Aurelia
@vesiclesmasher.bsky.social
Actually, these days I mostly encourage others to smash vesicles. Associate professor in the Department of Physics at Lehigh University.
Not bad looking, Lehigh
November 5, 2025 at 7:13 PM
Not bad looking, Lehigh
In addition to fluorescence microscopy, they observed dowstream drift of T-cadherin molecules on Xenopus cells using single-molecule tracking! Flow-mediated protein redistribution might explain how cells respond to a wide variety of biological flows.
August 30, 2025 at 3:17 AM
In addition to fluorescence microscopy, they observed dowstream drift of T-cadherin molecules on Xenopus cells using single-molecule tracking! Flow-mediated protein redistribution might explain how cells respond to a wide variety of biological flows.
A smaller protein, GPI-GFP, doesn’t move much under 2 Pascale of shear stress. But we can convert it from immobile to mobile under 2 Pa by allowing a GFP antibody to bind, increasing its size and the hydrodynamic force applied to it (Sreeja measured the force).
August 30, 2025 at 3:17 AM
A smaller protein, GPI-GFP, doesn’t move much under 2 Pascale of shear stress. But we can convert it from immobile to mobile under 2 Pa by allowing a GFP antibody to bind, increasing its size and the hydrodynamic force applied to it (Sreeja measured the force).
I am so excited for everyone to see this! Dr. Sreeja Sasidharan, legendary postdoc, recently showed that flow moves proteins sideways through a glass-supported lipid bilayer. We wanted to see if this could happen on the surface of living cells! AND IT CAN
August 30, 2025 at 3:17 AM
I am so excited for everyone to see this! Dr. Sreeja Sasidharan, legendary postdoc, recently showed that flow moves proteins sideways through a glass-supported lipid bilayer. We wanted to see if this could happen on the surface of living cells! AND IT CAN
I haven't quantified, but DPPE-cap-Biotin from Avanti seems to go obligingly into the Lo phase (at least on splats). We tried biotinylated cholesterol once, but couldn't get any avidin to stick to it.
January 9, 2025 at 2:35 AM
I haven't quantified, but DPPE-cap-Biotin from Avanti seems to go obligingly into the Lo phase (at least on splats). We tried biotinylated cholesterol once, but couldn't get any avidin to stick to it.
Proteins with similar molecular weights, but different shapes, move at different speeds. It only takes a femtonewton or so to scoot a protein along in the membrane! You can also see a simulation by the Im lab showing how a streptavidin monomer sits on its biotinylated lipid.
September 16, 2024 at 11:03 PM
Proteins with similar molecular weights, but different shapes, move at different speeds. It only takes a femtonewton or so to scoot a protein along in the membrane! You can also see a simulation by the Im lab showing how a streptavidin monomer sits on its biotinylated lipid.
Hurray! Today Sreeja’s beautiful experiments are finally published: authors.elsevier.com/a/1jm9E1SPT7...
Cell membranes contain a lot of proteins. How big are they, and what shape? How far do they stick up? Sreeja measures protein size and shape using flow:
Cell membranes contain a lot of proteins. How big are they, and what shape? How far do they stick up? Sreeja measures protein size and shape using flow:
September 16, 2024 at 11:02 PM
Hurray! Today Sreeja’s beautiful experiments are finally published: authors.elsevier.com/a/1jm9E1SPT7...
Cell membranes contain a lot of proteins. How big are they, and what shape? How far do they stick up? Sreeja measures protein size and shape using flow:
Cell membranes contain a lot of proteins. How big are they, and what shape? How far do they stick up? Sreeja measures protein size and shape using flow: