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tirthabanerjee.bsky.social
@tirthabanerjee.bsky.social
Happy to share that the preprint of our updated spatial mRNA multiplexing using RAMFISH (v2.0) is out... www.biorxiv.org/content/10.1...

Special thanks to all the collaborators for their efforts.
October 19, 2025 at 6:04 PM
Excited to share that our Geneplexer and Multiplexer systems can now achieve spatial mRNA signal detection within 4.5 hrs, including hybridization, secondary amplification, and washes.

Image: A z-slice (~5 µm) of a whole 14 dpf WT zf larva stained against markers nrp1a, gad2, and neuroD.
August 17, 2025 at 9:27 AM
Happy to collaborate with Ajay Mathuru's team at NUS. "Role of chrna5 in multi-substance preference and phenotypes comorbid with the development of substance dependence." Congratulations to the co-first authors, Tanisha Goel and Joshua Raine. www.biorxiv.org/content/10.1...
July 16, 2025 at 6:08 AM
Happy to share that our recent Bicyclus larval brain multiplex-FISH paper has been featured on the cover of Methods and Protocols.

www.mdpi.com/2409-9279/8/2
April 10, 2025 at 3:15 PM
Happy to share our latest 3D whole-mount multiplex-RNA brain scans of the larval brains of Bicyclus anynana at single-cell resolution...
We mapped multiple targets at single-cell resolution and made the raw data open source for anyone who would like to analyse it.
www.mdpi.com/2409-9279/8/...
March 14, 2025 at 12:41 PM
Our new manuscript describing gene multiplexing (using RAM-FISH) is finally out... We profiled multiple known and novel mRNA candidates during different developmental stages of Bicyclus and zebrafish using the easy-to-use HCR-based protocol that most labs can adapt. www.biorxiv.org/content/10.1...
December 12, 2024 at 8:39 PM