Finally, this work gives the most time-resolved overview of transcription during the life cycle of Bdello. There is much data to delve into, but we already show that there are a lot of specific patterns going on between attack and exit. 6/n

The attack phase remains elusive. We tried disturbing the nucleoid with transcription and translation-inhibiting antibiotics, but even adding more than 100x MIC didn't change the shape. It didn't even stop them swimming around happily. 5/n

Attack phase chromosome? Highly compacted. But when replication starts, the Bdello chromosome adapts an arm/arm interconnected conformation, starting at the origin of replication. You can basically see SMC/ParB at work! 4/n

This made us wonder: What happens with the chromosome during the bdello life cycle? Together with Fares Osam, postdoc @rkoszul.bsky.social we set out to map the chromosome conformation during the full cell cycle using Hi-C combined with RNA-seq. This are the key take outs: 3/n

stickers niet, maar ik heb lego op m'n laptop 😎- dat is wel een nachtmerrie als je het brandschoon wil houden

Found back my chrochet-pneumococcus while moving houses 🦠🧶

I got a 3D printer for the lab and it's so much fun! First prints: magnetic fridge holders for the multipipettes & 96-well holders for in our old infors shaker incubator. 🦠🧫
designs adapted from www.thingiverse.com/thing:6430834 and www.thingiverse.com/thing:5416767

Are you interested in how the bacterial predator Bdellovibrio kills & eats so many bugs so efficiently and do you want to use cool microscopy & systems methods to find out? Come work with me in the best city of the Netherlands! Fully funded PhD (4 years) -> www.rug.nl/about-ug/wor... 🦠🧫

Second week as a #newpi and i am at home with a fever! The only thing progressing faster than anticipated this week is this sock 🤷🧶 🦠

This month it is 21 years ago that my dad passed away. He was very good at dad jokes, walking on his hands, climbing trees and doing silly walks. This is what I wrote about him in my thesis acknowledgments:

Quite challenging but massively interesting & useful to follow the DL4MIA course @florianjug.bsky.social! Including precious life lessons as 'cultivate your laziness' - don't process data when it's not necessary