Carter Brzezinski
@sp3intermediate.bsky.social
Postdoc - Biegasiewicz lab @ Emory University. PhD - Wuest Lab. Kenyon College Alumni. Loves chemoenzymatic synthesis, natural products, and antibiotic drug discovery. 🇯🇵
no worries! this one has been haunting me for a bit. thanks for sharing it!
August 5, 2025 at 12:45 PM
no worries! this one has been haunting me for a bit. thanks for sharing it!
might have missed it but did you post a solution for this one?
August 5, 2025 at 11:06 AM
might have missed it but did you post a solution for this one?
Chicago: if you’re around the southside, the point is a beautiful park around the lake. Also Strings is an amazing ramen place in Chinatown - very easily accessible from the train
July 5, 2025 at 4:20 PM
Chicago: if you’re around the southside, the point is a beautiful park around the lake. Also Strings is an amazing ramen place in Chinatown - very easily accessible from the train
Lots more on this project that I cant share quite yet, but special thanks to the Sieber lab at TUM for hosting me in Munich to learn and perform these chemoproteomic experiments! Lipoprotein trafficking is clearly an attractive target for gram(-)’s, especially for beta-lactamase expressing bacteria.
June 29, 2025 at 5:36 PM
Lots more on this project that I cant share quite yet, but special thanks to the Sieber lab at TUM for hosting me in Munich to learn and perform these chemoproteomic experiments! Lipoprotein trafficking is clearly an attractive target for gram(-)’s, especially for beta-lactamase expressing bacteria.
We then demonstrated that OXA-23, the beta-lactamase that confers resistance, is itself an outer-membrane lipoprotein. Inhibiting lipoprotein trafficking via LolF thus also causes over-accumulation of OXA-23 to the inner membrane, which exaggerates these inhibitor’s antibiotic properties.
June 29, 2025 at 5:36 PM
We then demonstrated that OXA-23, the beta-lactamase that confers resistance, is itself an outer-membrane lipoprotein. Inhibiting lipoprotein trafficking via LolF thus also causes over-accumulation of OXA-23 to the inner membrane, which exaggerates these inhibitor’s antibiotic properties.
To understand this unique effect, we used a photoaffinity probe of this HTS hit to assign the Lol complex as the protein target of this compound! Resistance selection, Lol overexpression, and CRISPR studies also confirm lipoprotein trafficking as the major MOA.
June 29, 2025 at 5:36 PM
To understand this unique effect, we used a photoaffinity probe of this HTS hit to assign the Lol complex as the protein target of this compound! Resistance selection, Lol overexpression, and CRISPR studies also confirm lipoprotein trafficking as the major MOA.
literally just had to do this for all my procedures for an SI… add this to the list of things AI could probably do that would be useful 🥲
March 22, 2025 at 8:00 PM
literally just had to do this for all my procedures for an SI… add this to the list of things AI could probably do that would be useful 🥲
never used it, but always thought the Beckmann rearrangement was a neat and weird one
March 14, 2025 at 1:24 AM
never used it, but always thought the Beckmann rearrangement was a neat and weird one
Thanks Dr. Rouhier! Hope all is well back on the hill :)
February 25, 2025 at 6:07 PM
Thanks Dr. Rouhier! Hope all is well back on the hill :)
tbh I’m trying to be both at the same time
February 22, 2025 at 6:54 PM
tbh I’m trying to be both at the same time
yikes same, didn’t realize this…
February 11, 2025 at 3:11 AM
yikes same, didn’t realize this…
pubs.acs.org/doi/abs/10.1...
Also Amaechi Odoh (currently at Abbvie I think? Was at Clemson) who published this killer SUFEX paper!!
Also Amaechi Odoh (currently at Abbvie I think? Was at Clemson) who published this killer SUFEX paper!!
SuFEx-Enabled Direct Deoxy-Diversification of Alcohols
We introduce a new use of sulfonyl fluoride as a bifunctional reagent that facilitates the one-step deoxy-diversification of complex alcohol libraries. Our reaction design features a Sulfur(VI) Fluoride Exchange (SuFEx) mediated activation of alcohols and fluoride-induced activation of silicon-bound nucleophiles. This method enables the direct conversion of alcoholic C–O bonds in complex molecules into diverse analogues via C–C, C–N, C–Cl, and C–Br bond formation while suppressing any elimination side-products.
pubs.acs.org
February 3, 2025 at 1:57 AM
pubs.acs.org/doi/abs/10.1...
Also Amaechi Odoh (currently at Abbvie I think? Was at Clemson) who published this killer SUFEX paper!!
Also Amaechi Odoh (currently at Abbvie I think? Was at Clemson) who published this killer SUFEX paper!!
@lmkdassama.bsky.social, who just published an awesome review on gram(-) antibiotic drug discovery!
February 3, 2025 at 1:49 AM
@lmkdassama.bsky.social, who just published an awesome review on gram(-) antibiotic drug discovery!
Corey ylide ftw, love that reagent
January 29, 2025 at 10:59 PM
Corey ylide ftw, love that reagent
Now this is civil disobedience 🔥🔥🔥
January 24, 2025 at 3:45 PM
Now this is civil disobedience 🔥🔥🔥
Bag of gloves and towels - figured it was something Sn!
January 21, 2025 at 8:57 PM
Bag of gloves and towels - figured it was something Sn!