Shosei Imai
@sikmys.bsky.social
Department of Chemistry
The University of Tokyo
Campbell Lab
PhD candidate
The University of Tokyo
Campbell Lab
PhD candidate
Reposted by Shosei Imai
High-performance genetically-encoded green and red fluorescent biosensors for pyruvate https://www.biorxiv.org/content/10.1101/2025.04.17.649293v1
April 18, 2025 at 3:17 AM
High-performance genetically-encoded green and red fluorescent biosensors for pyruvate https://www.biorxiv.org/content/10.1101/2025.04.17.649293v1
Reposted by Shosei Imai
New tools! GreenPy and ApplePy are fluorescent biosensors for pyruvate with huge responses (20–40x in vitro) and a range of affinities (10s of μM to mM). These should be game changers for imaging of metabolism! Great work by Shosei Imai @sikmys.bsky.social and team 👏. www.biorxiv.org/content/10.1...
High-performance genetically-encoded green and red fluorescent biosensors for pyruvate
Pyruvate is the end-product of glycolysis and a central metabolite involved in many biochemical pathways. However, a lack of high-performance (i.e., Δ F / F > 10) single fluorescent protein (FP)-base...
www.biorxiv.org
April 19, 2025 at 6:42 AM
New tools! GreenPy and ApplePy are fluorescent biosensors for pyruvate with huge responses (20–40x in vitro) and a range of affinities (10s of μM to mM). These should be game changers for imaging of metabolism! Great work by Shosei Imai @sikmys.bsky.social and team 👏. www.biorxiv.org/content/10.1...