arxiv.org/abs/2503.00721v1]
March 4, 2025 at 5:59 AM
and maximum sidelobe level to avoid information leakage from the known and unknown eavesdroppers, respectively. Simultaneously, we also minimize the energy consumption of AAVs when constructing virtual antenna arrays. Due to [3/9 of https://arxiv.org/abs/2503.00721v1]
AAVS Helps Chimps with $100,000 Gift
AAVS Helps Chimps with $100,000 Gift
www.vegan-news.net
March 7, 2025 at 6:08 PM
AAVS Helps Chimps with $100,000 Gift
Approved/early gene therapies are very expensive, driven by small-scale and unoptimized production and small target populations to recoup R&D expenses. At safe doses and millions of patients, AAVs can be priced similar to one year of antibody drugs, but with long-term effects.
March 20, 2025 at 2:29 PM
Approved/early gene therapies are very expensive, driven by small-scale and unoptimized production and small target populations to recoup R&D expenses. At safe doses and millions of patients, AAVs can be priced similar to one year of antibody drugs, but with long-term effects.
This work is now online in the latest issue of Neuron. At the Broad Institute of MIT and Harvard, the team has developed enhancer-AAVs to target diverse cortical interneuron subtypes
www.cell.com/neuron/fullt...
#neuron #neuroscience #harvard
www.cell.com/neuron/fullt...
#neuron #neuroscience #harvard
May 25, 2025 at 5:09 PM
This work is now online in the latest issue of Neuron. At the Broad Institute of MIT and Harvard, the team has developed enhancer-AAVs to target diverse cortical interneuron subtypes
www.cell.com/neuron/fullt...
#neuron #neuroscience #harvard
www.cell.com/neuron/fullt...
#neuron #neuroscience #harvard
This has immediate utility as a research toolbox and longer-term value as an approach for gene therapies. This is part of the Allen Institute's larger effort to create enhancer AAVs toolboxes across several brain regions.
July 8, 2025 at 8:08 PM
This has immediate utility as a research toolbox and longer-term value as an approach for gene therapies. This is part of the Allen Institute's larger effort to create enhancer AAVs toolboxes across several brain regions.
See How They Grow – an AAVS Valentine
See How They Grow – an AAVS Valentine
www.vegan-news.net
February 13, 2025 at 9:30 PM
See How They Grow – an AAVS Valentine
Important PSA / technical note for any using AAVs for experiments that rely on linked elements/barcodes (e.g. multiplexed reporters, MPRAs, certain single cell CRISPR screens).
In work led by @jblalanne.bsky.social - we saw AAVs unlink/swap barcodes when packaged as a pool - design aware!
In work led by @jblalanne.bsky.social - we saw AAVs unlink/swap barcodes when packaged as a pool - design aware!
Brief PSA for the functional genomics community: if you are profiling complex libraries with >1 internal components (e.g., enhancer+barcodes in MPRAs) delivered via AAVs, be aware that there might be substantial unlinking within your libraries if you packaged as a pool! doi.org/10.1101/2025...
February 14, 2025 at 8:42 PM
Important PSA / technical note for any using AAVs for experiments that rely on linked elements/barcodes (e.g. multiplexed reporters, MPRAs, certain single cell CRISPR screens).
In work led by @jblalanne.bsky.social - we saw AAVs unlink/swap barcodes when packaged as a pool - design aware!
In work led by @jblalanne.bsky.social - we saw AAVs unlink/swap barcodes when packaged as a pool - design aware!
1/9
🦟 ENGINEERED VIRUSES MAKE NEURONS GLOW AND TREAT BRAIN DISEASE 🦟
Scientists from 29 institutions found 1,000+ new ways to target specific brain cells using engineered viruses (AAVs). They can turn neurons off, activate them, or 'light them up.'
#Neuroscience
🦟 ENGINEERED VIRUSES MAKE NEURONS GLOW AND TREAT BRAIN DISEASE 🦟
Scientists from 29 institutions found 1,000+ new ways to target specific brain cells using engineered viruses (AAVs). They can turn neurons off, activate them, or 'light them up.'
#Neuroscience
June 5, 2025 at 6:07 AM
1/9
🦟 ENGINEERED VIRUSES MAKE NEURONS GLOW AND TREAT BRAIN DISEASE 🦟
Scientists from 29 institutions found 1,000+ new ways to target specific brain cells using engineered viruses (AAVs). They can turn neurons off, activate them, or 'light them up.'
#Neuroscience
🦟 ENGINEERED VIRUSES MAKE NEURONS GLOW AND TREAT BRAIN DISEASE 🦟
Scientists from 29 institutions found 1,000+ new ways to target specific brain cells using engineered viruses (AAVs). They can turn neurons off, activate them, or 'light them up.'
#Neuroscience
I’m surprised how low the AAVs have been for all of the Bucks’ signings. Hopefully means something good’s coming?
July 1, 2025 at 1:54 AM
I’m surprised how low the AAVs have been for all of the Bucks’ signings. Hopefully means something good’s coming?
Thoughts on this? >> A Suite of Enhancer AAVs Support Targeted Gene Therapy for
Brain Disease - Genetic Engineering and Biotechnology News >> Comment below! #strategy #competitiveintelligence #marketing #healthcare #pharmaceutical #biotech #pharma #competitivemarketing
Brain Disease - Genetic Engineering and Biotechnology News >> Comment below! #strategy #competitiveintelligence #marketing #healthcare #pharmaceutical #biotech #pharma #competitivemarketing
A Suite of Enhancer AAVs Support Targeted Gene Therapy for
Brain Disease - Genetic Engineering and Biotechnology News
A Suite of Enhancer AAVs Support Targeted Gene Therapy for Brain Disease Genetic Engineering and Biotechnology News
dlvr.it
May 22, 2025 at 2:00 AM
Thoughts on this? >> A Suite of Enhancer AAVs Support Targeted Gene Therapy for
Brain Disease - Genetic Engineering and Biotechnology News >> Comment below! #strategy #competitiveintelligence #marketing #healthcare #pharmaceutical #biotech #pharma #competitivemarketing
Brain Disease - Genetic Engineering and Biotechnology News >> Comment below! #strategy #competitiveintelligence #marketing #healthcare #pharmaceutical #biotech #pharma #competitivemarketing
A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types @cellcellpress.bsky.social
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
May 21, 2025 at 8:29 PM
A suite of enhancer AAVs and transgenic mouse lines for genetic access to cortical cell types @cellcellpress.bsky.social
www.cell.com/cell/fulltex...
www.cell.com/cell/fulltex...
From the company website. Quote below.
Designed for rapid assembly at low cost, the Barracuda family of AAVs only requires ten or fewer tools to assemble, ensuring that both new and existing production lines can be rapidly spun up to produce AAVs at hyper-scale.
Designed for rapid assembly at low cost, the Barracuda family of AAVs only requires ten or fewer tools to assemble, ensuring that both new and existing production lines can be rapidly spun up to produce AAVs at hyper-scale.
October 12, 2025 at 5:45 PM
From the company website. Quote below.
Designed for rapid assembly at low cost, the Barracuda family of AAVs only requires ten or fewer tools to assemble, ensuring that both new and existing production lines can be rapidly spun up to produce AAVs at hyper-scale.
Designed for rapid assembly at low cost, the Barracuda family of AAVs only requires ten or fewer tools to assemble, ensuring that both new and existing production lines can be rapidly spun up to produce AAVs at hyper-scale.
For sure. I'm kind of resigned to the notion he will (if he re-signs) end up somewhere between Matthews' and Nylander's AAVs. I just want 8 years, the Leafs are getting killed partially by having to re-up their stars faster than others (and for higher AAVs)
November 19, 2024 at 2:16 AM
For sure. I'm kind of resigned to the notion he will (if he re-signs) end up somewhere between Matthews' and Nylander's AAVs. I just want 8 years, the Leafs are getting killed partially by having to re-up their stars faster than others (and for higher AAVs)