Research topic: Genomics | Transcriptomics | Long-read sequencing | Single-cell sequencing.
The entire dataset is openly available for anyone developing tools or benchmarking long-read technologies.
The entire dataset is openly available for anyone developing tools or benchmarking long-read technologies.
• Bulk, single-cell & single-nucleus RNA-seq from 8 lung-cancer cell lines spanning 3 cancer types for realistic DE analysis
• Three long-read protocols (ONT PCR-cDNA, ONT direct RNA, PacBio Kinnex) and Illumina short-read sequencing
• Synthetic spike-in controls for ground truth
• Bulk, single-cell & single-nucleus RNA-seq from 8 lung-cancer cell lines spanning 3 cancer types for realistic DE analysis
• Three long-read protocols (ONT PCR-cDNA, ONT direct RNA, PacBio Kinnex) and Illumina short-read sequencing
• Synthetic spike-in controls for ground truth
LongBench enables rigorous cross-platform comparisons of gene & isoform quantification, differential expression, alternative splicing, and allele-specific expression—helping the community evaluate and improve long-read methods.
LongBench enables rigorous cross-platform comparisons of gene & isoform quantification, differential expression, alternative splicing, and allele-specific expression—helping the community evaluate and improve long-read methods.