Sunil Kumar Pradhan
@sunil-k-pradhan.bsky.social
PhD student in genome replication and epigenetic dynamics
We finally reveal a strand-specific role of MajSat RNA by using forward / reverse strand-specific locked nucleic acid DNA gapmers. We found the forward MajSat RNA to be regulating the replication timing of the PCH, suggesting ncRNA as regulators of replication timing.
September 10, 2025 at 8:50 AM
We finally reveal a strand-specific role of MajSat RNA by using forward / reverse strand-specific locked nucleic acid DNA gapmers. We found the forward MajSat RNA to be regulating the replication timing of the PCH, suggesting ncRNA as regulators of replication timing.
Loss of H3K36me3 at pericentromeric heterochromatin disrupts transcription:
↑ RNA Pol II Ser5 (initiation)
↓ RNA Pol II Ser2 (elongation)
↓ Major satellite RNA levels
Together, these changes point to transcriptional stalling and loss of MajSat RNA.
↑ RNA Pol II Ser5 (initiation)
↓ RNA Pol II Ser2 (elongation)
↓ Major satellite RNA levels
Together, these changes point to transcriptional stalling and loss of MajSat RNA.
September 10, 2025 at 8:50 AM
Loss of H3K36me3 at pericentromeric heterochromatin disrupts transcription:
↑ RNA Pol II Ser5 (initiation)
↓ RNA Pol II Ser2 (elongation)
↓ Major satellite RNA levels
Together, these changes point to transcriptional stalling and loss of MajSat RNA.
↑ RNA Pol II Ser5 (initiation)
↓ RNA Pol II Ser2 (elongation)
↓ Major satellite RNA levels
Together, these changes point to transcriptional stalling and loss of MajSat RNA.
When we depleted H3K36me3 specifically from pericentromeric heterochromatin, replication timing was disrupted.
⚡ Instead of a neatly ordered sequence, PCH began replicating together with other heterochromatic regions - a loss of temporal control linked to genome instability.
⚡ Instead of a neatly ordered sequence, PCH began replicating together with other heterochromatic regions - a loss of temporal control linked to genome instability.
September 10, 2025 at 8:50 AM
When we depleted H3K36me3 specifically from pericentromeric heterochromatin, replication timing was disrupted.
⚡ Instead of a neatly ordered sequence, PCH began replicating together with other heterochromatic regions - a loss of temporal control linked to genome instability.
⚡ Instead of a neatly ordered sequence, PCH began replicating together with other heterochromatic regions - a loss of temporal control linked to genome instability.
To probe the role of H3K36me3 at PCH, we developed a new approach:
✨ Nanobody-mediated targeting of oncohistone H3.1 K36M directly to pericentromeric heterochromatin.
This locally reduced H3K36me3 without altering other histone modifications, and elsewhere in the chromatin.
✨ Nanobody-mediated targeting of oncohistone H3.1 K36M directly to pericentromeric heterochromatin.
This locally reduced H3K36me3 without altering other histone modifications, and elsewhere in the chromatin.
September 10, 2025 at 8:50 AM
To probe the role of H3K36me3 at PCH, we developed a new approach:
✨ Nanobody-mediated targeting of oncohistone H3.1 K36M directly to pericentromeric heterochromatin.
This locally reduced H3K36me3 without altering other histone modifications, and elsewhere in the chromatin.
✨ Nanobody-mediated targeting of oncohistone H3.1 K36M directly to pericentromeric heterochromatin.
This locally reduced H3K36me3 without altering other histone modifications, and elsewhere in the chromatin.
Could I be added? Thanks!
December 10, 2024 at 11:32 AM
Could I be added? Thanks!