@vicente-trelles.bsky.social
DPhil Student at Steel Lab - University of Oxford. High throughput single cell microscopy applied to screening and directed evolution.
Reposted
Some exciting results to share! The MagLOV and related proteins from @andrewgyork.bsky.social and @mariaingaramo.bsky.social are quantum. We measured 4% optically detected magnetic resonance contrast in living bacterial cells at room temperature. www.biorxiv.org/content/10.1...
![A. Structure of AsLOV2 [PDB 2V1A] with mutations resutling in MagLOV~2 highlighted. For the first time, quantum sensing in living cells using a fluorescent protein-based sensor can be performed by driving spin transitions using radio-frequency (RF) fields in the presence of a static magnetic field. B. Simplified photocycle diagram in the case of a large external magnetic field.
C. Microscope image cropped to a single cell expressing MagLOV 2. Scalebar is 1 um.
D. For MFE measurements the magnetic field was modulated between 0 mT and 10 mT, here with a period 10 seconds. The intensity over time is integrated over pixels covering the single cell, with an exponential decay (due to photobleaching) removed.
E. A single cell expressing MagLOV 2 displaying an ODMR signal 4% contrast.](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:tludcv74nxumusfwzkmfcl5q/bafkreigmpmmy3xz6alxsxwwmsw4dxn7lkebjl4lqtcwvpiljdiubztlmqa@jpeg)
November 28, 2024 at 3:13 PM
Some exciting results to share! The MagLOV and related proteins from @andrewgyork.bsky.social and @mariaingaramo.bsky.social are quantum. We measured 4% optically detected magnetic resonance contrast in living bacterial cells at room temperature. www.biorxiv.org/content/10.1...