Hmmm... Might just be that cell. Here's another example of same experiment (from stasevichlab.colostate.edu)

Here’s a cool movie showing histone H3K27ac increase upon TSA treatment (inhibits deacetylation). Here the intrabody (green; anti-H3K27ac scFv-sfGFP) translocates into the nucleus as acetylation levels there increase.

Making intrabodies from antibodies just got easier! Learn how we made 𝟭𝟵 intrabodies to bind and light up peptides and histone modifications in live cells. And thanks to Academia, all sequences are freely available. (video credit: Yuko Sato @YukoSatoT2) (1/15)
www.biorxiv.org/content/10.1...