Keith Zientek
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zientek.bsky.social
Keith Zientek
@zientek.bsky.social
Core scientist
At some point all the AI bots will just talk to each other via Office and Teams and we can really get some work done while they are distracted.
September 3, 2025 at 9:37 PM
It really forces you to look at the abstract qualities of your skills and experience. From the exciting stuff like troubleshooting and instrument operation to the common skills like excel spreadsheets, etc.
August 29, 2025 at 4:34 PM
I went through this 6 years ago. Realistically there were only two places in my city that were a match for my skillset. I worked for one and they were shut down. While I was trying to get a job at #2, I went on a bunch of interviews and applied for jobs that were overlapping with my background.
August 29, 2025 at 4:31 PM
I'm not familiar with the plus but I have used the QE for a PRM method with an inclusion list. It's a weirdly worded setting. You can either have it cycle through the inclusion list or have it trigger DDA scans if the masses on the list aren't detected.
August 27, 2025 at 10:32 PM
I guess you could get a job at a company doing something similar but you would have to add one bajillion zoom calls that could have been emails to your weekly schedule.
July 14, 2025 at 6:58 PM
Not proteomics but I am reminded of the petroleum work done by Alan Marshall’s lab
June 20, 2025 at 12:59 PM
Now I want to know about variants 1 through 6.
June 19, 2025 at 12:20 AM
I hope it can answer advanced PhD level queries like where is the cheapest cafeteria and where is the nice bathroom.
June 2, 2025 at 9:56 PM
Use the free time to run more Western blots as required by reviewer 3 for every paper.
May 15, 2025 at 1:09 PM
Quick invest in an autosampler vial company.
April 27, 2025 at 11:34 PM
Why limit yourself to just the hot dogs when you can work in the cafe and operate the pizza robot?

youtu.be/c-E2Cz59_5A?...
🍕 Pizza making at Costco Too Quick to eat Pizza robot unmanned pizza 🍕 😋
YouTube video by Hariax Thoria
youtu.be
March 20, 2025 at 8:31 PM
Imagine the savings if you had an employee discount on top of the normal deals. And easy access to the hot dogs!
March 20, 2025 at 7:56 PM
Was hired to run a very small bioanalytical core and learned on the job with help from biochemistry post docs and a neighboring proteomics mass spec core facility.
March 20, 2025 at 1:22 PM
When identifying candidates form untargeted quan for further study/grants do you ever go back and look at the raw data to see things like # of peptides per protein and # of points per peak?
March 18, 2025 at 10:08 PM
Is that >2 peptides per protein?
March 18, 2025 at 5:01 PM
I’d love to see people consider metrics other than # of proteins detected.
March 6, 2025 at 3:08 PM
I bet you could get a bunch of Zip Drive discs or 3.5 floppys at a deep discount.
March 5, 2025 at 4:22 AM
Nevertheless
Science
For
Work
February 12, 2025 at 6:26 PM
I wonder how many Terrabytes or Petabytes of Astral raw files would be generated in that study? Maybe even get to Exabytes?
January 10, 2025 at 6:03 PM
Unless you are monitoring a single cell over time, shouldn't the population be similar? You have a cells going away but also being created so a snapshot should include all of the various timepoints in theory?
January 10, 2025 at 5:41 PM
We are just starting with DIA so we are trying to determine best practices for QC. That was the reason behind my question.

Are your protein ID numbers based on 1 peptide per protein or another value >1?
January 7, 2025 at 5:54 PM
What do you mean by data completeness? Are you looking at detected PSMs versus total scans?
January 7, 2025 at 5:02 PM
What other metrics do you use to assess quality other than number of detected proteins?
January 7, 2025 at 3:16 PM