Woah, super cool! A class in how to piece apart metabolism in an enrichment culture, super interesting, and raises new questions of its own. Are you willing to speculate if there are any other lithotrophic metabolisms that involve syntrophy?

Yup the review I shared says this about terminal oxidases.

Perchlorate-reducing bacteria with Cld (super interesting, which I studied!) have so far always been facultative aerobes. Some obligate anaerobes without Cld can reduce perchlorate but ClO2 is removed chemically and no O2 is produced.

Well, GenomeSPOT uses amino acid frequency, so we think it reflects selection for aerobic or anaerobic metabolism. For gene-based, it depends. They can use O2-utilizing and O2-sensitive genes instead of just Cco, SOD, etc. One example: doi.org/10.1016/j.fr...

Yes! They use those enzymes to deplete (detoxify) oxygen, but have other enzymes that are sensitive to oxygen.

See: doi.org/10.1038/s415...
Possibly some genome annotations here: doi.org/10.1101/2023...

Very cool work. "The absence of cell cycle regulation genes may ... [predict] upper bounds on growth rates obtainable by these organisms" is a cool thought - we're all sick of not knowing *why* some taxa are harder to cultivate! Do you plan to expand on the prelim analysis?

For way more on the very interesting element chlorine, see my short review here: doi.org/10.1111/gbi....

2/2 HOCl (bleach) is oxidized Cl produced by our immune system, but also seems impactful in various oxic habitats. HOCl is understudied, and there seems to be a lot left to learn about how microbes adapt to oxidative stress from Cl.

Some comparative genomics: doi.org/10.1038/s413...

1/2 The broader DMSO reductase family is massive and catalyzes key parts of various biogeochemical cycles (N, Cl, I, Ar, Se, etc.). The majority of this family has unknown functions -- yet these enzymes often support respiration, making it easier to assay substrates.

doi.org/10.1128/spec...

I got a 404 webpage not found. You have an extra "n" at the end of your link. Fixed link: asm.org/climatesolutions

(Thanks for sharing!)