Methylobacterium strains are common on human skin, but their genomic information is limited (1, 2). Here, we report the genome sequence of a Methylobacterium strain isolated from human skin. A forehead skin swab from a female college student was streaked on minimal salts basal medium agar (3) with 0.5% Triton X-100 as a carbon and energy source. After 1 week of aerobic incubation at 28°C, one isolate, OT2, was purified on Nutrient agar (KisanBio Co) and incubated for 4 days at 28°C. The strain was deposited in the Korean Collection for Type Cultures as KCTC 82560 and Japan Collection of Microorganisms as JCM 34944 (NCBI taxonomy ID 2813779). Ethical approval for subject sampling was granted by the institutional review board of Changwon National University. Sanger sequencing of the 16S rRNA gene using primers 27F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R (5′-GGTTACCTTGTTACGACTT-3′), along with BLASTN analysis (4), identified OT2 as a member of the genus Methylobacterium. It showed the highest similarity to M. oryzae CBMB20T (99.63%) and M. fujisawaense strain DSM 5686T (99.63%).

Abstract. Our understanding of translation underpins our capacity to engineer living systems. The canonical start codon (AUG) and a few near-cognates (GUG,