Vadim Demichev
vadim-demichev.bsky.social
Vadim Demichev
@vadim-demichev.bsky.social
Group Leader at Charité – Universitätsmedizin Berlin. Proteomics technologies and applications, DIA-NN author. https://aptila.bio
Most likely only one isoform will be reported, matching the better of the spectra
October 25, 2025 at 7:09 PM
Very unlikely to get identified correctly
October 25, 2025 at 5:07 PM
Yes, it will. Although without MS/MS evidence the reported localisation confidence will be low and the overall numbers of correctly assigned isomers are likely to be low. Those IDs with MS/MS evidence should be fine though.
October 25, 2025 at 4:43 PM
I think this may change with MBR on samples from different patients, in particular for neat.
October 7, 2025 at 5:04 PM
That said, it's definitely a good idea for us to provide a tutorial and example code on this. Will add to todo list.
October 7, 2025 at 2:02 PM
Currently not saved automatically, main issue: a peptide can match multiple positions even within the sequence of a single protein. However, this can be done with a script, matching to protein sequence in protein_description.tsv output file. AI will easily write such a script in 5 min :)
October 7, 2025 at 2:01 PM
Probably not, we are completely overwhelmed with paper writing at the moment, so only selected stuff eventually will get published. Short application notes are more likely though:)
September 27, 2025 at 8:02 AM