synbiosean.bsky.social
@synbiosean.bsky.social
You can split them in two parts. If I’m using Golden Gate for final constructs, I usually do this when ordering clonal DNA so I don’t have to worry about toxicity until everything is assembled. Use NEBs Ligase Fidelity tools to help with junctions.
April 25, 2025 at 1:44 PM
Try adding a bit more glucose to the plates to activate glucose repression.
March 28, 2025 at 8:10 PM