Chris Risher
@wc82rish.bsky.social
Husband, father, neuroscientist, glia enthusiast, music & film geek, Taekwondo 1st Gup. Half Caucasian/half Filipino. He/him/his. Views are my own. chrisrisherlab.com
For my cryostat folks: we're trying to section some PFA-fixed, TFM-embedded brains at 10 um thickness, and were having a rough time of it. I've spent the majority of my career cutting 20 um or thicker, so this isn't my forte. Any do's, don'ts, or other tips you got?
June 17, 2025 at 6:32 PM
For my cryostat folks: we're trying to section some PFA-fixed, TFM-embedded brains at 10 um thickness, and were having a rough time of it. I've spent the majority of my career cutting 20 um or thicker, so this isn't my forte. Any do's, don'ts, or other tips you got?