@stanleyqilab.bsky.social
We discovered and validated inflammaging-specific drivers including EIF2AK2, SERPING1, HMGCS2, CMKLR1. Inhibiting them blunts IL-6-associated senescence while maintaining cell identity.
August 6, 2025 at 5:54 PM
We discovered and validated inflammaging-specific drivers including EIF2AK2, SERPING1, HMGCS2, CMKLR1. Inhibiting them blunts IL-6-associated senescence while maintaining cell identity.
Two screens for two aging states: replicative senescence (RSS) and IL-6–driven inflammaging (ISS). Positive-selection CRISPRi reveals novel regulators you can modulate without breaking MSC identity to alter their aging state.
August 6, 2025 at 5:54 PM
Two screens for two aging states: replicative senescence (RSS) and IL-6–driven inflammaging (ISS). Positive-selection CRISPRi reveals novel regulators you can modulate without breaking MSC identity to alter their aging state.
CRISPR-TO-enabled screening in primary neurons identifies Stmn2 mRNA localization as a driver of neurite outgrowth.
May 26, 2025 at 1:02 AM
CRISPR-TO-enabled screening in primary neurons identifies Stmn2 mRNA localization as a driver of neurite outgrowth.
In primary cortical neurons, we demonstrate that repositioned mRNAs undergo local translation, which regulates neuronal morphology and axonal regeneration.
May 26, 2025 at 1:02 AM
In primary cortical neurons, we demonstrate that repositioned mRNAs undergo local translation, which regulates neuronal morphology and axonal regeneration.
CRISPR-TO effectively functions in primary neurons for ultra-long distance RNA transport, in which spatial transcriptomes are crucially linked to pathological processes.
May 26, 2025 at 1:02 AM
CRISPR-TO effectively functions in primary neurons for ultra-long distance RNA transport, in which spatial transcriptomes are crucially linked to pathological processes.
It allows for inducible and reversible bidirectional RNA transport along microtubules via motor proteins.
May 26, 2025 at 1:02 AM
It allows for inducible and reversible bidirectional RNA transport along microtubules via motor proteins.
CRISPR-TO enables targeted localization of endogenous RNAs to diverse subcellular compartments.
May 26, 2025 at 1:02 AM
CRISPR-TO enables targeted localization of endogenous RNAs to diverse subcellular compartments.
Spatial RNA organization has a pivotal role in diverse cellular processes and diseases. However, its functional implications remain largely unexplored due to limited technologies for perturbing endogenous RNA localization.
May 26, 2025 at 1:02 AM
Spatial RNA organization has a pivotal role in diverse cellular processes and diseases. However, its functional implications remain largely unexplored due to limited technologies for perturbing endogenous RNA localization.
Applying Oligo-LiveFISH to study enhancer-promoter interaction, we found that stimulating FOS transcription led to reduced 3D distance, increased confinement, and slower dynamics between the FOS enhancer and promoter.
April 16, 2025 at 11:42 PM
Applying Oligo-LiveFISH to study enhancer-promoter interaction, we found that stimulating FOS transcription led to reduced 3D distance, increased confinement, and slower dynamics between the FOS enhancer and promoter.
Combined with polymer-based dynamic modeling, Oligo-LiveFISH revealed two distinct modes of chromatin communication: 1D cis-communication, predominated at short distances (up to 300 kb), and 3D trans-communication, which is significant over long distances (> 1 Mb).
April 16, 2025 at 11:42 PM
Combined with polymer-based dynamic modeling, Oligo-LiveFISH revealed two distinct modes of chromatin communication: 1D cis-communication, predominated at short distances (up to 300 kb), and 3D trans-communication, which is significant over long distances (> 1 Mb).
Integrated with 3D super-localization microscopy, Oligo-LiveFISH tracked genomic regions at high spatial (20-nm) and temporal (50-ms) resolution, revealing highly sub-diffusive chromatin motion consistent with fractional Brownian motion.
April 16, 2025 at 11:41 PM
Integrated with 3D super-localization microscopy, Oligo-LiveFISH tracked genomic regions at high spatial (20-nm) and temporal (50-ms) resolution, revealing highly sub-diffusive chromatin motion consistent with fractional Brownian motion.
Oligo-LiveFISH enables the tracking of chromatin dynamics in diverse cell types, including hard-to-image cells such as primary T cells and induced neurons (iNs) derived from human embryonic stem cells.
April 16, 2025 at 11:41 PM
Oligo-LiveFISH enables the tracking of chromatin dynamics in diverse cell types, including hard-to-image cells such as primary T cells and induced neurons (iNs) derived from human embryonic stem cells.
Utilizing machine learning, we characterized key parameters of chromatin and gRNA features on imaging efficiency and established gRNA design principles for live chromatin imaging.
April 16, 2025 at 11:40 PM
Utilizing machine learning, we characterized key parameters of chromatin and gRNA features on imaging efficiency and established gRNA design principles for live chromatin imaging.
To address this, we exploited approaches to generate effective gRNA pools to image non-repetitive loci by computational design, in vitro transcription, and chemical labeling, delivered as ribonucleoproteins.
April 16, 2025 at 11:40 PM
To address this, we exploited approaches to generate effective gRNA pools to image non-repetitive loci by computational design, in vitro transcription, and chemical labeling, delivered as ribonucleoproteins.
Oligo-LiveFISH is a high-resolution, reagent-based platform for studying 3D genome dynamics and their links to cellular processes in diverse cell types, including primary cells.
www.cell.com/cell/fulltex...
authors.elsevier.com/c/1kxK7L7PXu...
www.cell.com/cell/fulltex...
authors.elsevier.com/c/1kxK7L7PXu...
April 16, 2025 at 11:39 PM
Oligo-LiveFISH is a high-resolution, reagent-based platform for studying 3D genome dynamics and their links to cellular processes in diverse cell types, including primary cells.
www.cell.com/cell/fulltex...
authors.elsevier.com/c/1kxK7L7PXu...
www.cell.com/cell/fulltex...
authors.elsevier.com/c/1kxK7L7PXu...
Our study establishes trogocytosis as a novel, programmable framework for cell-based macromolecular delivery. We look forward to seeing further applications enabled by the technology!
March 20, 2025 at 6:03 PM
Our study establishes trogocytosis as a novel, programmable framework for cell-based macromolecular delivery. We look forward to seeing further applications enabled by the technology!
TRANSFER demonstrated superior programmability by (1) recognizing a combination of cell surface markers, and mediating delivery under AND gate logic; (2) being applicable to the delivery of Cre and ZFN for gene editing, and prodrug-converting enzymes for targeted cell ablation.
March 20, 2025 at 6:02 PM
TRANSFER demonstrated superior programmability by (1) recognizing a combination of cell surface markers, and mediating delivery under AND gate logic; (2) being applicable to the delivery of Cre and ZFN for gene editing, and prodrug-converting enzymes for targeted cell ablation.
To expand trogocytosis beyond surface molecules, we introduced a pH-sensitive cleavable linker, enabling the translocation of membrane-recruited Cas9 cargos to the nucleus upon transfer. We termed the system trogocytosis-based transfer and functional effector release (TRANSFER).
March 20, 2025 at 6:02 PM
To expand trogocytosis beyond surface molecules, we introduced a pH-sensitive cleavable linker, enabling the translocation of membrane-recruited Cas9 cargos to the nucleus upon transfer. We termed the system trogocytosis-based transfer and functional effector release (TRANSFER).
However, trogocytosed molecules were found to be unfunctional in recipient cells. To tackle this challenge, we engineered donor cells with pH-sensitive membrane fusogens, which get co-transferred to recipient cells through trogocytosis and mediate endosomal escape of cargos.
March 20, 2025 at 6:01 PM
However, trogocytosed molecules were found to be unfunctional in recipient cells. To tackle this challenge, we engineered donor cells with pH-sensitive membrane fusogens, which get co-transferred to recipient cells through trogocytosis and mediate endosomal escape of cargos.
We were inspired by trogocytosis, where cells transfer membrane fragments and associated molecules in a contact-dependent manner. We showed that donor cells with engineered receptors can transfer surface molecules in a ligand-specific manner through direct cell-cell contact.
March 20, 2025 at 6:01 PM
We were inspired by trogocytosis, where cells transfer membrane fragments and associated molecules in a contact-dependent manner. We showed that donor cells with engineered receptors can transfer surface molecules in a ligand-specific manner through direct cell-cell contact.