Stephan Wagner
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Stephan Wagner
@stephanwagner.bsky.social
230 followers 250 following 15 posts
Lecturer for microscopy 🔬 @UniBonn with key interests in biosensors and redox biology. 🌱 scientist. 🚲 lover.
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stephanwagner.bsky.social
A growing list of genetically encoded biosensors helps to shine light on plant metabolism. We reviewed - also for the non-expert - available probes and recent advances in the field:

www.sciencedirect.com/science/arti...
Scheme how genetically encoded biosensors can shine light on plant metabolism. Intrinsic sensors bind their analyte directly, extrensic sensors require a sensing domain fused to one, or more, fluorescent proteins. In paraplexing experiments, multiple sensors are individually expressed in plants and their responses are recorded in parallel. In multiplexing experiments, two or more sensors with non-overlapping excitation and emission spectra are expressed in the same plant. Sensor responses, either recorded in paraplexing or multiplexing experiments over time and through appropriate instruments, such as fluorescence microscopes or plate readers, can be used to create increasingly complex metabolic networks.
April 21, 2025 at 9:30 AM
stephanwagner.bsky.social
If you see this, quote with flowers from your gallery. We can all use some beauty right now. (Hydrangea macrophylla in Brittany, France)
January 28, 2025 at 4:40 PM