I guess that means Alex blocked me rather than try answering

But the passaged BANAL-236 virus did not have an FCS, which is what turned an intestinal virus into a respiratory one. To do a proper investigation whether @solidevidence.bsky.social’s SARS2 mutational pattern would arise via passaging (e.g. in HAE cells), an FCS should be added first.

How do you reconcile this absence of a strong selective pressure signal with your hypothesis of the SARS2 progenitor circulating in an intermediate host for a few years before spilling over to humans?

Kristian, what dN/dS signatures of culture adaptation would you expect passaging in HAE cells to produce?