Tom Raaymakers
@tmraaymakers.bsky.social
Molecular phytopathologist at the Dutch National Plant Protection Organization/Netherlands Institute for Vectors, Invasive plants and Plant health (NIVIP)
In my previous lab this method was often used as a cheap alternative to commercial kits: bmcresnotes.biomedcentral.com/articles/10.... but don't know how well it would translate to your samples
DNA-free RNA isolation protocols for Arabidopsis thaliana, including seeds and siliques - BMC Research Notes
Background High throughput applications of the reverse transcriptase quantitative PCR (RT-qPCR) for quantification of gene expression demand straightforward procedures to isolate and analyze a conside...
bmcresnotes.biomedcentral.com
October 23, 2025 at 6:27 PM
In my previous lab this method was often used as a cheap alternative to commercial kits: bmcresnotes.biomedcentral.com/articles/10.... but don't know how well it would translate to your samples
Congratulations!
September 18, 2025 at 7:09 PM
Congratulations!
Yeah it's become so easy to get high quality (bacterial) genomes now. Fungal genomes are a bit more tricky I guess. Especially those pesky rusts.
August 25, 2025 at 7:25 PM
Yeah it's become so easy to get high quality (bacterial) genomes now. Fungal genomes are a bit more tricky I guess. Especially those pesky rusts.
Sure, but it’s still early days and this was the first bacterial plant pathogen sequenced (as far as I know, though some Xanthomonads and Xylella appeared soon after). We plant pathologists do crave a bit of recognition! 😉
August 25, 2025 at 7:17 PM
Sure, but it’s still early days and this was the first bacterial plant pathogen sequenced (as far as I know, though some Xanthomonads and Xylella appeared soon after). We plant pathologists do crave a bit of recognition! 😉
Also 4 plant pathogens in the top 100 seems about right 🙃
August 25, 2025 at 7:11 PM
Also 4 plant pathogens in the top 100 seems about right 🙃
Still being in the top 30 out of almost 3 million bacterial genomes available now ain't bad 😁
August 25, 2025 at 7:06 PM
Still being in the top 30 out of almost 3 million bacterial genomes available now ain't bad 😁
+the green revolution
August 23, 2025 at 5:27 PM
+the green revolution
Ruige zegge (Carex hirta)?
June 1, 2025 at 7:22 PM
Ruige zegge (Carex hirta)?
Reposted by Tom Raaymakers
Just in time to add it to our manuscript (hopefully submitted soon 🙂)
May 21, 2025 at 5:12 AM
Just in time to add it to our manuscript (hopefully submitted soon 🙂)
It is! Almost instant as opposed to hours previously 😃
May 19, 2025 at 7:45 AM
It is! Almost instant as opposed to hours previously 😃
I have raised the issue on the pyani-plus git, also a question about the number of log files, which maybe by design :)
May 19, 2025 at 7:44 AM
I have raised the issue on the pyani-plus git, also a question about the number of log files, which maybe by design :)