and picnic-ing...

Relaxing on the rooftop, an evening picnic with students and instructors

Taking us through axial elongation is David Turner @gastruloids.bsky.social, a great teacher of ES cell handling and formulating protocols for reproducible gastruloid generation. A-P extension, and into the 2nd week.

Isolation of small intestine and colon crypts to make organoids with Giulia Nigro, Aline Stedman, Sophie Dulauroy - following a great lecture by Marc Van de Wetering into the history and organoid biology of the gut. Great exercise on how to handle samples with the utmost care.

Isolation of muscle fibres and their stem (satellite) cells. Plate and wait, from quiescence to differentiation - following Shahragim’s lecture on muscle stem cell emergence and diversity. @tajbakhshlab.bsky.social,
@brendan-evano.bsky.social, Barbara Gayraud-Morel. Patience is a virtue.

1st week ends with: a swim in the zebrafish brain - cool genetic tracing of neural stem cells with @zenlabpasteur.bsky.social, Laure Bally-Cuif, Sara Ortica, Tanya Foley, Isabelle Foucher—lots covered in a condensed period.

Pluripotency, CRISPR/Cas9, organoid days. Lectures to bench. With a precious and passionate lecture by Michele De Luca

Education… so important. Second day of the #ASCB course @institutpasteur with @LabTajbakhsh @zenlabpasteur @bgayraudmorel and a great group of interactive students. Once again with the irreplaceable trio: Laurence Daheron, Frank Yates, Michel Cohen-Tannoudji!