Kang Wu
@wukang.bsky.social
Maybe be passionate about bioinformatics, immunotherapy, and diagnosis, haha! Website https://iamhealthy.github.io/
"... through experimental evolution over the course of 30 days, were able to vastly expand the host ranges of these phages to kill a broader range of clinical K. pneumoniae isolates including MDR (multi-drug resistant) and XDR (extensively-drug resistant) isolates."
www.nature.com/articles/s41...
www.nature.com/articles/s41...
November 26, 2025 at 7:35 AM
"... through experimental evolution over the course of 30 days, were able to vastly expand the host ranges of these phages to kill a broader range of clinical K. pneumoniae isolates including MDR (multi-drug resistant) and XDR (extensively-drug resistant) isolates."
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Small vesicles are everywhere, intracellular and extracellular.
Vesicles can be a mix from the host and (infected) pathogen.
Vesicles are a way for distant communication, besides via single molecules.
Communication might result in some outcomes (good or bad).
www.biorxiv.org/content/10.1...
Vesicles can be a mix from the host and (infected) pathogen.
Vesicles are a way for distant communication, besides via single molecules.
Communication might result in some outcomes (good or bad).
www.biorxiv.org/content/10.1...
November 21, 2025 at 2:53 AM
Small vesicles are everywhere, intracellular and extracellular.
Vesicles can be a mix from the host and (infected) pathogen.
Vesicles are a way for distant communication, besides via single molecules.
Communication might result in some outcomes (good or bad).
www.biorxiv.org/content/10.1...
Vesicles can be a mix from the host and (infected) pathogen.
Vesicles are a way for distant communication, besides via single molecules.
Communication might result in some outcomes (good or bad).
www.biorxiv.org/content/10.1...
November 10, 2025 at 1:00 AM
A good paper for knowing:
What mp-tNGS (multiplex-PCR targeted NGS) is.
What hc-tNGS (hybrid-capture targeted NGS) is.
www.thelancet.com/journals/ebi...
What mp-tNGS (multiplex-PCR targeted NGS) is.
What hc-tNGS (hybrid-capture targeted NGS) is.
www.thelancet.com/journals/ebi...
September 4, 2025 at 2:53 AM
A good paper for knowing:
What mp-tNGS (multiplex-PCR targeted NGS) is.
What hc-tNGS (hybrid-capture targeted NGS) is.
www.thelancet.com/journals/ebi...
What mp-tNGS (multiplex-PCR targeted NGS) is.
What hc-tNGS (hybrid-capture targeted NGS) is.
www.thelancet.com/journals/ebi...
Common pathogens infecting the urinary tract:
Escherichia coli
Proteus mirabilis
Streptococcus agalactiae
Klebsiella pneumoniae
Pseudomonas aeruginosa
Staphylococcus aureus
Enterococcus faecalis
Staphylococcus saprophyticus
Acinetobacter baumannii
Candida albicans
www.nature.com/articles/s41...
Escherichia coli
Proteus mirabilis
Streptococcus agalactiae
Klebsiella pneumoniae
Pseudomonas aeruginosa
Staphylococcus aureus
Enterococcus faecalis
Staphylococcus saprophyticus
Acinetobacter baumannii
Candida albicans
www.nature.com/articles/s41...
September 3, 2025 at 6:30 AM
Common pathogens infecting the urinary tract:
Escherichia coli
Proteus mirabilis
Streptococcus agalactiae
Klebsiella pneumoniae
Pseudomonas aeruginosa
Staphylococcus aureus
Enterococcus faecalis
Staphylococcus saprophyticus
Acinetobacter baumannii
Candida albicans
www.nature.com/articles/s41...
Escherichia coli
Proteus mirabilis
Streptococcus agalactiae
Klebsiella pneumoniae
Pseudomonas aeruginosa
Staphylococcus aureus
Enterococcus faecalis
Staphylococcus saprophyticus
Acinetobacter baumannii
Candida albicans
www.nature.com/articles/s41...
Below is the principle (copied and edited from the original paper):
Given that RNA in FFPE samples is usually degraded and often highly fragmented due to the cross-linking effect of formaldehyde,
pubmed.ncbi.nlm.nih.gov/40882628/
Given that RNA in FFPE samples is usually degraded and often highly fragmented due to the cross-linking effect of formaldehyde,
pubmed.ncbi.nlm.nih.gov/40882628/
August 31, 2025 at 1:19 AM
Below is the principle (copied and edited from the original paper):
Given that RNA in FFPE samples is usually degraded and often highly fragmented due to the cross-linking effect of formaldehyde,
pubmed.ncbi.nlm.nih.gov/40882628/
Given that RNA in FFPE samples is usually degraded and often highly fragmented due to the cross-linking effect of formaldehyde,
pubmed.ncbi.nlm.nih.gov/40882628/
From this image, at least we could know the general appearances of several important bacterial pathogens. 😁
www.mdpi.com/2076-393X/13...
www.mdpi.com/2076-393X/13...
August 24, 2025 at 9:16 AM
From this image, at least we could know the general appearances of several important bacterial pathogens. 😁
www.mdpi.com/2076-393X/13...
www.mdpi.com/2076-393X/13...
(i) genetic variation, (ii) individual-scale generation of additional genetic diversity solely in lymphocytes, and (iii) **collision** of these processes with a changing environment through life, triggering plastic responses that can have lasting effects.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
August 14, 2025 at 6:51 AM
(i) genetic variation, (ii) individual-scale generation of additional genetic diversity solely in lymphocytes, and (iii) **collision** of these processes with a changing environment through life, triggering plastic responses that can have lasting effects.
www.science.org/doi/10.1126/...
www.science.org/doi/10.1126/...
Mtb infecting humans exclusively and Mtb infecting both humans and other species are different phylogenetically.
www.medrxiv.org/content/10.1...
#tuberculosis
www.medrxiv.org/content/10.1...
#tuberculosis
August 11, 2025 at 5:00 AM
Mtb infecting humans exclusively and Mtb infecting both humans and other species are different phylogenetically.
www.medrxiv.org/content/10.1...
#tuberculosis
www.medrxiv.org/content/10.1...
#tuberculosis
- "We demonstrate enhanced T cell responses in the lung following BCG + ID93-GLA-SE compared to BCG or ID93-GLA-SE alone, with **notable sex differences in humoral immunity**"
- "a high-dimensional flow cytometry (12 or more
colors)"
#tuberculosis #immunity #vaccine
www.nature.com/articles/s41...
- "a high-dimensional flow cytometry (12 or more
colors)"
#tuberculosis #immunity #vaccine
www.nature.com/articles/s41...
August 11, 2025 at 3:22 AM
- "We demonstrate enhanced T cell responses in the lung following BCG + ID93-GLA-SE compared to BCG or ID93-GLA-SE alone, with **notable sex differences in humoral immunity**"
- "a high-dimensional flow cytometry (12 or more
colors)"
#tuberculosis #immunity #vaccine
www.nature.com/articles/s41...
- "a high-dimensional flow cytometry (12 or more
colors)"
#tuberculosis #immunity #vaccine
www.nature.com/articles/s41...
Images of two different viruses, one for infecting some mycobacteria and the other one for infecting marine dinoflagellates.
The marine one has the longest tail (~ 2.3 um) as far as the author knows.
www.sciencedirect.com/science/arti...
www.biorxiv.org/content/10.1...
The marine one has the longest tail (~ 2.3 um) as far as the author knows.
www.sciencedirect.com/science/arti...
www.biorxiv.org/content/10.1...
August 2, 2025 at 8:35 AM
Images of two different viruses, one for infecting some mycobacteria and the other one for infecting marine dinoflagellates.
The marine one has the longest tail (~ 2.3 um) as far as the author knows.
www.sciencedirect.com/science/arti...
www.biorxiv.org/content/10.1...
The marine one has the longest tail (~ 2.3 um) as far as the author knows.
www.sciencedirect.com/science/arti...
www.biorxiv.org/content/10.1...
Creative and beautiful scientific artwork
bead + antibody + rna + rbp
should took lots of "sweat"~
www.sciencedirect.com/science/arti...
bead + antibody + rna + rbp
should took lots of "sweat"~
www.sciencedirect.com/science/arti...
July 27, 2025 at 5:46 AM
Creative and beautiful scientific artwork
bead + antibody + rna + rbp
should took lots of "sweat"~
www.sciencedirect.com/science/arti...
bead + antibody + rna + rbp
should took lots of "sweat"~
www.sciencedirect.com/science/arti...
So many new 2A peptides, of which several are stronger than the often-used P2A.
How about prefixing GSG to those new and stronger ones?
Systematic identification and characterization of eukaryotic and viral 2A peptide-bond-skipping sequences: Cell Reports www.cell.com/cell-reports...
How about prefixing GSG to those new and stronger ones?
Systematic identification and characterization of eukaryotic and viral 2A peptide-bond-skipping sequences: Cell Reports www.cell.com/cell-reports...
July 25, 2025 at 1:28 PM
So many new 2A peptides, of which several are stronger than the often-used P2A.
How about prefixing GSG to those new and stronger ones?
Systematic identification and characterization of eukaryotic and viral 2A peptide-bond-skipping sequences: Cell Reports www.cell.com/cell-reports...
How about prefixing GSG to those new and stronger ones?
Systematic identification and characterization of eukaryotic and viral 2A peptide-bond-skipping sequences: Cell Reports www.cell.com/cell-reports...
An innovation of vaccination route: targeting the gingival sulcus via antigen-coated floss.
www.nature.com/articles/s41...
www.science.org/content/arti...
www.nature.com/articles/s41...
www.science.org/content/arti...
July 23, 2025 at 1:06 AM
An innovation of vaccination route: targeting the gingival sulcus via antigen-coated floss.
www.nature.com/articles/s41...
www.science.org/content/arti...
www.nature.com/articles/s41...
www.science.org/content/arti...
PTB or HIV donors have adverse drug reactions (46.58% and 33.33% respectively).
How about vaccine candidates containing ESAT-6 and/or CFP10?
ESAT6-CPF10 Skin Test in A Large Population: A Phase IV Clinical Trial url: academic.oup.com/jid/article-...
How about vaccine candidates containing ESAT-6 and/or CFP10?
ESAT6-CPF10 Skin Test in A Large Population: A Phase IV Clinical Trial url: academic.oup.com/jid/article-...
June 29, 2025 at 9:59 AM
PTB or HIV donors have adverse drug reactions (46.58% and 33.33% respectively).
How about vaccine candidates containing ESAT-6 and/or CFP10?
ESAT6-CPF10 Skin Test in A Large Population: A Phase IV Clinical Trial url: academic.oup.com/jid/article-...
How about vaccine candidates containing ESAT-6 and/or CFP10?
ESAT6-CPF10 Skin Test in A Large Population: A Phase IV Clinical Trial url: academic.oup.com/jid/article-...
Small proteins should exist in all organisms (e.g., humans, mice, M. tuberculosis, and even viruses).
Perhaps they participate in many aspects of processes usually performed by large proteins.
www.cell.com/trends/micro...
Perhaps they participate in many aspects of processes usually performed by large proteins.
www.cell.com/trends/micro...
June 4, 2025 at 1:39 AM
Small proteins should exist in all organisms (e.g., humans, mice, M. tuberculosis, and even viruses).
Perhaps they participate in many aspects of processes usually performed by large proteins.
www.cell.com/trends/micro...
Perhaps they participate in many aspects of processes usually performed by large proteins.
www.cell.com/trends/micro...
Dual (both zebrafish and M. marinum) RNA-seq.
After the enrichment of M. marinum mRNA, the percentages could be increased to 0.5% - 1.0% (one outlier of > 2.0%), from around 0.1%.
10 times of enrichment.
So the sequencing depth should still be enlarged.
www.biorxiv.org/content/10.1...
After the enrichment of M. marinum mRNA, the percentages could be increased to 0.5% - 1.0% (one outlier of > 2.0%), from around 0.1%.
10 times of enrichment.
So the sequencing depth should still be enlarged.
www.biorxiv.org/content/10.1...
May 21, 2025 at 1:51 AM
Dual (both zebrafish and M. marinum) RNA-seq.
After the enrichment of M. marinum mRNA, the percentages could be increased to 0.5% - 1.0% (one outlier of > 2.0%), from around 0.1%.
10 times of enrichment.
So the sequencing depth should still be enlarged.
www.biorxiv.org/content/10.1...
After the enrichment of M. marinum mRNA, the percentages could be increased to 0.5% - 1.0% (one outlier of > 2.0%), from around 0.1%.
10 times of enrichment.
So the sequencing depth should still be enlarged.
www.biorxiv.org/content/10.1...
Perhaps use PubMed and Europe PMC in parallel later~~
May 16, 2025 at 1:13 AM
Perhaps use PubMed and Europe PMC in parallel later~~
At least I know there are (plenty of) commercial moves pushing NAD+ supplements, telomere length measuring, immune monitoring via FACS, and injecting MSCs/NK for every health condition here in CN. ~~
May 15, 2025 at 2:56 AM
At least I know there are (plenty of) commercial moves pushing NAD+ supplements, telomere length measuring, immune monitoring via FACS, and injecting MSCs/NK for every health condition here in CN. ~~
"BCG revaccination in QFT–test negative, HIV-negative adolescents did not provide protection from sustained M. tuberculosis infection."
Waiting to see the result of preventing the TB disease.
www.nejm.org/doi/10.1056/...
Waiting to see the result of preventing the TB disease.
www.nejm.org/doi/10.1056/...
May 9, 2025 at 3:41 AM
"BCG revaccination in QFT–test negative, HIV-negative adolescents did not provide protection from sustained M. tuberculosis infection."
Waiting to see the result of preventing the TB disease.
www.nejm.org/doi/10.1056/...
Waiting to see the result of preventing the TB disease.
www.nejm.org/doi/10.1056/...
"Natural" killer cells in the mouse
I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype
Eur. J. Immunol. 1975. 5: 112-117
I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype
Eur. J. Immunol. 1975. 5: 112-117
April 22, 2025 at 6:10 AM
"Natural" killer cells in the mouse
I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype
Eur. J. Immunol. 1975. 5: 112-117
I. Cytotoxic cells with specificity for mouse Moloney leukemia cells. Specificity and distribution according to genotype
Eur. J. Immunol. 1975. 5: 112-117
Strong translation initiation codons vs. weak translation initiation codons.
New to me, even though the paper was published in 2017.
academic.oup.com/nar/article/...
New to me, even though the paper was published in 2017.
academic.oup.com/nar/article/...
April 21, 2025 at 1:01 AM
Strong translation initiation codons vs. weak translation initiation codons.
New to me, even though the paper was published in 2017.
academic.oup.com/nar/article/...
New to me, even though the paper was published in 2017.
academic.oup.com/nar/article/...
"lab-in-tube"
RPA + Cas12a
Rapid tuberculosis diagnosis from respiratory or blood samples by a low cost, portable lab-in-tube assay | Science Translational Medicine www.science.org/doi/10.1126/...
RPA + Cas12a
Rapid tuberculosis diagnosis from respiratory or blood samples by a low cost, portable lab-in-tube assay | Science Translational Medicine www.science.org/doi/10.1126/...
April 11, 2025 at 3:05 PM
"lab-in-tube"
RPA + Cas12a
Rapid tuberculosis diagnosis from respiratory or blood samples by a low cost, portable lab-in-tube assay | Science Translational Medicine www.science.org/doi/10.1126/...
RPA + Cas12a
Rapid tuberculosis diagnosis from respiratory or blood samples by a low cost, portable lab-in-tube assay | Science Translational Medicine www.science.org/doi/10.1126/...
This small all-in-one (from sample to results) PCR device/platform is fantastic.
A PhD student sitting next to me is also designing similar equipment de novo (his main PhD work) and is interested in this device.
This device should have some impact on him.
Haha.
www.visbymedical.com/publication/...
A PhD student sitting next to me is also designing similar equipment de novo (his main PhD work) and is interested in this device.
This device should have some impact on him.
Haha.
www.visbymedical.com/publication/...
April 2, 2025 at 1:44 AM
This small all-in-one (from sample to results) PCR device/platform is fantastic.
A PhD student sitting next to me is also designing similar equipment de novo (his main PhD work) and is interested in this device.
This device should have some impact on him.
Haha.
www.visbymedical.com/publication/...
A PhD student sitting next to me is also designing similar equipment de novo (his main PhD work) and is interested in this device.
This device should have some impact on him.
Haha.
www.visbymedical.com/publication/...
"SABER could convert 30% of circulating CD8+ T cells into antigen-specific cells, a proportion that rarely exceeded
5% by other adjuvants used in this study for the same antigens."
PS. I read for the 1st time that THP-1 cells were induced to DC-like cells. :-)
rdcu.be/efpvH
5% by other adjuvants used in this study for the same antigens."
PS. I read for the 1st time that THP-1 cells were induced to DC-like cells. :-)
rdcu.be/efpvH
March 28, 2025 at 1:07 AM
"SABER could convert 30% of circulating CD8+ T cells into antigen-specific cells, a proportion that rarely exceeded
5% by other adjuvants used in this study for the same antigens."
PS. I read for the 1st time that THP-1 cells were induced to DC-like cells. :-)
rdcu.be/efpvH
5% by other adjuvants used in this study for the same antigens."
PS. I read for the 1st time that THP-1 cells were induced to DC-like cells. :-)
rdcu.be/efpvH