Takumi Higaki
@takumihigaki.bsky.social
Prof.@Lab. for Imaging Biology@FAST/IRCAEB, Kumamoto Univ.
Studying plant cell biology through microscopic image analysis, with a focus on cellular structures and dynamics.
Studying plant cell biology through microscopic image analysis, with a focus on cellular structures and dynamics.
Our review on computational approaches to plant cytoskeleton dynamics is now available in Cytoskeleton.
We cover both classical methods and recent deep learning-based analyses.
🔗 onlinelibrary.wiley.com/doi/10.1002/...
#ComputationalBiology #PlantCytoskeleton #Microscopy
We cover both classical methods and recent deep learning-based analyses.
🔗 onlinelibrary.wiley.com/doi/10.1002/...
#ComputationalBiology #PlantCytoskeleton #Microscopy
Computational Approaches to Revisiting Plant Cytoskeleton Organization and Dynamics
Live-cell imaging has enabled the visualization of cytoskeletal dynamics with high spatiotemporal resolution, producing vast, and complex datasets. Recent advancements in live-cell imaging techniques...
onlinelibrary.wiley.com
June 12, 2025 at 10:44 AM
Our review on computational approaches to plant cytoskeleton dynamics is now available in Cytoskeleton.
We cover both classical methods and recent deep learning-based analyses.
🔗 onlinelibrary.wiley.com/doi/10.1002/...
#ComputationalBiology #PlantCytoskeleton #Microscopy
We cover both classical methods and recent deep learning-based analyses.
🔗 onlinelibrary.wiley.com/doi/10.1002/...
#ComputationalBiology #PlantCytoskeleton #Microscopy
🌱Thrilled to share our latest study using 4D phenotyping to explore how MYOSIN XI shapes branch growth direction in Arabidopsis.
Now published in Quantitative Plant Biology!
🔗 doi.org/10.1017/qpb....
#PlantBiology #4Dphenotyping #Cytoskeleton
Now published in Quantitative Plant Biology!
🔗 doi.org/10.1017/qpb....
#PlantBiology #4Dphenotyping #Cytoskeleton
Four-dimensional phenotyping reveals MYOSIN XI-dependent establishment of branch morphology through upward- and stably-directed growth in Arabidopsis | Quantitative Plant Biology | Cambridge Core
Four-dimensional phenotyping reveals MYOSIN XI-dependent establishment of branch morphology through upward- and stably-directed growth in Arabidopsis - Volume 6
doi.org
June 12, 2025 at 10:44 AM
🌱Thrilled to share our latest study using 4D phenotyping to explore how MYOSIN XI shapes branch growth direction in Arabidopsis.
Now published in Quantitative Plant Biology!
🔗 doi.org/10.1017/qpb....
#PlantBiology #4Dphenotyping #Cytoskeleton
Now published in Quantitative Plant Biology!
🔗 doi.org/10.1017/qpb....
#PlantBiology #4Dphenotyping #Cytoskeleton
Our new paper is out in BBRC.
We investigated how glycyrrhetinic acid induces lipid peroxidation-related cell death in tobacco BY-2 cells.
🔗 doi.org/10.1016/j.bb...
#PlantCellDeath #NaturalProducts #BY2cells
We investigated how glycyrrhetinic acid induces lipid peroxidation-related cell death in tobacco BY-2 cells.
🔗 doi.org/10.1016/j.bb...
#PlantCellDeath #NaturalProducts #BY2cells
Redirecting
doi.org
June 12, 2025 at 10:44 AM
Our new paper is out in BBRC.
We investigated how glycyrrhetinic acid induces lipid peroxidation-related cell death in tobacco BY-2 cells.
🔗 doi.org/10.1016/j.bb...
#PlantCellDeath #NaturalProducts #BY2cells
We investigated how glycyrrhetinic acid induces lipid peroxidation-related cell death in tobacco BY-2 cells.
🔗 doi.org/10.1016/j.bb...
#PlantCellDeath #NaturalProducts #BY2cells
New work published in Plant Cell Reports.
We used deep learning-based image restoration to visualize actin microfilaments during early cell plate formation in plant cells.
🔗 doi.org/10.1007/s002...
#DeepLearning #PlantCytokinesis #ImageAnalysis
We used deep learning-based image restoration to visualize actin microfilaments during early cell plate formation in plant cells.
🔗 doi.org/10.1007/s002...
#DeepLearning #PlantCytokinesis #ImageAnalysis
Distinct actin microfilament localization during early cell plate formation through deep learning-based image restoration - Plant Cell Reports
Key message Using deep learning-based image restoration, we achieved high-resolution 4D imaging with minimal photodamage, revealing distinct localization and suggesting Lifeact-RFP-labeled actin micro...
doi.org
June 12, 2025 at 10:43 AM
New work published in Plant Cell Reports.
We used deep learning-based image restoration to visualize actin microfilaments during early cell plate formation in plant cells.
🔗 doi.org/10.1007/s002...
#DeepLearning #PlantCytokinesis #ImageAnalysis
We used deep learning-based image restoration to visualize actin microfilaments during early cell plate formation in plant cells.
🔗 doi.org/10.1007/s002...
#DeepLearning #PlantCytokinesis #ImageAnalysis
Reposted by Takumi Higaki
IN BRIEF: Dawning aurora: TPXL3 activates α-Aurora kinase and regulates mitotic spindle morphogenesis (Renuka Kolli) doi.org/10.1093/plce... #PlantScience
doi.org
April 18, 2025 at 3:13 PM
IN BRIEF: Dawning aurora: TPXL3 activates α-Aurora kinase and regulates mitotic spindle morphogenesis (Renuka Kolli) doi.org/10.1093/plce... #PlantScience
Reposted by Takumi Higaki
The unconventional TPX2 family protein TPXL3 regulates α Aurora kinase function in spindle morphogenesis in Arabidopsis (Xingguang Deng, Takumi Higaki, Hong-Hui Lin, Yuh-Ru Julie Lee, Bo Liu) academic.oup.com/plcell/advan... #PlantScience
academic.oup.com
April 1, 2025 at 2:06 PM
The unconventional TPX2 family protein TPXL3 regulates α Aurora kinase function in spindle morphogenesis in Arabidopsis (Xingguang Deng, Takumi Higaki, Hong-Hui Lin, Yuh-Ru Julie Lee, Bo Liu) academic.oup.com/plcell/advan... #PlantScience
🧵 New paper! Our study in The Plant Cell reveals a key role for TPXL3, an unconventional TPX2 family protein, in mitotic spindle morphogenesis in Arabidopsis 🌱✨
We show that TPXL3 is essential for targeting and activating α Aurora kinase on spindle microtubules. 🧬🌀
📖 doi.org/10.1093/plce...
We show that TPXL3 is essential for targeting and activating α Aurora kinase on spindle microtubules. 🧬🌀
📖 doi.org/10.1093/plce...
pendingpublications
Pending Publication
doi.org
March 31, 2025 at 12:37 AM
🧵 New paper! Our study in The Plant Cell reveals a key role for TPXL3, an unconventional TPX2 family protein, in mitotic spindle morphogenesis in Arabidopsis 🌱✨
We show that TPXL3 is essential for targeting and activating α Aurora kinase on spindle microtubules. 🧬🌀
📖 doi.org/10.1093/plce...
We show that TPXL3 is essential for targeting and activating α Aurora kinase on spindle microtubules. 🧬🌀
📖 doi.org/10.1093/plce...
📰✨ New Press Release! ✨📰
Researchers at Kumamoto University have developed an AI-powered technique to analyze cytoskeletal structures with high precision! 🤖🔬💡
🔗 Read more here: www.eurekalert.org/news-release...
#Cytoskeleton #DeepLearning #AI #Microscopy #ScienceNews
Researchers at Kumamoto University have developed an AI-powered technique to analyze cytoskeletal structures with high precision! 🤖🔬💡
🔗 Read more here: www.eurekalert.org/news-release...
#Cytoskeleton #DeepLearning #AI #Microscopy #ScienceNews
Deep learning revolutionizes cytoskeleton research
A research team at Kumamoto University has developed a groundbreaking deep learning-based method for analyzing the cytoskeleton—the structural framework inside cells—more accurately and ef...
www.eurekalert.org
March 19, 2025 at 6:36 AM
📰✨ New Press Release! ✨📰
Researchers at Kumamoto University have developed an AI-powered technique to analyze cytoskeletal structures with high precision! 🤖🔬💡
🔗 Read more here: www.eurekalert.org/news-release...
#Cytoskeleton #DeepLearning #AI #Microscopy #ScienceNews
Researchers at Kumamoto University have developed an AI-powered technique to analyze cytoskeletal structures with high precision! 🤖🔬💡
🔗 Read more here: www.eurekalert.org/news-release...
#Cytoskeleton #DeepLearning #AI #Microscopy #ScienceNews
🌿🔬 New paper! Our study in Plant Mol Biol presents label-free virtual staining for plant cells! 🚀 Using bright-field microscopy and AI, we enable quantitative imaging without fluorescent dyes. 🌱✨
📄 doi.org/10.1007/s111...
#PlantBiology #Microscopy #DeepLearning #VirtualStaining
📄 doi.org/10.1007/s111...
#PlantBiology #Microscopy #DeepLearning #VirtualStaining
Virtual staining from bright-field microscopy for label-free quantitative analysis of plant cell structures - Plant Molecular Biology
The applicability of a deep learning model for the virtual staining of plant cell structures using bright-field microscopy was investigated. The training dataset consisted of microscopy images of toba...
doi.org
March 18, 2025 at 9:25 AM
🌿🔬 New paper! Our study in Plant Mol Biol presents label-free virtual staining for plant cells! 🚀 Using bright-field microscopy and AI, we enable quantitative imaging without fluorescent dyes. 🌱✨
📄 doi.org/10.1007/s111...
#PlantBiology #Microscopy #DeepLearning #VirtualStaining
📄 doi.org/10.1007/s111...
#PlantBiology #Microscopy #DeepLearning #VirtualStaining
Horiuchi et al. (2024) - AI for cytoskeleton analysis
🧬📊 In Protoplasma, we introduce AI-based segmentation for high-throughput cytoskeleton analysis! 🚀 This approach enables precise, efficient quantification of cytoskeletal density. 🌱🔍
🔗 doi.org/10.1007/s007...
#DeepLearning #Cytoskeleton
🧬📊 In Protoplasma, we introduce AI-based segmentation for high-throughput cytoskeleton analysis! 🚀 This approach enables precise, efficient quantification of cytoskeletal density. 🌱🔍
🔗 doi.org/10.1007/s007...
#DeepLearning #Cytoskeleton
Deep learning-based cytoskeleton segmentation for accurate high-throughput measurement of cytoskeleton density - Protoplasma
Microscopic analyses of cytoskeleton organization are crucial for understanding various cellular activities, including cell proliferation and environmental responses in plants. Traditionally, assessme...
doi.org
March 18, 2025 at 9:24 AM
Horiuchi et al. (2024) - AI for cytoskeleton analysis
🧬📊 In Protoplasma, we introduce AI-based segmentation for high-throughput cytoskeleton analysis! 🚀 This approach enables precise, efficient quantification of cytoskeletal density. 🌱🔍
🔗 doi.org/10.1007/s007...
#DeepLearning #Cytoskeleton
🧬📊 In Protoplasma, we introduce AI-based segmentation for high-throughput cytoskeleton analysis! 🚀 This approach enables precise, efficient quantification of cytoskeletal density. 🌱🔍
🔗 doi.org/10.1007/s007...
#DeepLearning #Cytoskeleton
Hitora et al. (2024) - AI in drug screening
🧪🤖 Our work in J Nat Prod applies machine learning to screen osteoclast differentiation inhibitors from natural products! AI accelerates drug discovery for bone-related diseases. 🦴🔬
🔗 doi.org/10.1021/acs....
#DrugDiscovery #MachineLearning #Osteoclast
🧪🤖 Our work in J Nat Prod applies machine learning to screen osteoclast differentiation inhibitors from natural products! AI accelerates drug discovery for bone-related diseases. 🦴🔬
🔗 doi.org/10.1021/acs....
#DrugDiscovery #MachineLearning #Osteoclast
March 18, 2025 at 9:22 AM
Hitora et al. (2024) - AI in drug screening
🧪🤖 Our work in J Nat Prod applies machine learning to screen osteoclast differentiation inhibitors from natural products! AI accelerates drug discovery for bone-related diseases. 🦴🔬
🔗 doi.org/10.1021/acs....
#DrugDiscovery #MachineLearning #Osteoclast
🧪🤖 Our work in J Nat Prod applies machine learning to screen osteoclast differentiation inhibitors from natural products! AI accelerates drug discovery for bone-related diseases. 🦴🔬
🔗 doi.org/10.1021/acs....
#DrugDiscovery #MachineLearning #Osteoclast