Surprisingly, expression analysis indicated that subset of Adm+ neurons expressing Uts2b+ were activated only by bladder distension. Can nociceptors be indeed organ-selective? We used a Uts2b:Cre line to confirm that projections of Uts2b+ nociceptors are confined to the bladder. 6/8

This is hard to study because it’s difficult to see which combination of nociceptor classes is activated in vivo during noxious stimulation. We used the calcium integrator CaMPARI to label stimulus-activated neurons, allowing us to profile nociceptive ensembles with single-cell RNA-seq. 2/8