Lukas M.
@sunsetboy22.bsky.social
Microbial Ecology/Wheat/Virology at AIT. Vienna.
Ah, interesting! If the amplicon data comes from a complex sample, the different ASV proportions could result from non-isolated strains in the microbiome sample, sharing one V4 copy, but not the other and therefore skewing the V4-copy-ratios? then, the least relabund ASV would be the most realistic?
September 19, 2025 at 9:29 PM
Ah, interesting! If the amplicon data comes from a complex sample, the different ASV proportions could result from non-isolated strains in the microbiome sample, sharing one V4 copy, but not the other and therefore skewing the V4-copy-ratios? then, the least relabund ASV would be the most realistic?
Once, I just used vmatchPattern to find the ASV fasta in the isolate fasta. Theoretically, different 16S sequences of the same bug should yield different ASVs. Or do I not get the problem?
September 19, 2025 at 5:09 PM
Once, I just used vmatchPattern to find the ASV fasta in the isolate fasta. Theoretically, different 16S sequences of the same bug should yield different ASVs. Or do I not get the problem?