Tara Essock-Burns
banner
taraeb.bsky.social
Tara Essock-Burns
@taraeb.bsky.social
[6/6] Thanks to all collaborators on this team effort! If you want to read more about our strategy when it comes to microscopy tools
@arcadiascience.bsky.social, then check out our project narrative. 🔬
research.arcadiascience.com/microscopy
Microscopy: Visually interrogating the natural world
research.arcadiascience.com
November 15, 2024 at 12:07 AM
[5/6] Let us know if you try SwimTracker on your favorite cells. We’d like to know how it works for different types of swimming and other forms of motility. Please comment on the pub with your findings! 👇
research.arcadiascience.com/pub/resource...
A high-throughput imaging approach to track and quantify single-cell swimming
Live imaging of swimming cells can yield insight into an organism’s viability and responses to environmental stimuli. We developed a microscopy workflow and image analysis pipeline, SwimTracker, to tr...
research.arcadiascience.com
November 15, 2024 at 12:07 AM
[4/6] While both vessel types we tried work for capturing algal swimming, cells swim differently, whether they’re in confined microchambers or in larger wells of plates! We go through some of the trade-offs for each vessel and give recommendations for designing a motility experiment in the pub.
a dog swimming in a pool with the words look i 'm swimming above it
ALT: a dog swimming in a pool with the words look i 'm swimming above it
media.tenor.com
November 15, 2024 at 12:07 AM
[3/6] SwimTracker is a Python package that generates trajectories and calculates motility metrics for downstream analyses. Check out GitHub for the code and accompanying notebooks where we show examples of multi-dimensional analyses you can try doing on the metrics.
github.com/Arcadia-Scie...
GitHub - Arcadia-Science/2024-unicellular-tracking at v1.1
Image analysis workflows for tracking unicellular organisms - GitHub - Arcadia-Science/2024-unicellular-tracking at v1.1
github.com
November 15, 2024 at 12:07 AM
[2/6] We wanted a high-throughput microscopy workflow to quickly capture and compare swimming dynamics across groups of interest. So we acquired time-lapse videos of unicellular algae in two vessel types, agar microchambers and microtiter plates. Then comes SwimTracker!
November 15, 2024 at 12:07 AM