O. Kerscher
@yeastlife.bsky.social
SUMO in stress tolerance and protein quality control | lab life | yeast genetics | undergraduate research | backyard nature | opinions my own
Once resuspended the pi is 50x. Basically we spin down 150-200 OD of cells and resuspend in 1ml lysis buffer of choice with 1x Pi. The protocol is here: PMID: 24300101. I’d be glad to share a pdf if you don’t have access .
November 25, 2025 at 1:40 PM
Once resuspended the pi is 50x. Basically we spin down 150-200 OD of cells and resuspend in 1ml lysis buffer of choice with 1x Pi. The protocol is here: PMID: 24300101. I’d be glad to share a pdf if you don’t have access .
Promega protease inhibitor cocktail G6521 - good luck!
November 24, 2025 at 6:43 PM
Promega protease inhibitor cocktail G6521 - good luck!