Please consider La Châtaigne in Lafayette. I came back from two weeks living in the Marais this summer and having the real thing daily. The croissant beurre d’Isigny at La C is as close to the original as one can imagine. Get there early - they sell out fast. They open at 8. 🇫🇷

I will switch out of "dripping with scathing sarcasm" mode and cite a lovely paper by David, Shengdar, Mitch, et al - one of many such papers - where this technically trivial experiment is done.
www.nature.com/articles/s41...

Around back then I became an ace at dispensing with it 500 ul PB into 20 qiagen columns without splashing chaotrope all over the bench.

+1
My students have a digital one and my reaction is - why?

This is v helpful.
I'd love to know how many genes are like Car2.
Five percent? Ten percent?
Because looping is everywhere, and so-called TADs as well, but if 5% of the genes actually depend on looping for their control - well, this means the vast majority of looping is gene-control irrelevant.

I missed this paper - this is an important finding - will read. Thank you!

At Vijay's suggestion I looked at your paper (Hansen et al).
So would you say Cas2 is the poster child for cohesin-mediated loop essentiality in gene control? B/c clearly for Sox2 it's not true. What other genes like Car2 are this well-characterized wrt this dependency?

Stefan, I agree with you completely.
Is there evidence that eliminating cohesin-mediated looping affects HBA expression in adult human erythropoiesis?
(apologies if I missed it)
I am fine with local looping that's cohesin-independent as instructive to transcription.

Precisely.

I mean, you kill looping and transcription goes - whatevs.
This is a major epistemological challenge.

You realize this is a Sangamo paper by my colleagues when I was there? 🤓
What can be showed to happen does not mean happens naturally. 🤓