@unitsaq.bsky.social
Interested in LC-MS -omics (proteomics, metabolomics, lipidomics, exposomics).
Distilled rule of thumb for optimal Protein:Trypsin ratio vs Total Protein concentration in bottom-up #proteomics:
Optimal protein:trypsin ≈ 0.1 * protein concentration (ng/uL)
Aligns well with standard guidelines e.g. 50:1 at ~500 ng/uL
Data from Table 5 in www.sciencedirect.com/science/arti...
Optimal protein:trypsin ≈ 0.1 * protein concentration (ng/uL)
Aligns well with standard guidelines e.g. 50:1 at ~500 ng/uL
Data from Table 5 in www.sciencedirect.com/science/arti...
July 28, 2025 at 6:14 PM
Distilled rule of thumb for optimal Protein:Trypsin ratio vs Total Protein concentration in bottom-up #proteomics:
Optimal protein:trypsin ≈ 0.1 * protein concentration (ng/uL)
Aligns well with standard guidelines e.g. 50:1 at ~500 ng/uL
Data from Table 5 in www.sciencedirect.com/science/arti...
Optimal protein:trypsin ≈ 0.1 * protein concentration (ng/uL)
Aligns well with standard guidelines e.g. 50:1 at ~500 ng/uL
Data from Table 5 in www.sciencedirect.com/science/arti...
Distilled rule of thumb for optimal Cosine cutoffs at various n matching MS2 fragments in #metabolomics ID:
Cosine > 0.90 - 0.05*MMP
Aligns well with ~80% at 3 matched fragments I learned before.
From Fig4a here www.nature.com/articles/s41...
Cosine > 0.90 - 0.05*MMP
Aligns well with ~80% at 3 matched fragments I learned before.
From Fig4a here www.nature.com/articles/s41...
July 24, 2025 at 7:49 PM
Distilled rule of thumb for optimal Cosine cutoffs at various n matching MS2 fragments in #metabolomics ID:
Cosine > 0.90 - 0.05*MMP
Aligns well with ~80% at 3 matched fragments I learned before.
From Fig4a here www.nature.com/articles/s41...
Cosine > 0.90 - 0.05*MMP
Aligns well with ~80% at 3 matched fragments I learned before.
From Fig4a here www.nature.com/articles/s41...