Tia Fletcher
@tiaxfletch.bsky.social
Xenopus Biologist researching all things, frogs, gene editing and rare disease.
Reposted by Tia Fletcher
If you want to study a genetic disorder of growth, in a non-mammalian model system, Xenopus 🐸 has a unique advantage. As we show with Recql4 in G3 this month, it is possible to knock out a gene with CRISPR on just one side of the animal doi.org/10.1093/g3jo... 🧪
Unilateral loss of recql4 function in Xenopus laevis tadpoles leads to ipsilateral ablation of the forelimb, hypoplastic Meckel's cartilage, and vascular defects
Abstract. RECQL4 encodes a RecQ helicase, one of a family of DNA unwinding enzymes with roles in DNA replication, double-strand break repair, and genomic s
doi.org
October 9, 2025 at 10:09 AM
If you want to study a genetic disorder of growth, in a non-mammalian model system, Xenopus 🐸 has a unique advantage. As we show with Recql4 in G3 this month, it is possible to knock out a gene with CRISPR on just one side of the animal doi.org/10.1093/g3jo... 🧪