Excited to be speaking at #ASHG2025 this week in Boston!

All data are available in a user-friendly interactive Shiny app that you can use to search your favorite genes across conditions (link in the Methods section).

We also investigated splicing dynamics during activation, identifying stimulus-dependent alternative splicing events that affect gene function.

We focused on the SLE-risk variant at the TNFSF4 locus, which lies in a stimulus-dependent open chromatin region, highlighting how studying B cell activation can inform variant function.

We used ATAC-seq to understand chromatin remodeling during activation. We identified key transcription factors and found that stimulus-induced open chromatin regions are enriched for immune-mediated disease heritability.

Using single-cell RNA-seq + CITE-seq, we showed how B cells differentiate under different stimuli, revealing stimulus-dependent fates.

We used bulk RNA-seq to profile B cell transcriptional responses to different stimuli. We uncovered stimulus-dependent gene programs with distinct dynamics and functions.

I’m excited to share my latest work with Marcella Franco, @mariagtac.bsky.social, and an amazing team. We used multi-omics to dissect key pathways of B cell activation, exploring gene programs, splicing, cell fates, and disease heritability in activated B cells.

www.medrxiv.org/content/10.1...

In Monte Carlo, Monaco, for the Keystone Symposium on B cells! On Wednesday, I will talk about our work on B cell activation.

Estimates of the constraint metric s_het from @jkpritch.bsky.social work (doi.org/10.1038/s415...) for a curated list of lupus susceptibility genes. IKZF1 is the SLE risk gene with the most extreme selection on LOF mutations.

Glad to work with this group

It was a very inspiring talk!

Sad to miss #ASHG2023 but I will advertise a talk by Nicolaj Hackert and Dany Salinas!