Structura Biotechnology
@structurabio.bsky.social
Algorithms, Software and Systems for Cryo-EM. Makers of CryoSPARC.
At cryosparc.com/automated-workflows we provide instructions and CryoSPARC Workflow JSON files so that users can get started with automation easily.
Reach out at discuss.cryosparc.com/c/automated-workflows.
Happy processing!
Reach out at discuss.cryosparc.com/c/automated-workflows.
Happy processing!
CryoSPARC | Automated Repeat‑Target Structure Determination
An end‑to‑end, automated workflow for single‑particle cryo‑EM that achieves state‑of‑the‑art consensus maps suitable for model building—demonstrated across 21 GPCR datasets, meeting or exceeding publi...
cryosparc.com
October 20, 2025 at 2:32 PM
At cryosparc.com/automated-workflows we provide instructions and CryoSPARC Workflow JSON files so that users can get started with automation easily.
Reach out at discuss.cryosparc.com/c/automated-workflows.
Happy processing!
Reach out at discuss.cryosparc.com/c/automated-workflows.
Happy processing!
Automated processing unlocks several benefits. Time-to-structure can be greatly reduced and throughput can be easily scaled for repeat-target use cases. Solving multiple structures per day can now become a reality. 🚀
October 20, 2025 at 2:32 PM
Automated processing unlocks several benefits. Time-to-structure can be greatly reduced and throughput can be easily scaled for repeat-target use cases. Solving multiple structures per day can now become a reality. 🚀
The workflow is powered by new methods, including micrograph junk detection, denoising, and reference based auto selection of 2D classes and 3D volumes. Read the preprint for more insights and details.
October 20, 2025 at 2:32 PM
The workflow is powered by new methods, including micrograph junk detection, denoising, and reference based auto selection of 2D classes and 3D volumes. Read the preprint for more insights and details.
The automated workflow generalizes easily to new target classes.
We provide downloadable #CryoSPARC Workflow JSON files so that users can replicate, adapt and extend it for their own targets. All required jobs and tools are already available in v4.7.1+.
We provide downloadable #CryoSPARC Workflow JSON files so that users can replicate, adapt and extend it for their own targets. All required jobs and tools are already available in v4.7.1+.
October 20, 2025 at 2:32 PM
The automated workflow generalizes easily to new target classes.
We provide downloadable #CryoSPARC Workflow JSON files so that users can replicate, adapt and extend it for their own targets. All required jobs and tools are already available in v4.7.1+.
We provide downloadable #CryoSPARC Workflow JSON files so that users can replicate, adapt and extend it for their own targets. All required jobs and tools are already available in v4.7.1+.
In 10 of 21 cases, the automated results enable improved model building of the ligand, binding pocket, side chain positioning and/or extracellular loops. Three striking examples are shown here and in the first video.
October 20, 2025 at 2:32 PM
In 10 of 21 cases, the automated results enable improved model building of the ligand, binding pocket, side chain positioning and/or extracellular loops. Three striking examples are shown here and in the first video.
We test across 21 challenging active and inactive GPCR datasets.
Despite small particle size and the presence of contaminants, empty micelles, broken, denatured and aggregated particles, our automated workflow yields equal or better resolution and map quality in 17 of 21 cases.
Despite small particle size and the presence of contaminants, empty micelles, broken, denatured and aggregated particles, our automated workflow yields equal or better resolution and map quality in 17 of 21 cases.
October 20, 2025 at 2:32 PM
We test across 21 challenging active and inactive GPCR datasets.
Despite small particle size and the presence of contaminants, empty micelles, broken, denatured and aggregated particles, our automated workflow yields equal or better resolution and map quality in 17 of 21 cases.
Despite small particle size and the presence of contaminants, empty micelles, broken, denatured and aggregated particles, our automated workflow yields equal or better resolution and map quality in 17 of 21 cases.
The automation strategy we have developed can be set up once for any specific target class (e.g. active state GPCRs with Nb35) and reused to process multiple datasets fully hands-off. It makes use of minimal prior knowledge through a low-res (15Å) 3D reference map.
October 20, 2025 at 2:32 PM
The automation strategy we have developed can be set up once for any specific target class (e.g. active state GPCRs with Nb35) and reused to process multiple datasets fully hands-off. It makes use of minimal prior knowledge through a low-res (15Å) 3D reference map.